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1 Bio-Algorithms and Med-Systems

1 2015

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The use of Titan yellow dye as a metal ion binding marker for studies on the formation of specific complexes by supramolecular Congo red

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Chłopaś K. , Jagusiak A. , Konieczny L. , Piekarska B. , Roterman I. , Rybarska J. , Stopa B. , Zemanek G. , Bielańska E. , Piwowar P. , Sadlik K.

Bio-Algorithms and Med-Systems

2015

tom Vol. 11, no. 1

9--17

Congo red (CR) and other self-assembling compounds creating supramolecular structures of rod- or ribbon-like architecture form specific complexes with cellulose and also with many proteins, including antibodies bound to the antigen and amyloids in particular. The mechanism of complexation and structure of these complexes are still poorly recognized despite the importance of the problem for medicine. This work proposes the progress in electron microscopy studies of amyloid-dye complexes by labeling supramolecular ligand CR with silver ions as a marker. Silver ions are introduced to CR carried by the strongly binding silver dye Titan yellow, which in addition form comicellar structures with CR. Silver carried by self-assembled dye molecules forms in the resulting metal nanoparticles, making the specific amyloid ligand CR perceptible in EM studies.