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Micropropagation of Chamaedaphne calyculata (L.) Moench by direct shoot organogenesis

100%

Zrobek-Sokolnik A. , Slipiko A. , Kucewicz M. , Milewicz M. , Holdynski C.

Polish Journal of Natural Sciences

2011

tom 26

nr 3

The objective of this experiment was to investigate the effect of various concentrations of sucrose, 6-(γ,γ-dimethylallylamino)-purine (2iP) and pH values of Lloyd’s and McCown’s medium (1981, WPM) on the induction of lateral shoot growth in Chamaedaphne calyculata (L.) Moench. The explants were 2–3 cm nodal sections without the apex, with preserved leaves, from plants grown in vitro. The highest regenerative capacity was observed in culture media without cytokinin, with 58 mM sucrose content and pH 5.0. The lowest capacity for shoot organogenesis was reported in media with pH 5.6 with a higher sucrose content (88 mM) and 25 μM of 2iP. 80% of rooted explants were successfully transferred to ex vitro conditions. The survival rate of plantlets reached around 60% after three months of greenhouse cultivation.

Badania podjęte w tej pracy miały na celu zbadanie wpływu różnych stężeń sacharozy, 6-(γ,γ-dimetyloallioamino)-puryny (2iP) oraz odczynu pH pożywki LLOYDA i MCCOWNA (1981,WPM) na efektywność procesu indukcji rozwoju pędów bocznych u Chamaedaphne calyculata (L.) Moench. Eksplantatami były fragmenty węzłowe pozbawione wierzchołka wzrostu pędu o długości od 2 do 3 cm, z zachowanymi liśćmi, pochodzące z roślin hodowlanych w kulturach in vitro. Najwyższy stopień regeneracji stwierdzono na podłożu bez dodatku cytokininy, zawierającym sacharozę w stężeniu 58 mM, o pH 5,0. Najsłabszą organogenezę pędową obserwowano na pożywkach o wyższym stężeniu sacharozy (88 mM), z 2iP o stężeniu 25 μM oraz o pH 5,6. 80% ukorzenionych eksplantatów pomyślnie przeniesiono do warunków ex vitro. Przeżywalność roślinek w warunkach szklarniowych po 3 miesiącach wynosiła około 60%.

High-frequency adventitious shoot organogenesis from in vitro stem explants of Scutellaria araxensis Grossh

86%

Gharari Z. , Bagheri K. , Sharari A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2022

tom 103

nr 2

Structural diversity of Papaver somniferum L. cell surfaces in vitro depending on particular steps of plant regeneration and morphogenetic program

72%

Ovecka M. , Bobak M.

Acta Physiologiae Plantarum

1999

tom 21

nr 2

Culture of Papaver somniferum in vitro was used for a characterisation of cell surface structures and mode of cell adhesion and cell separation during cell differentiation and plant regeneration in somatic embryogenesis and shoot organogenesis. In early stages of somatic embryogenesis, cell type-specific and developmentally regulated change of cell morphogenesis was demonstrated. Cell wall of separated embryonic cells were self-covered with external tubular network, whereas morphogenetic co-ordination of adhered cells of somatic proembryos was supported by fine and fibrillar external cell wall continuum of peripheral cells, interconnecting also local sites of cell separation. Such type of cell contacts disappeared during histogenesis, when the protodermis formation took place. Tight cell adhesion of activated cells with polar cell wall thickening, and production of extent mucilage on the periphery were the crucial aspects of meristemoids. Fine amorphous layer covered developing shoot primordia, but we have not observed such comparable external fibrillar network. On the contrary intercellular separation of differentiated cells in regenerated organs, and accepting distinct developmental system of somatic embryogenesis and shoot organogenesis, cell adhesion in early stages and ultrastructural changes associated with tissue disorganisation, and the subsequent reorganisation into either embryos or shoots appear to be regulatory morphogenetical events of plant regeneration in vitro.