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1

Germination and In Vitro Propagation of Gundelia tournefontii as an Important Medicinal Plant

100%

Shatnawi M. , Majdalawi M. , Shahrour W. , Abu-Zahra T. R. , Al-Tawaha A. R.

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tom Vol. 23, iss. 1

57--64

EN

Medicinal plants are important sources of medical and many other pharmaceutical goods. The conventional propagation scheme is the main means of proliferation and takes a long period of time because of poor germination and also low clonal uniformity. Rapid shoot multiplication of Gundelia tournefontii was attained from meristems on Murashige and Skoog (MS) basal medium containing 6-Benzyladenine (BAP) or kinetin, with the adding of 30 g/L sucrose. The highest number of new microshoots per explant (9.2) was attained on the MS medium enhanced with BAP and 0.05 mg/L IBA. After 13 to 14 days, microhoots started rooting on the MS medium enhanced either Indole3-butyric acid (IBA), Indole-3-acetic acid (IAA), or Naphthalene acetic acid (NAA) with the addition of 30 g/L sucrose. Using 100 to 300 ppm Gibbreline GA3 resulted in an increase in germination percentage when soaking with GA3 for 12 hours. Gamma radiation had a significant effect on the growth of the in vitro explants. Gamma radiation had a significant effect on the germination percentage of G. tournefontii seeds. The in vitro propagation plant of G. tournefontii plants could be used for the marketable clonal propagation of this important species or for future studies.

2

Analysis of geometrical features of a developing lateral root by means of biophysical tools

86%

Szymanowska-Pulka J. , Lipowczan M. , Karczewski J.

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nr 3

3

Vertical production of ‘Konstancin’ rose cuttings in the growth chamber under led light

86%

Matysiak B.

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2020

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tom 28

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nr 2

4

Anatomical structure and adventitious root formation in Rhododendron ponticum L. cuttings

86%

Strzelecka K.

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tom 06

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nr 2

EN

Cuttings of R. ponticum were treated or not with ‘Ukorzeniacz AB’ containing IBA and NAA, and placed in rooting medium (temp. 21°C). Transverse sections were made of the bases of the cuttings removed from the soil after 2; 3; 4; 5 and 6 weeks. Meaningful anatomical changes in anatomical structure of R. ponticum cuttings connected with wound-healing process could be seen during rooting. In first two weeks periderm in cortex and callus from cambium and phloem emerged as a response to wounding of cuttings. After 3 weeks initiation of wound xylem started in callus. It developed very intensively and did not depend on the way of cutting treatment. Application of auxins did not or not considerably influenced the anatomical changes in stem structure of R. Ponticum during rooting – proliferation of new xylem was similar in control cuttings and in cuttings treated with rooting stimulator ‘Ukorzeniacz AB’, although it stimulated initiation of root primordia. Adventitious root formation occurred only in cuttings treated with ‘Ukorzeniacz AB’, in which numerous root primordia could be observed after 3 and more weeks of rooting. In control cuttings there was none of them even after 6 weeks of rooting.

PL

Sadzonki różanecznika pontyjskiego, kontrolne i traktowane preparatem Ukorzeniacz AB zawierającym IBA i NAA, umieszczano w podłożu o temperaturze 21°C. Do analizy anatomicznej pobierano 3 sadzonki, po 2, 3, 4, 5 i 6 tygodniach od czasu umieszczenia ich w podłożu. Podczas ukorzeniania sadzonek zachodziły w nich kompleksowe zmiany dotyczące budowy anatomicznej, związane z procesem gojenia się rany. W pierwszym tygodniu, w odpowiedzi na zranienie podstawy sadzonek w obrębie kory pierwotnej powstała ochronna peryderma, a z kambium i komórek miękiszowych łyka następowała silna proliferacja kalusa, w którym po 3 tygodniach komórki miękiszowe różnicowały się w naczynia o siatkowatej perforacji. Zastosowane auksyny bardzo nieznacznie lub wcale nie wpływały na zmiany w budowie anatomicznej pędu sadzonek w trakcie ukorzeniania – tworzenie nowego drewna zachodziło z jednakową intensywnością w sadzonkach kontrolnych i traktowanych Ukorzeniaczem AB, wpływał on natomiast na powstawanie primordiów. Inicjacja korzeni przybyszowych zachodziła tylko w sadzonkach traktowanych Ukorzeniaczem AB, w których obserwowano liczne zawiązki korzeni przybyszowych po 3 i więcej tygodniach ukorzeniania. W sadzonkach kontrolnych nie obserwowano tworzenia się primordiów nawet po 6 tygodniach ukorzeniania.

5

Brassica oleracea var. botrytis: evaluation of in vitro potential of two commercial cultivars

72%

Hnatuszko-Konka K. , Gerszberg A. , Kowalczyk T. , Kononowicz A. K.

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nr 3

6

Localization of oxido-reductases and hydrolases during adventitious root primordium initiation and emergence in hypocotyl cuttings of Phaseolus vulgaris L.

72%

Kakkar R. K. , Rai V. K.

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tom 16

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nr 4

7

Occurrence and identification of aster yellows related phytoplasma in Gladiolus in Poland

72%

Kaminska M. , Rudzinska-Langwald A. , Korbin M.

Acta Physiologiae Plantarum

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1999

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tom 21

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nr 4

8

Adventitious root formation of chokeberry (Aronia melanocarpa Elliot) influenced by the pH of medium

58%

Zatyko J. M. , Molnar I.

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nr 1

9

The effect of incision wounding of cuttings and of a rhizogenesis promoter on the rooting of dawn redwood [Metasequoia glyptostroboides Hu et Cheng]

58%

Kolasinski M.

Electronic Journal of Polish Agricultural Universities. Series: Horticulture

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2006

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tom 09

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nr 1

EN

On the basis of conducted experiments on the rooting of dawn redwood cuttings it was found that the application of Seradix B No 1 mixed with Kaptan Zawiesinowy 50 (1:1, v:v) increased the number of rooted cuttings by 14.5% in comparison to the control. Moreover, an advantageous effect of the growth stimulator was observed on the number of secondary roots on a cutting. However, the application of a growth promoter did not have a marked effect on their length. Incision wounding of cuttings at their base had an adverse effect on the rooting percentage and the length of secondary roots. It results from the conducted observations that at the position of the incision wounding roots are not formed. It can be seen from the performed cross-section that dawn redwood regenerates roots from the cambium (cambial ring) and not from the callus. For this reason the labour-intensive procedure of incision wounding of cuttings is not recommended in the propagation of dawn redwood. The effect of two factors, i.e. incision wounding of cuttings and the application of a growth regulator, on the rhizogenesis process in dawn redwood was investigated in this study.