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1

DEVELEOPMENT AND VALIDATION OFSPECTROFLUOROMETRIC AND SPECTROPHOTOMETRIC BIVARIATE METHODS FOR SIMULTANEOUS DETERMINATION OF PROPRANOLOL AND HYDTOCHLOROTHIAZIDE IN DRUG TABLETS

100%

Wedian F. , AbuAlrob R.

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nr 3

453-463

EN

Spectrofluorometric and spectrophotometric bivariate methods were developed for quantitative of Propranolol and Hydrochlorothiazide in drug tablets in aqueous medium. The methods were tested according to ICH guidelines for linearity, recovery, and specificity and were found accurate, precise, sensitive, and free from interferences. The linearity was established over the ranges 0.025–0.30 and 0.010–0.20 mg/L in the first method, and 1.0–20.0 and 5.0–25.0 mg/L in second method, for PRO and HCT, respectively. The excitation and emission wavelengths were 260, 345 and 270, 415 nm, for PRO and HCT in the first method, respectively. The recoveries were higher than 95% for both methods. Additionally, the results were statistically compared to that of standard chromatographic methods and showed that no significant difference among the methods regarding both accuracy and precision. Thus, the methods were successfully applied to quantitation of PRO and HCT in combined formulations. The proposed methods are reliable, simple, sensitive, and adaptable and could effectively be an alternative method to HPLC.

2

Oznaczenie histaminy w konserwach rybnych metodą fluorymetryczną, przy użyciu aparatu Spekol-10 z przystawką FK do pomiaru fluorescencji

86%

Fonberg-Broczek M. , Sawilska-Rautenstrauch D. , Windyga B.

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nr 1

3

Coelomocytes and fluorophores of the earthworm Dendrobaena veneta raised at different ambient temperatures

72%

Cygal M. , Lis U. , Kruk J. , Plytycz B.

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tom 49

5-11

4

Produkty zbozowe jako zrodlo selenu w krajowych racjach pokarmowych

58%

Marzec Z.

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tom 53

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nr 4

377-383

PL

Przeprowadzono badania zawartości selenu w rynkowych produktach zbożowych oraz całodobowych racjach pokarmowych. W analizie zastosowano metodę spektrofluorymetryczną z wykorzystaniem 2,3-diaminonaftalenu. Oceniono ilościowy udział selenu pochodzącego z różnych grup produktów spożywczych w całodobowej puli tego pierwiastka.

EN

The aim of the study was to update the data concerning the level of selenium in cereal products and to determine its uptake with daily food rations and percentage of cereal products in the 24-hour pool of selenium ingested. Selenium was determined fluorometrically after the reaction of Se (IV) with 2,3-diaminonaphthalene and extraction of naphtho-[2,3-d]-2-seleno-1,3-diazole to cyclohexane. Compared to the results found several years ago, the selenium levels observed in cereals, flour and bakery products were significantly lower. The mean levels of selenium in the above - mentioned products ranged from 22,9 µg/kg to 53,0 µg/kg and were markedly lower compared with pasta which reach to level 528 µg/kg, especially when pasta was made from semoline or contained eggs. The average daily intake of selenium with reconstructed rations was 37,9 µg while with home made rations 60,4 µg. The values calculated on the basis of the data collected in the 80-ties were significantly stray to analytical results, and those based on our findings was comparable. The percentage of the most important products in the daily selenium pool changed significantly after taking into account these new results because according to the data from 80-ties, the major sources of selenium in home and reconstructed rations were cereal products which share to 48%. According to the new results the main source of selenium in daily rations were meat one its products about 26% and cereal products somewhat less than 20%.

5

Changes in coelomocytes of the earthworm, Dendrobaena veneta, exposed to cadmium, copper, lead or nickel-contaminated soil [Short communication]

58%

Kwadrans A. , Litwa J. , Woloszczakiewicz S. , Ksiezarczyk E. , Klimek M. , Duchnowski M. , Kruk J. , Plytycz B.

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tom 50

57-62

6

Spektralne metody oznaczania sladowych ilosci selenu w wodach naturalnych oraz sokach owocowych

58%

Gawloska A. , Maslowska J.

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nr 1

91-97

PL

W krajowych naturalnych wodach mineralnych oraz sokach owocowych oznaczano śladowe zawartości selenu wykorzystując trzy niezależne metody spektralne: 1 -metodę absorpcyjnych widm atomowych (ASA); 2-metodę spektrofotometrii absorpcyjnej (UV - VIS) oraz 3-metodę spektrofluorymetryczną (SF). Badania nasze wykazały, że zawartość selenu w badanych próbkach jest bardzo mała i nie przekracza poziomu 0,464 µg/dm3.

EN

Considering the essential nutritional role of selenium and the necessity to assess its contribution to the environmental risk, the problem of reliable determination of selenium in typical food products continues to be very important. In this work, the authors determined trace quantities of selenium in various commercial natural mineral waters and fruit juices by three independent methods: 1) atomic absorption spectrometry (AAS), 2) spectrophotometry (UV-VIS), and 3) spectrofluorometry (SF). A Prolabo (France) MX 350 Maxidigest microwave oven was used to mineralise the samples. Hydrochloric acid and 3,3'-diaminobenzidine (DAB), which forms violet/yellow-coloured piazoselenium with SeO32-, were added to the colourless clear mineralisates. Absorbancy readings of the coloured toluene solutions were taken at the wavelength of λ = 420 nm (UV-VIS) or 265/563 nm (SF). With the AAS method, SeO32- -containing mineralisates were directly analysed. Selenium content in the analysed samples was very low, within 0.464 µg/l. The results also show a considerable variation in selenium content of the examined natural mineral waters and fruit juices.