Wyniki wyszukiwania - Biblioteka Nauki

1

PDZ domains - common players in the cell signalling

100%

Jelen F. , Oleksy A. , Smietana K. , Otlewski J.

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nr 4

EN

PDZ domains are ubiquitous protein interaction modules that play a key role in cellular signaling. Their binding specificity involves recognition of the carboxyl-termi- nus of various proteins, often belonging to receptor and ion channel families. PDZ domains also mediate more complicated molecular networks through PDZ-PDZ interactions, recognition of internal protein sequences or phosphatidylinositol moieties. The domains often form a tandem of multiple copies, but equally often such tandems or single PDZ domain occur in combination with other signaling domains (for example SH3, DH/PH, GUK, LIM, CaMK). Common occurrence of PDZ domains in Metazoans strongly suggests that their evolutionary appearance results from the complication of signaling mechanisms in multicellular organisms. Here, we focus on their structure, specificity and role in signaling pathways.

2

Elements of signalling pathways in the response of Solanum species to Phytophthora infestans

75%

Polkowska-Kowalczyk L. , Olszak K. , Tarwacka J. , Szczegielniak J. , Muszynska G. , Wielgat B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

3

Contribution of protein kinase A and protein kinase C signalling pathways to the regulation of HSD11B2 expression and proliferation of MCF-7 cells

75%

Rubis B. , Grodecka-Gazdecka S. , Lecybyl R. , Ociepa M. , Krozowski Z. , Trzeciak W. H.

Acta Biochimica Polonica

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2004

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tom 51

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nr 4

EN

Contribution of the protein kinase A (PKA) and protein kinase C (PKC) signalling pathways to the regulation of 11y3-hydroxysteroid dehydrogenase type II (HSD11B2) gene expression was investigated in human breast cancer cell line MCF-7. Treatment of the cells with an adenylyl cyclase activator, forskolin, known to stimulate the PKA pathway, resulted in an increase in HSD11B2 mRNA content. Semi-quantitative RT-PCR revealed attenuation of the effect of forskolin by phorbol ester, tetra- decanoyl phorbol acetate (TPA), an activator of the PKC pathway. It was also demonstrated that specific inhibitors significantly reduced the effect of activators of the two pathways. Stimulation of the PKA pathway did not affect, whereas stimulation of the PKC pathway significantly reduced MCF-7 cell proliferation in a time-dependent manner. A cell growth inhibitor, dexamethasone, at high concentrations, caused a 40% decrease in proliferation of MCF-7 cells and this effect was abolished under conditions of increased HSD11B2 expression. It was concluded that in MCF-7 cells, stimulation of the PKA signal transduction pathway results in the induction of HSD11B2 expression and that this effect is markedly reduced by activation of the PKC pathway. Activation of the PKC pathway also resulted in inhibition of cell proliferation, while activation of the PKA pathway abolished the antiproliferative effect of dexamethasone. These effects might be due to oxidation of dexamethasone by the PKA-inducible HSD11B2.

4

IP6 modulates interleukin-8 secretion in colon epithelial cells by influencing various signalling pathways

75%

Weglarz L. , Wawszczyk J. , Orchel A. , Dzierzewicz Z.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.2

5

Contribution of the calcineurin signaling pathway to overload-induced skeletal muscle fiber-type transition

75%

Miyazaki M. , Hitomi Y. , Kizaki T. , Ohno H. , Haga S. , Takemasa T.

Journal of Physiology and Pharmacology

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2004

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tom 55

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nr 4

EN

Skeletal muscle is highly adaptable, being capable of undergoing changes in its structural and functional properties in response to physiological stimuli. The fast-to-slow muscle fiber-type transition is evoked by increased motor nerve activity. Recently, the calcineurin (CaN) signaling pathway has been implicated in the transcriptional regulation of slow muscle fiber genes. Here we investigated the effect of treatment with a CaN-specific inhibitor, FK506, on skeletal muscle fiber-type transition in functionally overloaded muscles. The overloaded plantaris muscle showed fast-to-slow muscle fiber type transition, i.e., a decrease in myosin heavy chain (MHC) IIb, an increase in MHCIIa+d/x, and new expression of MHCI. In the FK506-administered group, however, overload-induced muscle fiber-type transition was completely prevented. We have demonstrated, therefore, that the CaN signaling pathway is required for fast-to-slow skeletal muscle fiber-type transition. Furthermore, we also confirmed that the protein expression levels of downstream effectors of CaN signaling exhibit a transient increase in the early phase of the overloaded condition.

6

Protein kinase inhibitors as antileukemic agents

75%

Fabbro D.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2A

7

Hippo pathway - brief overview of its relevance in cancer

63%

Zygulska A. L. , Krzemieniecki K. , Pierzchalski P.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 3

8

Interplay of two PP2A B subunits in high light acclimation

63%

Konert G. , Trotta A. , Rahikainen M. , Kangasjarvi S.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

9

A proliferation-inducing ligand regulation in polymorphonuclear neutrophils by Panax ginseng

63%

Ratajczak-Wrona W. , Wawrusiewicz-Kurylonek N. , Garley M. , Kretowski A. J. , Jablonska E.

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2020

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tom 68

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nr 6

10

The effect of buffalo CD14 shRNA on the gene expression of TLR4 signal pathway in buffalo monocyte/macrophages

63%

Li X. , Li M. , Huang S. , Qiao S. , Qin Z. , Kang C. , Shi D.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 4

EN

CD14 plays a crucial role in the inflammatory response to lipopolysaccharide (LPS), which interacts with TLR4 and MD-2 to enable cell activation, resulting in inflammation. Upstream inhibition of the inflammation pathway mediated by bacterial LPS, toll-like receptor 4 (TLR4) and cluster of differentiation antigen 14 (CD14) was proven to be an effective therapeutic approach for attenuating harmful immune activation. To explore the effect of CD14 downregulation on the expression of TLR4 signaling pathway-related genes after LPS stimulation in buffalo (Bubalus bubalis) monocyte/macrophages, effective CD14 shRNA sequences were screened using qRT-PCR and FACS analysis with buffalo CD14 shRNA lentiviral recombinant plasmids (pSicoRGFP-shRNA) and buffalo CD14 fusion expression plasmids (pDsRed-N1-buffalo CD14) co-transfected into HEK293T cells via liposomes. Of the tested shRNAs, shRNA-1041 revealed the highest knockdown efficiency (p < 0.01). When buffalo peripheral blood monocyte/macrophages were infected with shRNA-1041 lentivirus and stimulated with LPS, the expression of endogenous CD14 was significantly decreased by CD14 shRNA (p < 0.01), and the mRNA expression levels of TLR4, IL-6 and TNF-α were also significantly downregulated compared to the control groups (p < 0.01). These results demonstrated that the knockdown of endogenous CD14 had clear regulatory effects on the signal transduction of TLR4 after stimulation with LPS. These results may provide a better understanding of the molecular mechanisms of CD14 regulation in the development of several buffalo diseases.

11

CRK5 as a convergence node between senescence and abiotic stress responses

63%

Burdniak P. , Glow D. , Rusaczonek A. , Karpinski S.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

12

ZNF750 inhibits the proliferation and invasion of melanoma cells through modulating the Wnt/beta-catenin signaling pathway

63%

Du Y. , LV G. , Jing C. , Liu J. , Liu J.

Folia Histochemica et Cytobiologica

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2020

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tom 58

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nr 4

13

Degenerate specificity of PDZ domains from RhoA-specific nucleotide exchange factors PDZRhoGEF and LARG

63%

Smietana K. , Kasztura M. , Paduch M. , Derewenda U. , Derewenda Z. S. , Otlewski J.

Acta Biochimica Polonica

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2008

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tom 55

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nr 2

269-280

EN

PDZ domains are ubiquitous protein–protein interaction modules which bind short, usually carboxyterminal fragments of receptors, other integral or membrane-associated proteins, and occasionally cytosolic proteins. Their role in organizing multiprotein complexes at the cellular membrane is crucial for many signaling pathways, but the rules defining their binding specificity are still poorly understood and do not readily explain the observed diversity of their known binding partners. Two homologous RhoA-specific, multidomain nucleotide exchange factors PDZRhoGEF and LARG contain PDZ domains which show a particularly broad recognition profile, as suggested by the identification of five diverse biological targets. To investigate the molecular roots of this phenomenon, we constructed a phage display library of random carboxyterminal hexapeptides. Peptide variants corresponding to the sequences identified in library selection were synthesized and their affinities for both PDZ domains were measured and compared with those of peptides derived from sequences of natural partners. Based on the analysis of the binding sequences identified for PDZRhoGEF, we propose a sequence for an ‘optimal’ binding partner. Our results support the hypothesis that PDZ–peptide interactions may be best understood when one considers the sum of entropic and dynamic effects for each peptide as a whole entity, rather than preferences for specific residues at a given position.

14

Mechanisms of vasorelaxation induced by total flavonoids of Euphorbia humifusa in rat aorta

63%

Wang T. T. , Zhou Z. Q. , Wang S. , Ji X. W. , Wu B. , Sun L. Y. , Wen J. F. , Kang D. G. , Lee H. S. , Cho K. W. , Jin S. N.

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nr 4

15

NO-induced alleviation of dormancy of apple embryos is related to modification of phytohormonal signaling pathways

63%

Andryka P. , Wisniewska A. , Gniazdowska A. , Krasuska U. , Bogatek R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

16

TLR4 ligation induces expression of APRIL molecule in human neutrophils — a preliminary study

63%

Jablonska E. , Wawrusiewicz-Kurylonek N. , Garley M. , Kretowski A.

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tom 50

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nr 2

17

Sensitivity analysis of mathematical models of signalling pathways

63%

Charzynska A. , Nalecz A. , Rybinski M. , Gambin A.

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2012

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tom 93

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nr 3

18

Echinacea purpurea extract (cichoric acid) exerts an anti-inflammatory effect on yak PBMCs and regulates the TLR4 signalling pathway

63%

Xue C.-H. , A S.-X. , Wang M.-J. , Wu Q. , Liu J.-H. , Zhang L.-F. , Wu Y. , Wu H. , Chai S.-T.

Journal of Veterinary Research

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2021

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tom 65

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nr 1

19

WNT7A expression is downregulated in T lymphocytes after T-cell receptor activation due to histone modifications and in T-ALL by DNA methylation

63%

Barreto-Vargas C. , Alvarez-Zavala M. , Garcia-Chagollan M. , Hernandez-Flores G. , Aguilar-Lemarroy A. , Jave-Suarez L. F.

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2020

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tom 68

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nr 3

20

Components of the IFN-gamma signalling pathway in tumorigenesis

63%

Blanck G.

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nr 3