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Nuclear phenomena during autogamy in the marine ciliate Euplotes crassus: a tangled cytogenetic process fostering evolutionary conservatism

100%

Dini F. , Raikov I. B. , Bracchi P.

nr 1

Properties of the visually driven neurons of cat's hippocampal regions CA 1 and CA 3

84%

Harutiunian-Kozak B. A. , Hekimian A. A. , Turlejski K. , Kazarian A. L.

Acta Neurobiologiae Experimentalis

1989

tom 49

nr 1

Identification of a CAPA-PVK [Ixori-PVK] from single cells of the gulf Gulf Coast tick, Amblyomma maculatum

67%

Neupert S. , Russell W. K. , Russell D. H. , Strey O. F. , Teel P. D. , Strey A. , Nachman R. J.

nr 1-2

67-73

MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequence of a CAPA-PVK in the Gulf Coast tick, Amblyomma maculatum. Single cell analysis allowed the identification of the amino acid sequence of Ixori-PVK (PALIPFPRV-NH2), a periviscerokinin which had previously been identified from two other ticks, Ixodes ricinus and Boophilus microplus. The identification indicates greater conservation of sequence for the CAPA-PVK/CAP2b family in ticks as compared with insects. Side-chain fragmentation experiments provided data to distinguish between Leu/Ile ambiguities. The tick CAPA peptide shows a high sequence homology with other members of the insect periviscerokinin/ CAP2b peptides, which are associated with the regulation of critical physiological processes such as diuresis. Thus, the identification of this neuropeptide will provide the experimental basis to better understand regulation of water balance in these arthropods, providing a potential opportunity to develop neuropeptide-based control strategies against these livestock pests.

Oxidative damage to DNA and antioxidant status in aging and age-related diseases

67%

Olinski R. , Siomek A. , Rozalski R. , Gackowski D. , Foksinski M. , Guz J. , Dziaman T. , Szpila A. , Tudek B.

tom 54

nr 1

Aging is a complex process involving morphologic and biochemical changes in single cells and in the whole organism. One of the most popular explanations of how aging occurs at the molecular level is the oxidative stress hypothesis. Oxidative stress leads in many cases to an age-dependent increase in the cellular level of oxidatively modified macromolecules including DNA, and it is this increase which has been linked to various pathological conditions, such as aging, carcinogenesis, neurodegenerative and cardiovascular diseases. It is, however, possible that a number of short-comings associated with gaps in our knowledge may be responsible for the failure to produce definite results when applied to understanding the role of DNA damage in aging and age-related diseases.