Wyniki wyszukiwania - Biblioteka Nauki

1

Microarray of SNPs for diverse applications in Holstein-Friesian cattle breeding

100%

Kaminski S. , Brym P. , Ahman A. , Rusc A. , Wojcik E.

Annals of Animal Science

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2006

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tom 06

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nr 2

2

Molecular identification of blast resistance genes in rice genotypes using gene-specific markers

100%

Al-Daej M. I. , Ismail M. , Rezk A. A. , El-Malky M. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2019

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tom 100

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nr 3

3

Association between polymorphisms in CHRNA3 and PHACTR2 gene and environment and NSCLC risk in Chinese population

100%

Lou G. , Zhang Y. , Bao W. , Deng D.

Acta Biochimica Polonica

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2014

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tom 61

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nr 4

765-768

EN

Aims. This study aimed to investigate CHRNA3 (rs8040868) and PHACTR2 (rs9390123) single-nucleotide polymorphisms (SNPs) for association with non-small-cell lung cancer (NSCLC) risk in a Chinese population, and whether the environment affects the genetic polymorphisms. Methods. This case and control study included 500 NSCLC patients and 500 age-matched healthy controls. CHRNA3 (rs8040868) and PHACTR2 (rs9390123) SNPs were genotyped and associated for NSCLC risk by computing the odds ratio and 95% confidence interval from multivariate unconditional logistic regression analyses with adjustment of age. Results. The minor allele frequency (MAF) of CHRNA3 (rs8040868) and PHACTR2 (rs9390123) was 0.350 (C) and 0.397 (C), respectively. The frequencies of genotype and allele in CHRNA3 (rs8040868) and PHACTR2 (rs9390123) were not significantly different between the cases and controls, or between either of the subgroups. Conclusion. Although rs8040868 and rs9390123 SNPs are not associated with NSCLC risk in Chinese population, the results strongly suggest that geographical agents interact with human genetic polymorphism independent of ethnic background.

4

Database of SNPs in candidate genes potentially associated with yield and quality of pork

100%

Brym P. , Kaminski S.

Animal Science Papers and Reports

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2006

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tom 24

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nr 3

5

Evaluation of lectin pathway activity and mannan-binding lectin levels in the course of pregnancy complicated by diabetes type 1, based on the genetic background

100%

Pertynska-Marczewska M. , Cedzynski M. , Swierzko A. , Szala A. , Sobczak M. , Cypryk K. , Wilczynski J.

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tom 57

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nr 3

221-228

6

Genetic diversity in Vernonia amygdalina Delile accessions revealed by random amplified polymorphic DNAs (RAPDs)

100%

Aikpokpodion P. , Abebe J. , Igwe D.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2018

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tom 99

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nr 2

7

The expression of bitter taste receptor TAS2R38 in patients with chronic rhinosinusitis

84%

Zborowska-Piskadlo K. , Stachowiak M. , Rusetska N. , Sarnowska E. , Siedlecki J. , Dzaman K.

Archivum Immunologiae et Therapiae Experimentalis

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2020

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tom 68

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nr 5

8

Identification of single nucleotide polymorphism within bactencin-5 and bactencin-7 coding genes in association with milk production traits

84%

Hiller S. , Kowalewska I.

Acta Scientiarum Polonorum. Zootechnica

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2022

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tom 21

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nr 4

9

Association between polymorphisms in CHRNA3 and PHACTR2 gene and environment and NSCLC risk in Chinese population

84%

Lou G. , Zhang Y. , Bao W. , Deng D.

Acta Biochimica Polonica

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2014

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tom 61

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nr 4

10

Use of chromosome walking in discovery of single-nucleotide polymorphism in noncoding regions of a candidate actin gene in Pinus radiata

84%

Li W. , Li H. , Wu H. , Chen X.-Y.

Journal of Applied Genetics

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2010

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tom 51

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nr 3

EN

Untranslated regions (UTRs) of eukaryotic mRNAs plav crucial roles in post-transcriptional regulation of gene expression via the modulation of nucleocytoplasmic mRNA transport, translation efficiency, subcellular localization, and message stability. Single-nucleotide polymorphisms (SNPs) in UTRs of a candidate gene may also change the post-transcriptional regulation of a gene or function by nucleotide mutation. For species that have not been entirely sequenced genomically, new methods need to be devised to discover SNPs in noncoding regions of candidate genes. In this study, based on the expressed sequence tag (EST) of Pinus radiata (Monterey pine), we obtained all the sequences of UTRs of the actin gene by using a chromosome walking method. We also detected all the SNPs in and around the coding region of the actin gene. In this way, the full genomie sequence (2154 bp) of the actin gene was identified, including the 5'UTR, introns, the coding sequence, and the 3'UTR. PCR amplification and DNA fragment sequencing from 200 unrelated P. radiata trees revealed a total of 21 SNPs in the actin gene, of which 3 were located in the 5'UTR, 3 in the introns, 10 in the coding sequence, and 5 in the 3'UTR. We show that chromosome walking can be used for obtaining the sequence of UTRs, and then, based on this sequence, to discover SNPs in the noncoding regions of candidate genes from this species without an entire genomic sequence.

11

Novel SNP of the goat prolactin gene [PRL] associated with cashmere traits

84%

Lan X.-Y. , Pan C.-Y. , Chen H. , Lei C.-Z. , Li F.-Y. , Zhang H.-Y. , Ni Y.-S.

Journal of Applied Genetics

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2009

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tom 50

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nr 1

51-54

EN

Concentrations of the single-chain polypeptide hormone prolactin (PRL) are associated with wool or cashmere traits, and its seasonal changes may determine patterns of enzymatic activity and may affect cashmere fibre growth and moult. So, the PRL gene is a potential candidate gene for cashmere traits in marker-assisted selection (MAS). In this paper, we report a novel missense single-nucleotide polymorphism (SNP) within the goat PRL gene in 1367 individuals by PCR-SSCP (polymerase chain reaction with single-strand conformation polymorphism) analysis and DNA sequencing. The novel X76049:g.576C>A mutation is confirmed by Eco241 PCR-RFLP (restriction fragment length polymorphism) analysis and causes a missense codon (Pro176Thr). The frequencies of allele C varied from 0.79 to 0.93 in 9 analysed goat populations. C allele was correlated with higher fibre length (P = 0.014).

12

Predictive value of ERCC1 single-nucleotide polymorphism in patients receiving platinum-based chemotherapy for locally-advanced and advanced non-small cell lung cancer — a pilot study

67%

Krawczyk P. , Wojas-Krawczyk K. , Mlak R. , Kucharczyk T. , Biernacka B. , Malinowski J.

Folia Histochemica et Cytobiologica

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2012

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tom 50

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nr 1

13

Microarray of SNPs for diverse applications in commercial pig breeding

67%

Kaminski S. , Brym P. , Help H. , Rusc A. , Wojcik E.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 1

EN

Modern pig production needs new tools for fast, reliable, more effective breeding. In the present paper we present a chip containing 45 SNP (Single Nucleotide Polymorphisms) which enables the determining of 1 genetic disease (PSS – Porcine Stress Syndrome), 4 QTLs genes: PRKAG3, CAST, MC4R and ESR, which together with the remaining SNPs create a panel useful in marker-assisted selection and veterinary control. The SNPs were genotyped using the PCR-APEX (Arrayed Primer Extension) technique. Special attention is paid to evaluation of the 45 SNP chip as an alternative approach to parentage and identity control. Based on allele frequency estimations, for a sample of 88 individuals of commercial pig lines, the probabilities that a randomly chosen candidate parent would be excluded from paternity or maternity were estimated to be 99.9% when genotypes of both parents and a progeny were known, and 98% when the genotypes of only one parent and a piglet were available. The marker set presented here also reached a probability of identity in the order of 10-16, which allows for unequivocal discrimination of animals or their products among billions of individuals. Further improvements for upcoming chip versions were also considered.

14

Isolation and molecular characterization of the porcine SLC6A14 gene excludes it as a candidate gene for fat deposition and growth

67%

Yang G. L. , Ren J. , Zhang S. H. , Huang W. B. , Guo Y. M. , Duan Y. Y. , Liu M. Z. , Huang L. S.

Journal of Applied Genetics

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2010

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tom 51

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nr 3

EN

The gene encoding solute carrier family 6 member 14 (SLC6A14) has been considered as a candidate gene affecting human obesity. In this study, full-length cDNA (2237 bp) and DNA sequence (24 541 bp) of the porcine SLC6A14 gene were isolated. The porcine SLC6A14 cDNA contains a 5'-untranslated region of 57 bp, a 3' -untranslated region of 254 bp, and an open reading frame of 1926 bp, encoding a deduced protein of 642 amino acids with a molecular mass of 72. 475 kDa and an isoelectric point of 7.82. The genomic structure of the porcine SLC6A14 gene is similar to mammalian orthologs, particularly in terms of exon size and exon/intron boundaries. It comprises 14 exons and 13 introns. A semi-quantitative RT-PCR showed that the porcine SLC6A14 mRNA expression was tissue-specific. Four SLC6A14 single-nucleotide polymorphisms (SNPs) were identified, and 3 informative SNPs were chosen for genotvping in a White Duroc × Erhualian resource population with phenotvpe data of growth and fatness traits. The association analysis showed that the c. 1438 G>A nonsynonymous polymorphism was associated with birth weight and 21-day body weight (P < 0.05), while g.7944 A>T was associated with 46-day body weight. Linkage and radiation hybrid mapping assigned SLC6A14 to a region around SW1522 on SSCXp13, which did not fall in the confidence interval of the quantitative trait locus (QTL) for growth and fatness traits on SSCX in the resource population. These results indicate that SLC6A14 is not a positional candidate gene for the QTL affecting fatness and growth traits in pigs.

15

Association between single-nucleotide polymorphisms of selected genes involved in the response to DNA damage and risk of colon, head and neck, and breast cancers in a Polish population

67%

Jelonek K. , Gdowicz-Klosok A. , Pietrowska M. , Borkowska M. , Korfanty J. , Rzeszowska-Wolny J. , Widlak P.

Journal of Applied Genetics

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2010

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tom 51

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nr 3

EN

Single-nucleotide polymorphisms in genes involved in DNA-damage-induced responses are reported frequently to be a risk factor in various cancer types. Here we analysed polymorphisms in 5 genes involved in DNA repair (XPD Asp312Asn and Lys751Gln, XRCC1 Arg399Gln, APE1 Aspl48Glu, NBS1 Glu185Gln, and XPA G-4A) and in a gene involved in regulation of the cell-cycle (CCND1 A870G). We compared their frequencies in groups of colon, head and neck, and breast cancer patients, and 2 healthy control groups: (1) matched healthy Polish individuals and (2) a NCBI database control group. Highly significant differences in the distribution of genotypes of the APE1, XRCC1 and CCND1 genes were found between colon cancer patients and healthy individuals. The 148Asp.APE1 allele and the 399Gln XRCC1 allele apparently increased the risk of colon cancer (OR = 1.9-2.3 and OR = 1.5-2.1, respectively). Additionally, frequencies of XPD genotypes differed between healthy controls and patients with colon or head and neck cancer. Importantly, no differences in the distribution of these polymorphisms were found between healthy controls and breast cancer patients. The data clearly indicate that the risk of colon cancer is associated with single-nucleotide polymorphism in genes involved in base-excision repair and DNA-damage-induced responses.