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EN
The objective of this study was to investigate the effect of low-level laser therapy (LLLT) on the proliferation of epithelial and connective tissue cells in the healing of incised cutaneous wounds in pigs. The experiment was conducted on 12 young pigs divided into four groups. Group I (undamaged skin) and group III (damaged skin) served as control. Group II - pigs with undamaged skin and group IV - pigs with incised wounds in the dorsal area were subjected to laser irradiation. Laser biostimulation was carried out using a CTL 1106 MX semiconductor laser in the continuous wave mode of operation at a wavelength of 810 nm and a maximum output of 100 mW. Following three weeks of observation, skin specimens were collected for histopathological analysis (HE), immunohistochemical detection of PCNA, and determination of apoptosis (TUNEL) and presence of mast cells (toluidine blue staining). Laser irradiation administered at E=8 J/cm for 1 min over a period of three weeks accelerated the proliferation of stratum basale cells, stimulated fibroblast proliferation, increased the number of mast cells in the wound area, and inhibited apoptosis in cells participating in the skin regeneration process.
EN
The objective of this study was to investigate the impact of therapeutic laser irradiation on wound healing and skin regeneration in pigs as a supplementary treatment. The experiment was conducted on 32 young pigs divided into four groups: groups I and III served as control, group II comprised pigs with undamaged skin, and group IV consisted of pigs with cutaneous surgical wounds in the dorsal area. Groups II and IV were subjected to laser irradiation. Laser biostimulation was carried out using a CTL 1106 MX semiconductor laser in the continuous wave mode of operation at a wavelength of 810 nm and a maximum output of 100 mW. Following three weeks of observation and clinical skin trials, specimens for a histopathological analysis were collected. The reported results indicate that laser treatment shortens the wound healing process by speeding up the growth of granulation tissue and improving skin elasticity. Laser irradiation of the skin in pigs increases cellular infiltration of the corium and stimulates the proliferation of the stratum germinativum cells of the epidermis. Laser irradiation may be recommended as supplementary therapy in the treatment of surgical wounds.
EN
Degradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.
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Content available remote Właściwości światła niebieskiego
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tom T. 21, cz. 2
37--46
PL
Światło niebieskie stanowi składową widma promieniowania słonecznego oraz widm wielu źródeł sztucznych. Oprócz generowania wrażeń wzrokowych, ma zdolność oddziaływania na organizm człowieka jeszcze w innym charakterze. Doniesienia naukowe stwierdzają możliwość wpływu światła niebieskiego m.in. na przebieg rytmu biologicznego, na samopoczucie osób starszych, na proces leczenia zmian skórnych. Jednak najlepiej rozpoznanym aspektem oddziaływania światła niebieskiego na człowieka jest jego zdolność do wywoływania uszkodzeń siatkówki oka. W środowisku pracy, domowym i komunalnym stosuje się liczne źródła technologiczne czy źródła oświetleniowe nowej generacji, które w swoim widmie zawierają światło niebieskie, dlatego światło to można uznać za powszechnie występujący czynnik, mogący zagrażać zdrowiu człowieka. W artykule przytoczono wyniki przeglądu literatury oraz informacji w mediach o oddziaływaniu światła niebieskiego na człowieka, celem przybliżenia tego tematu szerszej grupie społeczeństwa.
EN
Blue light is a component of the sun and many artificial sources’ radiation spectrum. This light has capacity for not only creation of visual impression but also affecting the human organism otherwise. In accordance with scientific reports, the blue light may have effect on biological rhythm, elderly persons condition, treatment of skin diseases. But its capacity for retina’s injury is recognized in the best way. There are many technological or modern lighting sources at work, domestic and municipal environment, which emit blue light. We can therefore say that blue light is a common existing factor and unexpectedly may pose a threat to human health. In this article, the review of literature and media information about blue light are reported, to acquaint the society with this subject.
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