Wyniki wyszukiwania - Biblioteka Nauki

1

Association between a biomarker of exposure to polycyclic aromatic hydrocarbons and semen quality

100%

Jurewicz J. , Radwan M. , Sobala W. , Brzeźnicki S. , Ligocka D. , Radwan P. , Bochenek M. , Hanke W.

International Journal of Occupational Medicine and Environmental Health

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2013

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tom 26

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nr 5

790-801

EN

Objectives: Growing evidence supports the reproductive and developmental toxicity of polycyclic aromatic hydrocarbons (PAHs) from prenatal and postnatal exposure, but the results of epidemiological studies regarding harmful effects of PAHs exposure on male reproductive system still remain limited and inconclusive. The aim of the present study was to investigate the relationship between 1-hydroxypyrene, a biomarker of polycyclic aromatic hydrocarbons exposure and semen quality. Materials and Methods: The study population consisted of 277 men attending an infertility clinic for diagnostic purposes and having normal semen concentration of 20-300 mln/ml or slight oligozoospermia (semen concentration: 15-20 mln/ml) (WHO 1999). All the men were healthy and under 45 years of age. All participants were interviewed and provided a semen sample. The interview included questions concerning demographics, socio-economic status, medical history related to past diseases which may have an impact on semen quality, lifestyle factors and occupational information. Concentrations of 1-hydroxypyrene (1-OHP) in the urine samples were analyzed using high performance liquid chromatography (HPLC). Results: A positive association was found between the level of 1-OHP in urine and sperm neck abnormalities as well as the percentage of static sperm cells (p = 0.001, p = 0.018, respectively). Additionally, exposure to PAHs measured by 1-OHP in urine decreased semen volume and the percentage of motile sperm cells (p = 0.014, p = 0.0001, respectively). Conclusions: Presented findings indicate that the environmental level of PAHs exposure adversely affects male semen quality. The future large-scale studies should incorporate different biomarkers to generate a more accurate and full assessment of the effects of PAHs exposure on male fertility.

2

Are semen quality parameters sufficient for biomonitoring spermatozoa DNA integrity and oxidatively damaged DNA

88%

Jeng H. A. , Li R.-N. , Lin W.-Y.

Biomonitoring

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2015

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tom 2

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nr 1

EN

The present study aimed to investigate the relationship between semen quality parameters and DNA integrity, and determine whether semen quality parameters could serve as a reliable biomarker for monitoring sperm DNA damage. Conventional semen parameters from a total of 202 male human subjects were analyzed. DNA fragmentation and 8-oxo-7,8-dihydro-2′- deoxyguanosine (8-oxoGuo) were used to assess sperm DNA integrity. DNA fragmentation was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and sperm chromatin structure assay (SCSA), while 8-oxodGuo was quantified by the liquid chromatography/tandem mass spectrometry (LC-MS/MS) coupled with an on-line solid phase system. The levels of 8-oxodGuo levels in sperm were related to the percentages of DNA fragmentation measured by both the TUNEL and SCSA (r = 0.22, p = 0.048; r = 0.12, p = 0.039). Sperm vitality, motility and morphology from all of the participants exhibited a weak correlation with the levels of 8-oxodGuo and the percentages of DNA fragmentation. Semen quality parameters may be independent of the formation of DNA fragmentation and oxidative adducts in sperm. Semen quality parameters may be insufficient to monitor sperm DNA fragmentation and oxidative damage. DNA damage in sperm is recommended to be included in routine measurements.

3

Environmental exposure to non-persistent endocrine disrupting chemicals and semen quality: An overview of the current epidemiological evidence

75%

Zamkowska D. , Karwacka A. , Jurewicz J. , Radwan M.

International Journal of Occupational Medicine and Environmental Health

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2018

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tom 31

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nr 4

377-414

EN

Some of the recent publications have reported a decline in semen quality in the last few decades. This phenomenon is associated with environmental factors, particularly with exposure to endocrine disrupting chemicals (EDCs). The aim of this publication is to critically review the literature on exposure to the following 6 ubiquitous environmental non-persistent EDCs: bisphenol A, triclosan, parabens, synthetic pyrethroids, organophosphate pesticides and phthalates, and on their influence on semen quality measured as sperm concentration, sperm volume, total sperm count, motility, total motile count, morphology, sperm motion, sperm DNA damage (comet extent, tail length, tail distributed moment, percent of DNA located in the tail (tail%), DNA fragmentation index, high DNA stainability, X:Y ratio and aneuploidy. Several electronic databases were systematically searched until 31 August 2016. Studies were qualified for the review if they: linked environmental exposure to non-persistent EDCs to semen quality outcomes, were published in English after 2006 (and, in the case of phthalates, if they were published after 2009) and were conducted in the case of humans. Out of the 970 references, 45 articles were included in the review. This review adds to the body of evidence that exposure to non-persistent EDCs may affect semen quality parameters and decrease semen quality. Int J Occup Med Environ Health 2018;31(4):377–414

4

Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons

75%

Jeng H. A. , Pan C.-H. , Chao M.-R. , Chiu C.-C. , Zhou G. , Chou C.-K. , Lin W.-Y.

International Journal of Occupational Medicine and Environmental Health

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2016

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tom 29

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nr 6

915-926

EN

Objectives The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. Material and methods The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo). A questionnaire was conducted to collect demographic and potential confounding data. Results The coke oven workers had lower percentages of sperm motility, vitality and normal morphology than the control group, but the difference was not significant. For DNA integrity, the coke oven workers had significantly higher concentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048, respectively). However, DNA fragmentation percentages did not significantly increase as compared to those in the subjects from the control group (p = 0.232). There was no correlation between sperm quality parameters and DNA integrity indicators. Conclusions Occupational exposure of the coke oven workers to PAHs was associated with decreased sperm DNA integrity. Int J Occup Med Environ Health 2016;29(6):915–926

5

Iron-induced luminescence of boar spermatozoa cells

75%

Gogol P.

Annals of Animal Science

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2005

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tom 05

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nr 1

6

The effect of different thermal conditions on drone semen quality and number of spermatozoa entering the spermatheca of queen bee

75%

Bienkowska M. , Panasiuk B. , Wegrzynowicz P. , Gerula D.

Journal of Apicultural Science

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2011

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tom 55

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nr 2

7

Assessment of the morphometry of heads of normal sperm and sperm with the Dag defect in the semen of Duroc boars

75%

Banaszewska D. , Andraszek K.

Journal of Veterinary Research

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2021

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tom 65

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nr 2

8

The effect of light source on characteristics of Japanese quail [Coturnix japonica] semen

75%

Gwara T. , Walczak M. , Lukaszewicz E. , Chelmonska B.

Zeszyty Naukowe Akademii Rolniczej we Wrocławiu. Zootechnika

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2004

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tom 50

9

Effects of administration of Escherichia coli lipopolysaccharides and flunixin meglumine on semen quality in the stallion

75%

Danek J.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 4

10

Relationship between semen parameters, fertility and hatchability of eggs in five original laying strains of hens

75%

Machal L. , Jankowski J.

Polish Journal of Natural Sciences

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2002

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tom 10

11

Preliminary study of chinchilla [Chinchilla velligera] semen quality in the context of its practical employment in selection

75%

Szatkowska I. , Sulik M. , Udala J.

Zeszyty Naukowe. Przegląd Hodowlany

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1996

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tom 27

12

The effect of the GnRH on semen quality of the Polish longwool sheep rams exposed to different light conditions

75%

Udala J.

Advances in Agricultural Sciences

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1993

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tom 02

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nr 1

13

Semen quality parameters in outbred male mice from four different selected lines

63%

Sokolowski G. , Strzalkowska A. , Swiderek W. , Fiszdon K. , Gajewska M.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2013

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tom 52

EN

Breeding of (outbred) selective lines of laboratory mouse was initiated in Warsaw University of Life Sciences about 40 years ago. It bred Heavy (C) and Light (L) mice selected opposite for body weight at weaning (21st day of life), S mice line selected for higher testes weight, and control (K) mice without selection. All lines have identical genetic background, but different directions of selections caused diversification of specific phe-notypic traits between them. The purpose of this study was to compare semen quantity and quality parameters in outbred C, K, L and S male mice in the context of measurements of average body and testes weight for each line. Research materials were seminal fluids squeezed out of the vas deferens from 20 outbred C, K, L and S male mice (5 males per group). Animals had been euthanized, and necropsy was performed. Body and testes weight was measured. Also sperm concentration, viability (by Eosin test), cytoplasmic membrane integrity degree (HOS test), sperm head morphology and maturity were estimated. It was shown that S male mice, which have much higher testes weight, also have a significant increase of viable spermatozoa according to control line. Moreover, sperm concentration from S males is at least two times higher than in other selective lines.

PL

Parametry jakości plemników samców myszy z czterech outbredowych linii selekcyjnych. Celem przeprowadzonych badań było dokonanie oceny jakości parametrów plemników nasieniowodowych, pobranych od 20 samców (po 5 samców z linii) myszy z linii selekcjonowanych przez wiele pokoleń: przeciwstawnie na masę ciała (C i L), masę jąder (S) oraz samców stanowiących linię kontrolną (K), w kontekście pomiarów średnich mas ciała i jąder dla poszczególnych linii. Materiał badawczy stanowiły plemniki pobrane z nasieniowodów od zwierząt poselekcyjnych. Oszacowano liczbę plemników w 1 ml pożywki (M2). Dokonano analizy parametrów jakości plemników, wykonując test oceny żywotności plemników, test położenia kropli cytoplazmatycznej, który jest miarą dojrzałości plemników oraz test hipoosmotyczny (HOS) do oceny integralności błony cytoplazmatycznej witek plemników. Ponadto dokonano oceny morfologii główek plemników. Wykazano, że samce linii S w porównaniu z osobnikami z linii kontrolnej K oraz linii ciężkiej (C) i lekkiej (L) mają istotnie większą masę jąder, większy odsetek dojrzałych i żywotnych plemników, a także 2-4-krotnie większą koncentrację plemników liczoną w 1 ml medium.

14

Morphological changes in wild boar (Sus scrofa L.) semen in annual cycle

63%

Kozdrowski R. , Dubiel A.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2004

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tom 07

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nr 2

15

Cryopreservation of bull semen in OptiXcell and conventional extenders: Comparison of semen quality and fertility

63%

Ansari M. S. , Rakha B. A. , Akhter S.

Animal Science Papers and Reports

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2017

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tom 35

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nr 3

EN

Commercially available OptiXcell® extender was compared with conventional extenders for freezability and in vivo fertility of bull semen. Semen was collected from three Friesian bulls for five weeks (replicate) and qualifying ejaculates (motility >60%, concentration >0.5 billion/mL, volume >lmL) were diluted (37°C; 50 x 10⁶ spermatozoa/ml) with OptiXcell®, tris-citric egg yolk and egg yolk-citrate extenders. Diluted semen was cooled to 4°C in 2 hours, equilibrated for 4 hours and filled in 0.5 ml straws. The straws were kept over liquid nitrogen vapours for 10 minutes and plunged into liquid nitrogen. Percentages of post thaw sperm motility and plasma membrane integrity were recorded higher (P<0.05) in OptiXcell® compared to tris-citric egg yolk followed by egg yolk-citrate extender. Sperm viability (%) were recorded higher (P<0.05) in OptiXcell® compared to tris-citric egg yolk and egg yolk citrate extender. Percentages of normal apical ridge and DNA integrity were higher (P<0.05) in OptiXcell® and tris-citric egg yolk extender compared to egg yolk-citrate extender. Higher (P<0.05) fertility rate was recorded with semen frozen in OptiXcell® compared to triscitric egg yolk and egg yolk-citrate extender. In conclusion, OptiXcell® is superior to conventional extenders for spermatozoa) quality of frozen-thawed bull semen and produced higher fertility rates under field conditions.

16

Effect of L-cysteine in extender on post-thaw quality of Sahiwal bull semen

63%

Ansari M. S. , Rakha B. A. , Akhter S.

Animal Science Papers and Reports

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2011

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tom 29

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nr 3

EN

The study was conducted to evaluate the effect of addition of L-cysteine to tris-citric acid (TCA) extender on the post-thaw quality of Sahiwal bull semen. For this purpose, two consecutive ejaculates were collected from three Sahiwal bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in TCA extender having L-cysteine either 0.0 (control) or 0.5, 1.0 or 2.0 mM. Diluted semen was cooled to 4°C for 2 h, equilibrated for 4 h at 4°C, filled in straws at 4°C, kept in liquid nitrogen vapours for 10 min and then stored in the liquid nitrogen. Thawing was performed after 24 h of storage, at 37°C for 30 s. and the sperm motility, viability, plasma membrane and acrosomal integrity were assessed. Higher (P<0.05) sperm motility, viability, plasma membrane and acrosomal integrity were observed using extenders containing 1.0 or 2.0 mM compared to those containing 0.5 or 0.0 mM L-cysteine. It is concluded that addition of L-cysteine (to reach 1.0-2.0 mM) in TCA extender improves the post-thaw quality of Sahiwal bull semen.

17

Examination of sperm chromatin structure and fertility of boar semen

63%

Bochenek M. , Smorag Z. , Rozycki M. , Dziadek K.

Roczniki Naukowe Zootechniki

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2000

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tom 27

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nr 2

18

Comparison of in vitro methods for evaluation of boar semen quality

63%

Serniene L. , Riskeviciene V. , Banys A. , Zilinskas H.

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tom 61

|

nr 03

19

The effect of environment during gander semen collection on spermatozoa quality and freezability

63%

Lukaszewicz E.

Zeszyty Naukowe Akademii Rolniczej we Wrocławiu. Zootechnika

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2004

|

tom 50

20

Correlation between zinc, copper, and calcium concentration in seminal plasma and the characteristics of semen in relation to breed and age of stallions

63%

Danek J. , Wisniewski E. , Krumrych W.

Bulletin of the Veterinary Institute in Puławy

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1998

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tom 42

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nr 2

EN

This work aimed to establish correlation between concentration of zinc, copper and calcium in seminal plasma and characteristics of semen of stallions of various breeds (Thoroughbred -TB, Great Polish Horses -GPH, Polish Primitive Horses -PPH) and aged (≤ 11 years, > 11 years, > 14 years ). Measurements revealed interbreed differences in the concentration of zinc (TB:GPH, TB:PPH), copper (PPH:TB) and calcium (GPH:TB) in the seminal plasma of stallions. No age-dependent alterations in the element concentrations of plasma were found in stallions. There was a significantly (P<0.05) positive correlation between zinc and copper concentrations in the seminal plasma (PPH) and a positive correlation between selected characteristics of stallion semen, especially between zinc and concentration of spermatozoa, (GPH, PPH, stallions ≤ 11 years of age, > 11 years, all stallions), between calcium and spermatozoa with secondary changes (GPH, stallions ≤ 11 years of age, all stallions) and also a negative correlation between copper concentration and motile- and live spermatozoa (TB, GPH, stallions > 11 years of age, all stallions).