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EN
The Badenian (Middle Miocene) evaporite deposits of the Carpathian Foredeep in Poland, Ukraine and Czech Republic, contain large bottom-grown primary gypsum crystals (selenite) which are some of the largest in the world. The 0.5-3.5 m long crystals are arranged in a palisade manner and create specific intergrowths similar to the contact swallow-tail {101} twins known in other areas. They occur in one stratigraphical interval that is several metres thick. The largest specimens were found near Busko in Southern Poland. The selenite crystals are commonly 1-1.5 m long, but specimens exceeding 2.5 m in length are present but are rare and poorly documented. Some years ago one specimen approaching 3.5 m in length was recognized at Bogucice-Skałki and seems to be the largest known and existing mineral crystal in Poland. Recently another ca. 3.5 m long selenite specimen was exposed at nearby Gacki. Both these crystals are partly damaged, and one is not fully exposed, and therefore it is difficult to establish which is (or was) actually the largest one.
EN
Arsenic is a worldwide environmental pollutant that generates public health concerns. Various types of cancers and other diseases, including neurological disorders, have been associated with human consumption of arsenic in drinking water. At the molecular level, arsenic and its metabolites have the capacity to provoke genome instability, causing altered expression of genes. One such target of arsenic is the Pax6 gene that encodes a transcription factor in neuronal cells. The aim of this study was to evaluate the effect of two antioxidants, α-tocopheryl succinate (α-TOS) and sodium selenite, on Pax6 gene expression levels in the forebrain and cerebellum of Golden Syrian hamsters chronically exposed to arsenic in drinking water. Animals were divided into six groups. Using quantitative real-time reverse transcriptase (RT)-PCR analysis, we confirmed that arsenic downregulates Pax6 expression in nervous tissues by 53 ± 21% and 32 ± 7% in the forebrain and cerebellum, respectively. In the presence of arsenic, treatment with α-TOS did not modify Pax6 expression in nervous tissues; however, sodium selenite completely restored Pax6 expression in the arsenic-exposed hamster forebrain, but not the cerebellum. Although our results suggest the use of selenite to restore the expression of a neuronal gene in arsenic-exposed animals, its use and efficacy in the human population require further studies.
EN
To elucidate the molecular mechanisms of the protective action of stigmatellin (an inhibitor of complex III of mitochondrial electron transport chain, mtETC) against the heavy metal-induced cytotoxicity, we tested its effectiveness against mitochondrial membrane permeabilization produced by heavy metal ions Cd2+, Hg2+, Cu2+ and Zn2+, as well as by Ca2+ (in the presence of Pi) or Se (in form of Na2SeO3) using isolated rat liver mitochondria. It was shown that stigmatellin modulated mitochondrial swelling produced by these metals/metalloids in the isotonic sucrose medium in the presence of ascorbate plus tetramethyl-p-phenylenediamine (complex IV substrates added for energization of the mitochondria). It was found that stigmatellin and other mtETC inhibitors enhanced the mitochondrial swelling induced by selenite. However, in the same medium, all the mtETC inhibitors tested as well as cyclosporin A and bongkrekic acid did not significantly affect Cu2+-induced swelling. In contrast, the high-amplitude swelling produced by Cd2+, Hg2+, Zn2+, or Ca2+ plus Pi was significantly depressed by these inhibitors. Significant differences in the action of these metals/metalloids on the redox status of pyridine nucleotides, transmembrane potential and mitochondrial respiration were also observed. In the light of these results as well as the data from the recent literature, our hypothesis on a possible involvement of the respiratory supercomplex, formed by complex I (P-site) and complex III (S-site) in the mitochondrial permeabilization mediated by the mitochondrial transition pore, is updated.
EN
The influence of dietary conjugated linoleic acid (CLA) isomers (CLAmix) and/or Se as Na2SeO3 (SeIV) or selenized yeast (SeY) on the relative body weight gain (RBWG) of rats, pancreas weight, concentrations of CLA isomers and of other fatty acids (FA) in the pancreas were studied. For 6 weeks, female Wistar rats (aged 8 weeks) were fed ad libitum the Labofeed H diet enriched in 1.5% CLAmix, 0.2 ppm Se (LSe), 0.5 ppm Se (HSe) as SeIV (i.e. LSeIV or HSeIV, respectively), or SeY (i.e. LSeY or HSeY, respectively). Each group contained eight rats. The results documented that dietary LSeIV and CLAmix most effectively increased the RBWG of rats and feed conversion efficiency, while the diet with HSeIV most efficiently increased pancreas weight compared with the control rats. The diets enriched in CLAmix and Se as SeIV or SeY increased the sum of saturated fatty acids (SFA), the atherogenic SFA, A-SFA index, thrombogenic SFA (T-SFA), and the T-SFA index in the pancreas of rats compared with the control group. The diets containing CLAmix and Se as SeIV or SeY stimulated the accumulation of cis9trans11CLA, trans10cis12CLA, as well as the sum of all CLA isomers in the pancreas compared with rats fed the diet enriched in CLAmix. These diets increased the concentration of n-6 polyunsaturated FA (n-6 PUFA) and decreased the level of long-chain n-6 PUFA (Ln-6 PUFA) in the pancreas compared with the control rats and rats fed the diets with SeIV or SeY. The diets containing CLAmix and Se (as SeIV or SeY) increased the concentration of n-3 PUFA and Ln-3 PUFA in the pancreas compared with the control rats. Our study findings documenting that dietary Se and/or CLAmix and Se (as SeIV or SeY) considerably increased the concentration of PUFA, especially n-3 PUFA, improved the n-6 PUFA/n-3 PUFA ratio in the pancreas are valuable information for scientists carrying out research to improve animal and human health.
EN
We investigated the effect of selenium form and dose on the total glutathione and non-protein -SH group contents in the edible spinach (Spinacia oleracea L.) and ground tomato (Lycopersicon esculentum Mill.) plants. Our experiments were carried out in a hydroponic culture. Selenium was added to the culture medium in its selenite (Na2SeO3 x 5H2O) and selenate (Na2SeO4) forms. Regardless of the selenium form, we observed an increase in the non-protein thiol content. The non-protein -SH group content depended on the form and dose of selenium as well as on the organ and plant species. Regardless of the selenium form, a higher content of non-protein -SH groups were found in the spinach biomass than in the tomato biomass. Selenite contributed to a larger accumulation of non-protein -SH groups in the roots, whereas selenate contributed to their accumulation in the shoots
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Content available remote O gipsie inaczej
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PL
Gips, jeden z podstawowych materiałów wiążących, stanowi przedmiot zainteresowania nie tylko specjalistów z różnych dziedzin nauk technicznych i przyrodniczych, ale również artystów. Opracowanie poświęcone jest historii gipsu i jego roli cywilizacyjnej. Omówiono szereg przykładów wykorzystania szlachetnych odmian gipsu - alabastru i selenitu. Praca zawiera również przykłady wykorzystania gipsu przez artystów jako tworzywa w sensie dosłownym oraz jako tematu ich dzieł. W pracy podkreślono, że gips ma nie tylko piękną historię, ale również duże perspektywy rozwojowe.
EN
Gypsum, one of the basic binders, is not only the subject of interest of the specialists from different branches of science and technology, but also attracts the artists. The presented paper is devoted to the history of gypsum and the role of gypsum as a matter stimulating the development of civilisation. The examples of the presence of noble varieties of gypsum - alabaster and selenite in art and decoration are shown. The use of gypsum as a materials and as an abstractive motive in literature is also highlighted. Not only an interesting history but also the promising prospects of gypsum have been underlined in this work.
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EN
Effect of three cadmium compounds (CdSeO4, CdSeO3 and Cd(NCSe)2(nia)2) containing Se in different oxidation states were used for experiments. Pea seedlings (cv. Felix) cultivated in hydroponic solutions under control conditions were treated with the above-mentioned compounds for two weeks (c = 3÷120 µmol dm-3). Then root and shoot dry mass was estimated and the concentrations of Cd and Se in plant organs were determined using AAS. Bioaccumulation factors related to both investigated elements (Cd, Se) as well as translocation factors were evaluated. Higher compound concentrations were toxic and desiccation of the shoots was observed. Cd concentration in the roots reached higher levels than in the shoots and accumulated Cd amount in plant organs increased with increasing Cd concentration. Whereas treatment with CdSeO3 and Cd(NCSe)2(nia)2) resulted in expressively higher Se concentration in roots, for CdSeO4 treatment Se concentration in shoots exceeded Se concentration in the roots. Therefore treatment with CdSeO4resulted in much higher mobility of Cd and Se within the plants than the treatment with CdSeO3 and Cd(NCSe)2(nia)2).
PL
W eksperymencie wykorzystano trzy związki kadmu (CdSeO4, CdSeO3, Cd(NCSe)2(nia)2) zawierające Se w rożnych stopniach utlenienia. Wschody grochu uprawiane w wodzie w warunkach laboratoryjnych były traktowane wymienionymi związkami przez dwa tygodnie (c = 3÷120 µmol-dm-3). Następnie w próbkach korzeni i pędów roślin oznaczano stężenia Cd i Se z pomocą AAS. Dokonano oceny bioakumulacji i czynników wpływających na przemieszczanie się badanych pierwiastków. Większe stężenie związków były toksyczne i powodowały wysuszenie pędów roślin. Stężenia Cd w korzeniach były większe niż w pędach roślin, a stężenie zakumulowanego Cd w organach rośliny zwiększało się wraz ze wzrostem stężenia Cd. Podczas działania CdSeO3, Cd(NCSe)2(nia)2wyniki wyraźnie wskazywały, że stężenie Se w korzeniach wzrosło, podczas działania związkiem CdSeO4 stężenie Se w pędach było większe od stężenia Se w korzeniach. Zatem w wyniku działania CdSeO4 ma miejsce większa kumulacja Cd i Se w roślinie niż podczas działania związkami CdSeO3 i CdSeO3, Cd(NCSe)2(nia)2.
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tom Vol. 43, Nr 4
405-428
EN
Seven facies (five primary and two diagenetic) and 12 subfacies are distinguished within the Nida Gypsum deposits which are a part of the widespread Middle Miocene (Badenian) evaporites of the Carpathian Foredeep cropping out in vicinity of Busko in southern Poland. Facies are defined as products of specific mechanisms of evaporitic deposition: syntaxial bottom growth of gypsum crystals, microbial gypsum deposition (mainly gypsification of organic mats), mechanical deposition and diagenetic and weathering processes. Primary facies and subfacies, and their uncommon sedimentary structures (such as: up to 3.5 m high bottom-grown gypsum crystals, several metres high selenitic domes, gypsum stromatolite domes, halite-solution collapse breccias) record a varied shallow water (0-5 m) evaporitic environment, controlled mainly by depth, salinity and climate.
PL
W badeńskich gipsach Ponidzia wyróżniono 6 facji siarczanowych: gipsy szklicowe, rumosze kryształów gipsu, gipsy trawiaste, szablaste, mikrokrystaliczne, porfiroblastyczne i jedna fację węglanową. W obrębie 5 pierwszych facji wyróżniono 12 subfacji i scharakteryzowano środowiska ich sedymentacji, które w większości są typowe dla płytkiego, okresowo wynurzanego zbiornika ewaporacyjnego. Facje zdefiniowano jako produkty kilku podstawowych mechanizmów depozycyjnych (por. E.Mutti, F.Ricci Lucchi, 1975), m.in. takich jak: (I) syntaksjalny wzrost dużych kryształów gipsu wprost na dnie basenu (gipsy szklicowe, trawiaste i szablaste), (II) mikrobialną (sensu R.V.Burne, L.S.Moore, 1987) depozycję drobnokrystalicznego gipsu, głównie poprzez gipsyfikację mat organicznych (gipsy trawiaste), (III) depozycję mechaniczną (gipsy mikrokrystaliczne), obejmującą opadanie i osiadanie drobnych kryształów gipsu wytrąconych w tonu wodnej, oraz redepozycję osadu gipsowego. Zróżnicowanie facjalne gipsów Ponidzia wynika przede wszystkim z wahań zasolenia i głębokości basenu oraz wilgotności klimatu.
PL
W wątrobie, nerce i mózgowiu szczurów otrzymujących przez 12 tyg. wodę zawierającą 50 µg Cd2+ /cm3 i/lub dożołądkowo 5 µg Se/100 g masy ciała, badano aktywność dehydrogenazy glutaminianowej (GLDH), arginazy (ArgE), katalazy (CAT) oraz całkowitą zawartość wolnych aminokwasów (AmAc) i mocznika (Ur). Zatruwanie kadmem powodowało wzrost aktywności GLDH i ArgE w nerce i mózgowiu oraz zawartości mocznika w badanych narządach. Podawanie seleninu obniżało o kilkanaście procent zawartość Ur w nerce oraz aktywność CAT w wątrobie i nerce, nie wpływając na inne wskaźniki. Selen przeciwdziałał zmianom wywoływanym przez kadm z wyjątkiem zmian zawartości Ur w tkankach. Zawartość AmAc w narządach nie była zmieniana przez badane czynniki.
EN
Cadmium has been reported as factor affecting metabolism of proteins and amino acids in animal organs, while selenium compounds are known to counteract some toxic effects of cadmium. Moreover, the metabolism of ingested selenium is related to the processes involving cysteine, methionine and glutathione. These effects were studied on male Wistar rats which received for 12 weeks tap water containing 50 µg/ml Cd (II), and/or were given twice a week 5 µg selenium (as seleni te) per 100 g of body weight. Activity of glutamate dehydrogenase (GLDH), arginase (ArgE) and the content of free amino acids (AmAc) and urea (Ur) were determined in crude homogenates and deproteinized liver, kidney, and brain supernatants. Since amino acid oxidation was accompanied by generation of hydrogen peroxide, the activity of catalase (CAT) was also determined. Cadmium increased renal and cerebral GLDH and ArgE activity as well as urea content of the examined organs. Selenite alone did not affect these parameters except for decreased urea content in the brain and kidney, while in the cadmium-treated rats selenite prevented most of the cadmium effects. Total content of free AA in the organs was not altered by the experimental factors. CAT activity in kidney was enhanced following cadmium treatment, but this effect was reversed by the concomitant treatment with selenium. Selenium gavaged to the rats not exposed to cadmium reduced CAT activity in the liver and kidney. Our results suggest that subchronic cadmium ingestion enhances protein and amino acid catabolism, while increased selenium intake prevents cadmium - induced effects of amino acid metabolism.
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