Wyniki wyszukiwania - Biblioteka Nauki

1

Expression of a constitutively active mutant of heat shock factor 1 under the control of testis-specific hst70 gene promoter in transgenic mice induces degeneration of seminiferous epithelium.

100%

Widłak W. , Benedyk K. , Vydra N. , Głowala M. , Ścieglińska D. , Małusecka E. , Nakai A. , Krawczyk Z.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 2

535-541

EN

Heat shock activates in somatic cells a set of genes encoding heat shock proteins which function as molecular chaperones. The basic mechanism by which these genes are activated is the interaction of the specific transcription factor HSF1 with a regulatory DNA sequence called heat shock element (HSE). In higher eukaryotes HSF1 is present in unstressed cells as inactive monomers which, in response to cellular stress, aggregate into transcriptionally competent homotrimers. In the present paper we showed that the expression of a transgene encoding mutated constitutively active HSF1 placed under the control of a spermatocyte-specific promoter derived from the hst70 gene severely affects spermatogenesis. We found the testes of transgenic mice to be significantly smaller than those of wild-type males and histological analysis showed massive degeneration of the seminiferous epithelium. The lumen of tubules was devoid of spermatids and spermatozoa and using the TUNEL method we demonstrated a high rate of spermatocyte apoptosis. The molecular mechanism by which constitutively active HSF1 arrests spermatogenesis is not known so far. One can assume that HSF1 can either induce or repress so far unknown target genes involved in germ cell apoptosis.

2

The GC-box is critical for high level expression of the testis-specific Hsp70.2/Hst70 gene

100%

Widłak W. , Vydra N. , Dudaladava V. , Ścieglińska D. , Winiarski B. , Krawczyk Z.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 1

107-112

EN

The Hsp70.2/Hst70 gene, which belongs to the 70 kDa heat-shock protein (HSP) family, is expressed specifically in primary spermatocytes and spermatids. The regulatory elements required for a high level of testis-specific expression of the gene are placed between the two major transcription start sites T1 and T2 (approximately 350 and 115 bp upstream of the starting ATG codon). Here we have shown that sequences proximal to the exon1/intron splicing site in the 5' untranslated region of the Hsp70.2/Hst70 gene, which include a highly conserved element called box B, are required for efficient expression of the chloramphenicol acetyltransferase reporter gene in testes of transgenic mice. However, in spite of the drastically reduced overall activity, the stage-specific expression pattern of the transgene was preserved after removal of these sequences. We have also shown that GC-box located downstream of the box B (approximately 210 bp upstream of the starting ATG codon) is indispensable for efficient expression of the Hsp70.2/Hst70 gene promoter in spermatogenic cells. The GC-box specifically binds proteins present in nuclear extracts from testes (putatively Sp1-like factors). A change in the pattern of such GC-box-interacting factors corresponds to activation of the Hsp70.2/Hst70 gene, confirming the importance of this regulatory element.

3

Annual reproductive cycle in two free living populations of three-spined stickleback (Gasterosteus aculeatus L.): patterns of ovarian and testicular development

100%

Sokołowska E. , Kulczykowska E.

Oceanologia

|

2006

|

tom No. 48 (1)

103-121

EN

The annual reproductive cycle in two wild populations of three-spined stickleback was studied. Sticklebacks from the Dead Vistula River (Martwa Wisła) (brackish water) and the Oliva Stream (Potok Oliwski) (freshwater) were exposed to annual environmental changes in their natural habitats. Ovaries and livers (females), and testes and kidneys (males) were collected during 1-2 years. The gonadosomatic IG, hepatosomatic IH, nephrosomatic IN indices, kidney epithelium height (KEH) and size of oocytes were calculated.The number of mature oocytes and percentage of ovulating females were determined during the spawning season. Histological changes in the ovaries and testes were described throughout a year. Annual reproductive cycles were similar in both populations of sticklebacks. This is the first histological and morphological study carried out throughout a year, simultaneously in two wild populations of three-spined sticklebacks inhabiting different environments. An improved scale of gonadal development in conjunction with the determined indices and fecundity give a comprehensive description of the reproductive cycle. These new observations, in combination with previously reported features, provide a universal scale that can be successfully used to distinguish all phases of gametogenesis in sticklebacks in different habitats.

4

Tracking chromatin states using controlled DNase I treatment and real-time PCR

100%

Martins R. P. , Platts A. E. , Krawetz S. A.

|

tom 12

|

nr 4

545-555

EN

A novel approach to DNase I-sensitivity analysis was applied to examining genes of the spermatogenic pathway, reflective of the substantial morphological and genomic changes that occur during this program of differentiation. A new real-time PCR-based strategy that considers the nuances of response to nuclease treatment was used to assess the nuclease susceptibility through differentiation. Data analysis was automated with the K-Lab PCR algorithm, facilitating the rapid analysis of multiple samples while eliminating the subjectivity usually associated with Ct analyses. The utility of this assay and analytical paradigm as applied to nuclease-sensitivity mapping is presented.

5

Effect of caffeine on spermatogenesis in Chara vulgaris L.

100%

Jaros K. , Maszewski J.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

|

1997

|

tom 39

|

nr 1

6

Autoradiographic studies on mucilage synthesis in Chara vulgaris antheridium with the use of 3H-fucose in total darkness and light

100%

Gosek A.

Folia Histochemica et Cytobiologica

|

1996

|

tom 34

|

nr 3-4

7

Actin cytoskeleton organization and dynamics in Drosophila melanogaster spermatid individualization

86%

Lenartowska M. , Noguchi T. , Frank D. J. , Miller K. G.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2008

|

tom 50

|

nr 1

8

The role of calcium in signal transduction processes in Sertoli cells

86%

Gorczynska-Fjalling E.

Reproductive Biology

|

2004

|

tom 04

|

nr 3

9

Genistein alleviates testicular ischemia and reperfusion injury-induced spermatogenic damage and oxidative stress by suppressing abnormal testicular matrix metalloproteinase system via the Notch 2/Jagged 1/Hes-1 and caspase-8 pathways

86%

Al-Maghrebi M. , Renno W. M.

Journal of Physiology and Pharmacology

|

2016

|

tom 67

|

nr 1

10

Cadmium effect on the cytophores structure in seminal vesicles in the earthworm Dendrobaena veneta (Rosa)

86%

Siekierska E. , Brzozowa M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2008

|

tom 50

|

nr 1

11

Course of spermatogenesis in Herpobdella octooculata L. in natural environment and after administration of cadmium

86%

Holdakowska-Deja T.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

|

1997

|

tom 39

|

nr 1

12

Detection of DAZmRNA distribution in human testis using reserve transcription in situ PCR technique [RT-ISPCR]

86%

Warchol J. B. , Augustyniak S. , Stecewicz D. , Jankowska A.

Folia Histochemica et Cytobiologica

|

2001

|

tom 39

|

nr 2

13

Changes in ultrastructure of cytoplasm and nucleus during spermiogenesis in Chara vulgaris

86%

Kwiatkowska M.

Folia Histochemica et Cytobiologica

|

1996

|

tom 34

|

nr 1

14

Precocious maturation of males of the sea trout [Salmo trutta L.] age 0 grown in natural conditions

86%

Dziewulska K.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

|

1997

|

tom 39

|

nr 1

15

Thickness and structure changes of lobule boundary wall of testis of precocious mature male of sea trout [Salmo trutta m.trutta L.] during the end of first spermatogenesis

86%

Dziewulska K.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2000

|

tom 42

|

nr 1

16

Ultrastructure of spermatogenesis of the anoplocephalid cestode Gallegoides arfaai [Mobedi et Ghadirian, 1977] Tenora et Mas-Coma, 1978

86%

Miquel J. , Swiderski Z. , Mlocicki D. , Eira C. , Marchand B.

Acta Parasitologica

|

2005

|

tom 50

|

nr 2

EN

Spermatogenesis in Gallegoides arfaai is similar to that described for other cestode species. Six incomplete synchronic cytokineses occur: four mitotic and two meiotic cell divisions. The primary spermatogonium divides forming two secondary spermatogonia. All further divisions occur simultaneously, resulting in a rosette of four tertiary, then eight quaternary spermatogonia and sixteen primary spermatocytes. The first meiotic division forms thirty-two secondary spermatocytes and after the second meiotic division sixty-four spermatids are formed. Spermiogenesis begins with the formation of a differentiation zone in the form of a conical projection of cytoplasm delimited by a ring of arching membranes. Within this area there are two centrioles, a centriolar adjunct and vestigial striated rootlets. During spermiogenesis, only one of the centrioles develops an axoneme that grows directly into the cytoplasmic extension. The other centriole remains oriented in a cytoplasmic bud and posteriorly aborts. The nucleus elongates and moves into the cytoplasmic extension. Granular material present in each sperm originates from electron-dense material present in the periphery of the spermatid. In the final stage of spermiogenesis two crest-like bodies appear at the Bâse of the spermatid. Finally, the ring of arching membranes constricts and the young spermatozoon detaches from the residual cytoplasm. In order to increase homogeneity in the designation of the non-typical striated rootlets previously described, in this study we propose to group them under the common designation of "vestigial striated rootlets" and its importance is discussed according to previous findings of related structures in other cyclophyllideans.

17

Postnatal development of the European bison spermatogenesis

86%

Czykier E. , Sawicki B. , Krasinska M.

|

tom 44

|

nr 1

EN

The testes and epididymes of the European bison Bison bonasus (Linnaeus, 1758) were collected from culled animals living in free-ranging populations in Białowieża Forest, Borecka Forest and Bieszczady Mountains, and in captivity (103 males, 4 months to 17 years old). We found that all bulls were sexually mature at the age of 4 years, and some even at the age of 3 years. Animals which started mature spermatozoa production were treated as sexually mature. We observed the inhibition of spermatogenesis efficiency and involutionary changes in epididymal epithelium after 12 years of age. Therefore, in the postnatal development of spermatogenesis three phases were distinguished: I - development of spermatogenesis (4th month - 4th year of age), II - mature spermatogenesis (4th - 12th year), III - inhibition of spermatogenesis efficiency (over 12th year of age).

18

Flow cytometric analysis of spermatogenesis in triploid Rana esculenta

86%

Brychta B. H. , Tunner H. G.

Zoologica Poloniae

|

1994

|

tom 39

|

nr 3-4

19

Interactions of rat repetitive sequence MspI8 with nuclear matrix proteins during spermatogenesis

86%

Rogolinski J. , Widlak P. , Rzeszowska-Wolny J.

Acta Biochimica Polonica

|

1996

|

tom 43

|

nr 2

EN

Using the Southwestern blot analysis we have studied the interactions between rat repetitive sequence MspI8 and the nuclear matrix proteins of rat testis cells. Starting from 2 weeks the young to adult animals showed differences in type of testis nuclear matrix proteins recognizing the MspI8 sequence. The same sets of nuclear matrix proteins were detected in some fractions enriched in spermatocytes and spermatides and obtained after fractionation of testis cells of aduit animals by the velocity sedimentation technique.

20

Mitochondria activity and distribution during spermatogenesis in the earthworm Dendrobaena veneta male germ-cell clusters

72%

Malota K. , Swiatek P.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2016

|

tom 58

|

nr 1