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Structure and functions of 5S rRNA.

100%

Barciszewska M. Z. , Szymański M. , Erdmann V. A. , Barciszewski J.

Acta Biochimica Polonica

2001

tom 48

nr 1

191-198

The ribosome is a macromolecular assembly that is responsible for protein biosynthesis in all organisms. It is composed of two-subunit, ribonucleoprotein particles that translate the genetic material into an encoded polypeptides. The small subunit is the site of codon-anticodon interaction between the messenger RNA (mRNA) and transfer RNA (tRNA) substrates, and the large subunit catalyses peptide bond formation. The peptidyltransferase activity is fulfilled by 23S rRNA, which means that ribosome is a ribozyme. 5S rRNA is a conserved component of the large ribosomal subunit that is thought to enhance protein synthesis by stabilizing ribosome structure. This paper shortly summarises new results obtained on the structure and function of 5S rRNA.

Interaction of a Transcription Factor IIIA Peptide Containing Two Zinc Fingers with 5S rRNA

88%

Giel-Pietraszuk M. , Mucha P. , Rekowski P. , Kupryszewski G. , Barciszewska M. Z.

2002

tom Vol. 76 / nr 6

815-822

The interaction of lupin ribosomal 5S RNA with a chemically synthesized peptide containing 60 amino acid, derived from Xenopus laevis transcription factor IIIA, is analyzed. The results show that such short fragment retains the ability of binding to 5S rRNA molecule, as shown by electrophoretic gel shift and RNase footprint assay. The peptide protects from hydrolysis with specific nucleases helix II and V of 5S rRNA.

The nucleotide sequence of ribosomal 5S RNA from Rhizobium meliloti: comparison with 5S rRNA of Agrobacterium

75%

Nalaskowska M. , Barciszewska M. , Barciszewski J.

1989

tom 36

nr 3-4

Structure and functions of 5S rRNA

63%

Barciszewska M. Z. , Szymanski M. , Erdmann V. A. , Barciszewski J.

Acta Biochimica Polonica

2001

tom 48

nr 1

The ribosome is a macromolecular assembly that is responsible for protein biosynthesis in all organisms. It is composed of two-subunit, ribonucleoprotein particles that translate the genetic material into an encoded polypeptides. The small subunit is the site of codon-anticodon interaction between the messenger RNA (mRNA) and transfer RNA (tRNA) substrates, and the large subunit catalyses peptide bond formation. The peptidyl- transferase activity is fulfilled by 23S rRNA, which means that ribosome is a ribozyme. 5S rRNA is a conserved component of the large ribosomal subunit that is thought to enhance protein synthesis by stabilizing ribosome structure. This paper shortly summarises new results obtained on the structure and function of 5S rRNA.