Wyniki wyszukiwania - Biblioteka Nauki

1

Roznicowanie niedokrwistosci

100%

Sitarska E. , Klucinski W. , Cetnarowicz A.

Magazyn Weterynaryjny

|

2003

|

tom 12

|

nr 09

35-36

2

Zastosowanie analizy makrorestrykcyjnej do roznicowania wybranych szczepow z grupy Bacillus cereus

88%

Niemcewicz M. , Bartoszcze M.

|

tom 62

|

nr 09

1065-1070

EN

The aim of the study was to apply macro-restriction analysis in order to differentiate selected strains of the Bacillus cereus group. Three different restriction enzymes (SfiI, NotI and SmaI) were used during the research process and it was confirmed that SmaI is the best restriction enzyme applied in macrorestriction analysis of the Bacillus cereus group. The study used 89 Bacillus cereus group strains, and an identical restriction pattern generated by restriction enzymes was confirmed among 23 of the studied Bacillus anthracis strains. Two groups of strains (four and two strains) among 19 Bacillus sp. Ba 813 and three groups (three, two and two strains) among 44 strains of Bacillus thuringiensis showed identical restriction profiles. The similarity of studied strains was ascertained on the basis of the Dice coefficient, and confirmed that strains showing the same restriction profiles were identical (100% similarity). The similarity between the remaining strains of the Bacillus cereus group and the Bacillus anthracis strains oscillated between 20% to 78.6%, while the similarity of these strains to each other oscillated from 15.4 % to 100%. Dendrograms were also constructed using the UPGMA method.

3

Niekonwencjonalne metody różnicowania grzybów z rodzaju Fusarium: zastosowanie testu wegetatywnej zgodności

88%

Rataj-Guranowska M. , Walkowiak I.

Postępy Mikrobiologii

|

1991

|

tom 30

|

nr 3

4

RAPD jako metoda roznicowania i identyfikacji drozdzy

88%

Robak M. , Baranowska K. , Barszczewski W. , Wojtatowicz M.

Biotechnologia

|

2005

|

nr 4

142-155

5

Roznicowanie komorek nablonkowych grasicy

75%

Brelinska R.

|

tom 15

151-176

6

Licytacja wokół "Różnicowania"

75%

Pokora-Malinowska M.

|

2012

|

nr 09

7

Wewnatrzgatunkowe roznicowanie szczepow Malassezia pachydermatis z wykorzystaniem PCR-REA

75%

Nowakiewicz A. , Ziolkowska G.

Medycyna Weterynaryjna

|

2008

|

tom 64

|

nr 06

822-827

EN

The aim of the study was to make an attempt at showing the intraspecies heterogenecity of Malassezia pachydermatis strains with regards to their origin (strains isolated from healthy dogs and with otitis externa symptoms). The study included 41 strains of Malassezia pachydermatis species isolated in a pure culture from dogs with clinical otitis externa symptoms (n = 20), clinically healthy dogs (n = 20) and a reference strain, M. pachydermatis (CBS7925). In order to isolate the genetic material from the fungal cells, the following four procedures were selected: mechanical, enzymatic, thermal and chemical. Considering the yield and repeatability of a method for the genomic DNA extraction, a mechanical method was applied. The genetic material research of each strain was performed according to PCR-REA technique with the amplification of three genome regions: ITS, LSU rRNA and a gene encoding beta-tubuline. The ITS and LSU rRNA regions were amplified employing the standard PCR reagents, whereas the region coding beta-tubuline with the so called touch down. The obtained amplification products were subjected to restrictive analysis by means of the following enzymes: EcoRI, Ncol, Hinfl, Alul, and Eco881 (Aval). The performed investigations made it possible to reveal the genotypic differentiation within M.pachydermatis species as well as some correlation between a genotypic profile and the origin of a strain (from healthy animals or with otitis externa symptoms), which may imply the existence of genetic conditioning of the Malassezia strains’ pathogenicity.

8

Komórki macierzyste - rozpoznawanie oraz znaczenie w homeostazie płuc

75%

Biczysko M. , Biczysko W.

Postępy Biologii Komórki

|

2015

|

tom 42

|

nr 3

9

Zmiennosc szczepowa u przemyslowych drozdzy piwowarskich i winiarskich Saccharomyces cerevisiae przy zastosowaniu metody RAPD

75%

Misiewicz A. , Dzielakowska A. , Goryluk A. , Wroblewska-Kabba S.

Prace Instytutów i Laboratoriów Badawczych Przemysłu Spożywczego

|

2009

|

tom 64

43-56

10

Nowoczesne metody identyfikacji i roznicowania starterow mleczarskich

75%

Lewicka K.

Przegląd Mleczarski

|

1997

|

nr 09

300-302

11

Zastosowanie analizy makrorestrykcyjnej i reakcji PCR w odroznianiu bezplazmidowych szczepow Bacillus anthracis od szczepow przejsciowych [Bacillus sp. Ba 813]

63%

Niemcewicz M. , Bartoszcze M.

|

tom 62

|

nr 06

658-662

EN

The aim of the study was to use selected genetic analysis to differentiate plasmid cured strains of Bacillus anthracis from transitional strains (Bacillus sp. Ba 813). Two different research techniques (macro restriction analysis and PCR) were used and they confirmed that the PCR technique facilitated detecting the presence of the Ba 813 chromosomal sequence in both strains (plasmid cured Bacillus anthracis strain and Bacillus sp. Ba 813). This result indicated that applying the PCR method to differentiate plasmid cured Bacillus anthracis strains from Bacillus sp. Ba 813 was not possible. The macro restriction method, however, facilitated a precise differentiation of plasmid cured Bacillus anthracis strains from transitional Bacillus sp. Ba 813.

12

Biegunki u psów - podwójna rola pokarmu

63%

Glowacka M. , Ceregrzyn M.

|

2016

|

tom 25

|

nr 09

13

Oogeneza i roznicowanie sie jajnika u hybrydogenetycznego mieszanca, Rana esculenta L.

63%

Ogielska M.

|

1995

|

tom 32

42

14

Roznicowanie paciorkowcow grupy B pochodzacych od ludzi i zwierzat

63%

Dabrowska-Szponar M. , Galinski J. , Kasprzynski M.

Medycyna Weterynaryjna

|

2002

|

tom 58

|

nr 06

436-538

15

Rodzaje komórek w badaniach cytologicznych: czy to nowotwór?

63%

Allison R. W.

|

nr 11-12

16

Nowe dane o klasyfikacji i roznicowaniu bakterii z rodzaju Aeromonas i ich praktyczne znaczenie w ichtiopatologii

63%

Kozinska A.

Postępy Mikrobiologii

|

2001

|

tom 40

|

nr 1

43-62

17

Roznicowanie szczepow Campylobacter jejuni metoda analizy polimorfizmu przypadkowo amplifikowanego DNA

63%

Wieczorek K. , Osek J.

|

tom 62

|

nr 06

663-664

EN

Campylobacter, especially C. jejuni, is now recognized as a major cause of bacterial enteritis in people in most developed countries. The diversity within C. jejuni species may be described by both phenotypic and genotypic methods. In recent years, molecular methods, such as the restriction of fragment length polymorphism (RFLP), fla typing or random amplified polymorphic DNA (RAPD), have been proposed as alternative tests to phenotypic methods for typing of Campylobacter. This study was carried out to investigate the genetic variation among isolates C. jejuni by RAPD-PCR. The analysis of 19 isolates of C. jejuni performed with two primers (1254 and OPA-11) generated multiple amplification products, with sizes between 300 and 2000 bp. The UPGMA analysis of the fingerprints classified these strains to 10 clonal groups. The discriminatory index D=0.73 indicates a high differentiation ability of the method. Such data are valuable in epidemiological studies and for investigating the distribution of genotypes in different environments.

18

Zastosowanie metody multiplex PCR-RFLP do identyfikacji i roznicowania szczepow enterokokow

63%

Jarzembowski T.

Medycyna Doświadczalna i Mikrobiologia

|

2004

|

tom 56

|

nr 2

133-137

PL

Celem badań było ustalenie czy modyfikacja metody MLST opracowanej przez Sreedhar i wsp. (2002) do różnicowania szczepów enterokoków pozwala na uzyskanie wyników wystarczających do realizacji powyższego zadania. Wprowadzona modyfikacja polegała na zastąpieniu sekwencjonowania produktu amplifikacji analizą restrykcyjną. Wyniki analizy 32 szczepów Enterococcus faecalis izolowanych w regionie gdańskim potwierdziły przydatność powyższej modyfikacji do różnicowania szczepów enterokoków.

EN

The present study proposed use of modified multilocus sequence typing (MLST) approach, for subspecies differentiation of Enterococcus faecalis isolates. We disgesed with Sspl endonuclease product of amplification of three E. faecalis antigen-encoding genes (ace, encoding a collagen and laminin adhesin; efaA, encoding an endocarditis antigen; and salA, encoding a cell wall associated antigen) and one housekeeping gene (pyrC) of 32 E. faecalis isolates MLRA analysis of polymorphic regions of these four genes identified 23 distinct types 21,4 to 100% identity among the 32 isolates. Further studies are needed to evaluate the value of proposed method.

19

Roznicowanie BSE i trzesawki w poglowiu owiec i koz

63%

Polak M. P. , Larska M. , Rozek W. , Zmudzinski J. F.

|

tom 62

|

nr 11

1298-1301

EN

The use of BSE-contaminated meat and bone meal for feeding sheep and goats may be the cause of transmitting the BSE agent into small ruminant populations. Experimental studies have shown that sheep which are fed cattle brain homogenates from BSE cases can succumb to a BSE-like disease. The distribution of BSE agents in these sheep is similar to scrapie and a wider range of organs is affected when compared with BSE in cattle. Thus, the possibility of crossing the species barrier between sheep and man cannot be excluded (as has been shown with BSE and its variant - Creutzfeldt-Jakob disease in man). The paper describes implementing one of the approved immuno-blot methods used for discriminating between BSE and scrapie in a small ruminant population. The use of two monoclonal antibodies of which only one reacts with scrapie and atypical scrapie samples facilitates differentiation between BSE and scrapie positive samples from sheep and goats.

20

Zastosowanie techniki PCR i analizy polimorfizmu dlugosci fragmentow restrykcyjnych DNA [PCR/RFLP] do roznicowania szczepow herpeswirusa bydlecego typu 1 [BHV-1]

63%

Salwa A. , Burkiewicz A. , Anusz K.

Medycyna Weterynaryjna

|

2000

|

tom 56

|

nr 11

725-729