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EN
Liparis loeselii (L.) Rich. is an endangered orchid species which occurs in wetland habitats. For successful conservation of this orchid it is necessary to recognize its adaptive traits and special habitat requirements. Therefore, we examined morphological and anatomical traits in relation to the water level, cover of vascular plants and seed viability of L. loeselii in habitats with different water level fluctuations. Field studies of L. loeselii were carried out in nine localities and four habitat types: alkaline fen, calcareous fen with Cladium mariscus, transition mire and quaking bog. The fluctuations of seasonal water level were defined in the range from dry substrate to short time flooding (up to two weeks). Leaf length and width of 30 flowering specimens of L. loeselii as well as angle between the leaf blade and the axis of the inflorescence were measured at each locality. Seed viability and root anatomy were examined in the laboratory. The leaf angle was significantly affected (P < 0.05) by water level in the studied habitat. The leaf length to width ratio in each population of L. loeselii did not show significant differences between the years, but was characteristic for the habitat type. L. loeselii sensitivity to the long-term flooding in the habitat is associated with a lack of aerenchyma in its roots. During short-term flooding specimens of L. loeselii can survive due to a shallow root system and hyponastic leaf movements. As evidenced by seed viability, the most favourable growth conditions for L. loeselii are in the habitats with partially water saturated substrate with balanced water supply.
EN
Seeds of Pyrus calleryana were sampled from 11 stations located in Poznań green areas. Three of them represented cultivar ‘Capital’, whereas the remaining eight represented cultivar ‘Chanticleer’. The viability of seeds was qualified by means of tetrazolium test. The results of staining test showed that viability of Callery pear seeds was high and ranged from 90 to 100%.
EN
The objective of this research was to determine the suitability of boiling water test in the prediction of seed viability in leek seed and to compare this test with standard germination and vigour test. Basic principle of boiling water test is the evaluation of radicle protrusion from the seeds immersed to boiling water for certain time. The viability of aged and unaged leek seeds in boiling water test was correlated with standard germination and vigour test. In this research, the mean germination of unaged leek seeds varied from 29,63% to 70,18% depending on the cultivars. While the range of boiling water test in unaged leek seeds was between 35,57% to 51,06%, the range for emergence was between 27,63% to 72,05%. The viability of aged and unaged leek seeds in boiling water test was correlated with standard germination test. According to the results of this research, it might be assessed that boiling water test is a rapid and simple method to measure seed viability by evaluating radicle protrusion of leek seeds in boiling water. In conclusion, boiling water test as a new viability test for leek seed could be used as a rapid method for determining the viability in leek seeds.
PL
Celem badań była ocena przydatności testu z użyciem wrzącej wody do określenia żywotności nasion pora i porównanie uzyskanych przy jego pomocy wyników w rezultatami testów standardowych zdolności kiełkowania i wigoru. Podstawową zasadą tego testu jest ocena stopnia przebicia okrywy nasiennej przez korzonek zarodkowy u nasion umieszczonych przez określony czas we wrzącej wodzie. Żywotność nasion pora poddanych i nie poddanych sztucznemu postarzaniu była zgodna z wynikami powszechnie stosowanych testów kiełkowania i wigoru. Średni procent nasion pora nie poddanych starzeniu wahał się od 29,63 do 70,18 zależnie od odmiany, podczas gdy wyniki uzyskane przy użyciu testu wrzącej wody wahały się w granicach 35,57 - 51,06%, a zakres wschodów roślin - w granicach 27,63 - 72,05%. Na podstawie otrzymanych wyników można uznać test wrzącej wody jako szybką i prostą metodę, przydatną do oceny żywotności nasion pora. Polega ona na ocenie przebicia okrywy nasiennej przez korzonek zarodkowy u nasion, które umieszczono we wrzącej wodzie. Reasumując, test we wrzącej wodzie jest nową metodą oceny żywotności nasion pora i może być wykorzystany do szybkiej oceny tej cechy.
EN
Xanthium sibiricum, an annual weed, unexpectedly and dramatically occupied the exposed drawdown area after water had been impounded for the first time in the newly created Three Gorges Reservoir in China. In order to explain this phenomenon and establish an appropriate management strategy, the effects of constant submersion on seed viability and germination of X. sibiricum were investigated at two constant temperature regimes (25oC and 30oC) under artificial laboratory conditions. The results indicated that the seeds of X. sibiricum exhibited a high level of tolerance of submersion and up to 99% of seeds were viable in each treatment regime. The effect of submersion on germination was not obvious at 25oC until the submersion was prolonged for 180 days, while at 30oC the eventual germination rate of X. sibiricum, even after submergence for only one day, was significantly improved. The speed of germination was also consistently accelerated by prolonged periods of submersion. The proportion of seeds that germinated in all treatments combined was less that 56% due to seed dimorphism, thereby providing a seed bank. We conclude that the interaction between long-term winter flooding and high temperature in summer is the major reason that X. sibiricum was able to occupy the newly exposed drawdown area in the absence of competition. These findings provided further insight into how germination strategy and reservoir water-management regime contributed to this dramatic species outbreak.
EN
Fruits of large-leaved lime dried to 10% may be stored for 16 years in sealed containers at –3°C without loosing seed viability. Dormancy of seeds, extracted from hard fruit coats, may be released after chemical scarification in concentrated sulphuric acid for 10 minutes, followed-by stratification without any medium (chilling) at the temperature of 3°C, for 20–24 weeks, i.e. until the first seeds start to germinate. After such pretreatment, during the germination test conducted at alternating temperatures 3~15°C (16 + 8 hours/day) seeds germinate near 90% in several weeks. For seedling production scarified and stratified seeds should be sown in early spring into trays under a plastic tunnel which ensures a high percentage of seedlings emergence. Sowing of the pretreated seeds in spring in a open nursery gives poor results.
EN
Artificial hybridization experiments revealed a relatively high degree of crossability between Pinus sylvestris and P. mugo, ranging between 0.15 and 0.98. Differences between P. sylvestris x P. mugo and reciprocal hybrids of the species were observed. Hybrid seeds of P. sylvestris x P. mugo shared higher germination potential than seeds from selling, controlled intraspecific crossing, and open pollination. The corresponding value in reciprocal combination was slightly lower. The hybrid nature of the seeds obtained was shown by restriction analysis of chloroplast DNA (cp DNA) using the trnV-trnH/Hinf I primer-enzyme combination.
EN
The study addressed different biological aspects of Juniperus oxycedrus subsp. macrocarpa, including female cone production, biometric analysis of mature cones and seeds, and their viability. The results indicate that the proportion of aborted female cones was very high. In the four populations studied, cone diameter and weight and seed length and weight were closely related. The distribution of numbers of seeds with and without embryos in mature cones was examined, as well as the frequency of numbers of seeds with embryos. The general trend in all populations was three seeds per mature cone, of which only one or two showed embryos. Tetrazolium tests showed the percentage of viable seeds to be lower.
EN
Liparis loeselii (L.) Rich. is an endangered orchid species which occurs in wetland habitats. For successful conservation of this orchid it is necessary to recognize its adaptive traits and special habitat requirements. Therefore, we examined morphological and anatomical traits in relation to the water level, cover of vascular plants and seed viability of L. loeselii in habitats with different water level fluctuations. Field studies of L. loeselii were carried out in nine localities and four habitat types: alkaline fen, calcareous fen with Cladium mariscus, transition mire and quaking bog. The fluctuations of seasonal water level were defined in the range from dry substrate to short time flooding (up to two weeks). Leaf length and width of 30 flowering specimens of L. loeselii as well as angle between the leaf blade and the axis of the inflorescence were measured at each locality. Seed viability and root anatomy were examined in the laboratory. The leaf angle was significantly affected (P < 0.05) by water level in the studied habitat. The leaf length to width ratio in each population of L. loeselii did not show significant differences between the years, but was characteristic for the habitat type. L. loeselii sensitivity to the long-term flooding in the habitat is associated with a lack of aerenchyma in its roots. During short-term flooding specimens of L. loeselii can survive due to a shallow root system and hyponastic leaf movements. As evidenced by seed viability, the most favourable growth conditions for L. loeselii are in the habitats with partially water saturated substrate with balanced water supply.
EN
It was proposed previously that passive dispersal by migratory aquatic birds explain the widespread distribution of many wetland organisms. Several experimental studies have shown that many widespread wetland plant species can be readily dispersed within the guts of Anatidae. However, it is unclear whether plants with a more restricted distribution are able to disperse via waterbirds. This paper addresses the dispersal ability and germination ecology of the little-known Hungarian milkvetch Astragalus contortuplicatus, which occurs on banks of continental rivers and has a limited and unpredictable distribution. To test whether limited capacity for endozoochory by waterfowl could explain the sporadic appearance of this species, we force-fed ten captive mallards (Anas platyrhynchos) with 100 milkvetch seeds each. Droppings were collected for up to 45 h after feeding. Intact and viable seeds were found in the droppings of each mallard, and altogether 24.7% of seeds fed were recovered intact. The proportion of retrieved seeds that germinated (27.0%) was significantly higher than that of untreated control seeds (0.5%), but significantly lower than that of mechanically scarified seeds (96.0%). Retrieved seeds that germinated developed into healthy mature plants. Given the average flight velocity of mallards, seeds of A. contortuplicatus may travel up to 1600 km inside the digestive tract of migrating individuals. Our results suggest that avian vectors may be more important for the dispersal of rare higher plants (especially those with a hard seed-coat) than hitherto considered. Moreover, they suggest that rarity does not necessarily indicate limited dispersal ability, and may instead be explained by specific habitat requirements.
PL
Obiektem badań były mieszańce międzygrupowe lilii Mieszańce Orientalne, Mieszańce Longiflorum, Mieszańce Trąbkowe i Mieszańce Azjatyckie. Określono skuteczność krzyżowania różnych gatunków lilii przy zastosowaniu dwóch różnych sposobów zapyleń oraz wspomagania kiełkowania otrzymanych zarodków w kulturach in vitro. Do udanych krzyżowań zaliczono ‘Montreux’ x ‘Rosato’, ‘Montreux’ x ‘Miami’, ‘Berlin’ x ‘Connecticut King’. L. regale x ‘Apeldoorn’ i ‘Miss Alice’ x ‘La Reve’. Pięć pierwszych mieszańców zawiązało po jednym nasionku. Z krzyżowań między L. regale x ‘Apeldoorn’ i ‘Miss Alice’ x ‘La Reve’ L. regale x ‘Apeldoorn’ i ‘Miss Alice’ x ‘La Reve’ otrzymano po 3 nasiona, między L. regale x ‘Miss Alice’- 2 nasiona, a ze skrzyżowania odmian L. regale x ‘Apeldoorn’ i ‘Miss Alice’ x ‘La Reve’- 5 nasion. W sumie z 452 krzyżowań udało się otrzymać 16 nasion z wykształconymi zarodkami. Wszystkie odznaczały się słabą żywotnością. W kulturach in vitro zainicjowano do wzrostu tylko jedno z zawiązanych nasion ‘Berlin’ x ‘Connecticut King’.
EN
The studies were conducted on the inter-group lily crosses - Oriental hybrids (10), Longiflorum hybrids (3), Trumpet hybrids (2) and Asiatic hybrids (9). The work aimed at determining the efficiency of crossing various lily species using two different methods of pollination and assisting the germination of the embryo in the in vitro cultures. Among the successful crossings were: ’Montreux’ x ’Rosato’, ’Montreux’ x ‘Miami’, ‘Berlin’ x ‘Connecticut King’, L. regale x ‘Apeldoorn’ and ‘Miss Alice’ x ‘La Reve’. The first five crossbreds produced one seed each. Crossing between L. regale and ‘Apeldoorn’, ‘Miss Alice’ and ‘La Reve’ resulted in three seeds each, L. regale and ‘Miss Alice’ - 2 seeds, L. regale and ‘Apeldoorn’, ‘Miss Alice’ x ‘La Reve’ - 5 seeds each. In total out of 452 crossings 16 seeds were obtained with a well formed embryo. All were characterized with a poor vitality. In the in vitro cultures only one seed was initiated for growth - ‘Berlin’ x ‘Connecticut King’.
PL
W badaniach nad żywotnością i wigorem nasion gorczycy białej aplikowano donasiennie wyciągi wodne z kompostów na bazie komunalnego osadu ściekowego z 40% udziałem słomy żytniej i liści z drzew. Stwierdzono nieduże różnice pomiędzy działaniem wyciągów z kompostów - zarówno jednorocznych, jak i dwuletnich. Krótsze, tj. sześciogodzinne, moczenie nasion w wyciągach okazało się efektywniejsze w odniesieniu do wszystkich badanych wskaźników żywotności i wigoru nasion niż dwudziestoczterogodzinne, bez względu na rodzaj i stężenie wyciągów kompostowych. Wzrastające stężenie omawianych wyciągów powodowało nieznaczne ograniczenie energii i zdolności kiełkowania nasion, ale równocześnie w niewielkim stopniu zwiększało wartości wskaźników wigoru, tj. długość siewek oraz ich świeżą i suchą masę.
EN
The water extracts from composts made from municipal sewage sludges with 40% share of rye straw and tree leaves were used for the evaluation of vitality and vigour of white mustard seeds. No significant differences were observed between the effects of extracts made from one-year and two-year old composts. Independently from the type and the concentration of compost extracts, the soaking of seeds in the extracts for six hours was more effective for all tested indexes of vitality and vigour of seeds as compared with a longer period (24 h). The increase of the concentration of the extracts resulted in a slight limitation of energy and ability for the emergence of seeds, but simultaneously, the tendency for the increase of the seedlings length and their fresh and dry matter was observed.
PL
Uzyskanie kwalifikowanego materiału siewnego wymaga wykonania szeregu czynności fizycznych, mających źródło w znajomości praw rządzących procesami rozdzielczymi. Podstawą ich są różnice w niektórych właściwościach fizyko-mechanicznych pomiędzy poszczególnymi składnikami mieszanin ziarnistych. Znajomości takiej wymaga ponadto konstruowanie podzespołów maszyn i urządzeń do uszlachetniania materiału siewnego oraz efektywna eksploatacja czyszczalni i sortowników mechanicznych oraz innych maszyn do obróbki nasion. Zarówno współczesne jak i dawne konstrukcje separatorów są oparte na doświadczeniu praktyków, a nie teoretycznych koncepcjach mających źródło w gruntownej znajomości agrofizyki nasion. Ze względu na powyższe niezbędna jest szczegółowa analiza cech rozdzielczych dębu, uwzględniająca także cechy nie wykorzystywane w leśnictwie do tej pory, a które powinny być wzięte pod uwagę w konstrukcji przyszłościowych rozdzielaczy uniwersalnych. Mechaniczna separacja nasion wymaga znajomości ich podstawowych parametrów geometrycznych, w tym wielkości i kształtu. Pomiary wspomnianych parametrów wykonano korzystając z komputerowej analizy obrazów nasion, uzyskanych z aparatu cyfrowego. Celem pracy była analiza wielkości i kształtu żołędzi dębu szypułkowego (Quercus robur L.) opisana współczynnikami kształtu, z uwzględnieniem przynależności do klas żywotności. Przyjęto klasyfikację nasion na 3 frakcje pomiarowe, biorąc za podstawę rozwój zarodka oraz szacowaną na tej podstawie spodziewaną zdolność kiełkowania nasion. Wyniki badań wykazały, że cechy geometryczne żołędzi nie mogą być uznane za cechy rozdzielcze, zatem nie ma możliwości przeprowadzenia procesu separacji w oparciu o wielkość nasion. Podczas badań zaobserwowano także, że tradycyjny sposób suszenia żołędzi, celem przygotowania do przechowywania, nie umożliwia uzyskania jednorodnej wilgotności końcowej. Jedynie nasiona żywotne o dużych zarodkach wykazują właściwą wilgotność, znacząco wyższą od pozostałych. Stanowi to zapewne jedną z przyczyn spadku zdolności kiełkowania nasion w trakcie przechowywania.
EN
Obtaining certified seed material requires a number of physical actions which in turn call for the knowledge of rules governing separation processes. They are based on the recognition of some physical and mechanical differences in properties between various components of seed mixtures. This kind of knowledge is further required when designing and building various subassemblies of machinery and equipment used in improving seed material, in operating effectively seed cleaners and mechanical seed sorters as well as other machinery used in seed processing. Both contemporary and older designs of separators utilise the experience of practitioners rather than the theoretical concepts rooted in deep knowledge of agrophysics of seeds. In view of the above, there is a necessity of a detailed analysis of separation-related properties of the Pedunculate Oak acorns, including the features not yet used in forestry, but which should be considered when designing future universal separators. Mechanical separation of seeds requires knowledge about their fundamental planimetric parameters like linear dimensions, crosssection areas and cross-section circumferences. Measurements of the mentioned parameters are quite simple with the help of computer image analysis. Seed images can be obtained, among others, from the digital camera. The aim of this work was an analysis of size and shape of the Pedunculate Oak acorns (Quercus robur L.) determined by shape coefficients, in correlation with their viability. A classification of seeds was adopted dividing them into three measurement fractions, based on the development of the embryo and the predicted seed germination capacity. The results of the studies showed that geometric properties of the acorns cannot be regarded as separation properties, so the separation of the seeds cannot be performed by basing the process on the size of seeds. In the course of the study it was also noted that the tradi-tional method of drying acorns in order to prepare them for storage failed to assure uniform final moisture content. The most valuable seeds, vital ones with large seed embryos, have significantly higher moisture content than other seeds. This is perhaps the reason behind a drop in the germina-tion capacity of seeds during storage.
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