Wyniki wyszukiwania - Biblioteka Nauki

1

Is concentration and motility of male gametes related to DNA damage measured by comet assay?

100%

Dobrzynska M. M. , Tyrkiel E. J. , Derezinska E. , Ludwicki J. K.

Annals of Agricultural and Environmental Medicine

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2010

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tom 17

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nr 1

73-77

2

Air pollution and semen parameters in men seeking fertility treatment for the first time

100%

Wdowiak A. , Wdowiak E. , Bień A. , Bojar I. , Iwanowicz-Palus G. , Raczkiewicz D.

International Journal of Occupational Medicine and Environmental Health

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2019

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tom 32

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nr 3

387-399

EN

Objectives The purpose of this paper was to analyze the relationship between the selected chemical air pollutants found in the Lublin Province and the semen parameters of men seeking fertility treatment for the first time. Material and Methods The study involved an analysis of semen sample test results obtained from male patients first reporting for fertility treatment in reproductive health centers in the Lublin Province, Poland. The data set comprises semen parameters of 13 148 men, and the number of samples in the reference period was 255–769 annually. Data on air pollution were obtained from the website of the Polish General Environmental Inspectorate and included selected chemical air pollutant levels, i.e., NO₂, SO₂, O₃, and PM₁₀. Results The mean PM₁₀ levels in the air increased, on average, by 0.65 μg/m₃ annually in 2000–2015 (p = 0.029); the mean levels of O₃, NO₂, SO₂ did not change significantly in the analyzed period. There were increasing trends in the mean sperm density and total sperm count of the subjects in 1992–2015 (p < 0.001). The mean percentage of sperm with normal morphology significantly decreased in the subsequent analysis periods: 1992–1998 (p = 0.001); 1999–2009 (p < 0.001); 2010–2015 (p = 0.001). A significant negative correlation was found between the ozone levels in the air in the Lublin Province and the percentage of sperm with normal morphology (r = –0.8311, p = 0.040). Conclusions Exposure to ozone in the air contributes to decreased percentages of sperm with normal sperm morphology. Over the years, there was an increasing trend in sperm density in the men first reporting for fertility treatment, and a decreasing trend in the percentage of sperm with normal morphology. Int J Occup Med Environ Health. 2019;32(3):387–99

3

Quality parameters and fertility of ram semen cryopreserved in egg yolk and soybean lecithin supplemented extenders

100%

Gogol P. , Bryła M. , Trzcińska M. , Bochenek M. , Gogol P. , Bryła M. , Trzcińska M. , Bochenek M.

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4

The influence of selected factors on the beef bulls sperm quality used for artificial insemination

100%

Kunowska-Slosarz M. , Przysucha T. , Golebiewski M. , Slosarz J. , Balcerek M.

Electronic Journal of Polish Agricultural Universities. Series Animal Husbandry

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2015

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tom 18

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nr 1

5

Evaluation of two prototype directional freezing methods and a 2ml flattened straw for cryopreservation of boar semen

100%

Puglisi R. , Bornaghi V. , Severgnini A. , Vanni R. , Montedoro M. , Galli A.

Animal Science Papers and Reports

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2017

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tom 35

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nr 4

EN

Based on previous assessments on stallions, 40 ejaculates of 20 Duroc boars were split and evenly frozen with a conventional vapour freezing method and two directional, drum and directional, prototype methods using commercial extenders and relative standard procedures. The directional prototype was provided with a double internal setup that allowed the positioning of experimental 2ml flat straws with 1 billion sperm (Flat) in a fixed support, or both classical 0.5 ml paillettes with 250 million spermatozoa and flats in a rotating drum designed so as to ensure a more uniform heat exchange. Preliminary tests for individuation of the most appropriate thawing rate showed beneficial effects (P≤0.05) of thawing the sperm at 50°C for 13 s when compared to 42°C for 20 s, in terms of total motility (42.8±8.4% and 35.6±6.8%, respectively). With regard to freezing/packaging methods, major improvements (P≤0.05) were shown for the drum method with paillettes for total motility (38.6±14.2%) assessed immediately after thawing, when compared with the conventional (29.4±13.3%) and the directional methods with flats (30.2±12.8%), and for total motility (P≤0.01) assessed following incubation for 120 min at 37°C after thawing (24.8±11.6%) with respect to the conventional method (15.6±10.9%). Despite the statistical non-significance of results, both the prototype freezing approaches using the experimental flat straw showed some improvements in functional parameters assessed by cytofluorometry when compared to the conventional method.

6

High levels of bulky DNA adducts in human sperm correlate with impaired fertility

100%

Horak S. , Polanska J. , Widlak P.

Acta Biochimica Polonica

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2003

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tom 50

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nr 1

EN

Progressive decline in fertility and sperm quality has been reported over the last few decades, especially in industrialized nations. It has been proposed that exposure to factors that induce damage in DNA of spermatogenic cells may significantly contribute to impaired fertility. Here, the 32P-postlabelling method was used to analyze the levels of bulky DNA adducts in sperm cells in a group of 179 volunteers, either healthy subjects or patients with an impaired fertility. The levels of DNA adducts were 1.35-fold higher in the infertile group as compared to healthy individuals (P = 0.012). Similarly, a significant negative correlation between the levels of DNA adducts and measures of semen quality (sperm concentration and motility) has been observed (P < 0.001). In addition, the levels of bulky DNA adducts in sperm cells positively correlates with amounts of leukocytes in semen, which were significantly higher in semen of infertile subjects.

7

Effect of low temperature thawing procedure and post-thaw cold shock on frozen bull semen

84%

Nur Z. , Segirkaya H. , Dogan I. , Soylu M. K. , Ak K. , Ileri I. K.

Medycyna Weterynaryjna

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2005

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tom 61

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nr 09

8

Effects of litter standardization on boar sperm quality and testes size

84%

Owsianny J. , Czarnecki R. , Wejksza D.

Advances in Agricultural Sciences

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1993

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tom 02

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nr 1

9

Female infertility - effect of perinatal xenoestrogen exposure on reproductive functions in animals and humans

84%

Gotz F. , Dorner G.

Folia Histochemica et Cytobiologica. Supplement

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2001

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tom 39

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nr 1

10

Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen

67%

Fraczek M. , Kurpisz M.

Folia Histochemica et Cytobiologica

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2015

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tom 53

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nr 3

11

KIR, LILRB and their ligands’ genes as potential biomarkers in recurrent implantation failure

67%

Nowak I. , Wilczynska K. , Wilczynski J. R. , Malinowski A. , Radwan P. , Radwan M. , Kusnierczyk P.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

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nr 5

12

The effects of di-n-butyl phthalate on the germ cells of laboratory mice

67%

Dobrzynska M. M. , Tyrkiel E. J. , Hernik A. , Derezinska E. , Goralczyk K. , Ludwicki J. K.

Roczniki Państwowego Zakładu Higieny

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2009

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tom 60

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nr 4

EN

Phthalate are found in the environmental samples due to their wide use in the industry as plasticizers. Di-n-butyl phthalate (DBP) is mainly used in nitrocellulose and polyvinyl acetate products as well as in personal-care products. This study was performed to investigate the influence of exposure to DBP on the quantity and quality (motility, morphology) and DNA damage (induction of micronuclei and DNA strand breaks) of male mice gametes. The estimation of DBP residues was also done. Eight weeks exposure to DBP (500 mg/kg bw and 2000 mg/kg bw) did not significantly affect testes and epididymes weights as well as sperm count. DBP clearly diminished sperm motility, enhanced frequency of abnormal sperm heads and not significantly increased DNA strand breaks in germ cells as well as frequency of micronuclei in spermatids. There were no bioacumulation of DBP in mice. Results suggest that DBP may affect the male mice germ cells.

PL

Ftalany są powszechnie wykorzystywane w przemyśle jako plastyfikatory, dlatego też często występują w próbach środowiskowych. Ftalan di-n-butylu (DBP) jest składnikiem produktów nitrocelulozowych i poliwinylowych, jak również produktów do higieny osobistej. Celem pracy było zbadanie wpływu podawania DBP na ilość i jakość gamet męskich myszy (ruchliwość, morfologia) oraz na występowanie uszkodzeń DNA (indukcja mikrojąder oraz pęknięć nici DNA). Zbadano także stężenie pozostałości DBP w gametach. Ośmiotygodniowe narażanie na DBP (500 mg/kg mc i 2000 mg/kg mc) nie powodowało istotnych zmian w ciężarze jąder i najądrzy ani w liczebności plemników. Narażenie na DBP, zwłaszcza w dawce 2000 mg/kg mc wpływało wyraźnie na zmniejszenie ruchliwości plemników i zwiększenie odsetka plemników o nieprawidłowej budowie morfologicznej. Powodowało też nieznaczne zwiększenie pęknięć nici DNA w gametach oraz zwiększenie częstości występowania mikrojąder w spermatydach. Nie wykazano bioakumulacji DBP w gonadach samców myszy. Uzyskane wyniki sugerują, że DBP może oddziaływać niekorzystnie na męskie komórki rozrodcze myszy.

13

New insights about the evaluation of human sperm quality: the aromatase example

67%

Galeraud-Denis I. , Travert C. , De Vienne C. , Said L. , Saad A. , Carreau S.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 5

14

FSHbeta gene mutation in a female with delayed puberty and hypogonadism: response to recombinant human FSH

67%

Kottler M. L. , Richard N. , Chabre O. , Alain S. , Young J.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 5

15

Influence of heavy metals and 4-nonylphenol on reproductive function in fish

59%

Popek W. , Dietrich G. , Glogowski J. , Demska-Zakes K. , Drag-Kozak E. , Sionkowski J. , Luszczek-Trojan E. , Epler P. , Demianowicz W. , Sarosiek B. , Kowalski R. , Jankun M. , Zakes Z. , Krol J. , Czerniak S. , Szczepkowski M.

Reproductive Biology

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2006

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tom 06

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nr Suppl.1