Wyniki wyszukiwania - Biblioteka Nauki

1

Rooting of stem cuttings of Hedera helix 'Arborescens' as affected by zinc treatment

100%

Szydlo W. , Pacholczak A. , Lukaszewska A.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

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2007

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tom 28

2

In vitro plant regeneration of coffee Senna (Senna occidentalis) from hypocotyl-derived callus

100%

Isah T. , Mujib A.

Acta Biologica Cracoviensia. Series Botanica

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2013

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tom 55

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nr 2

3

Influence of selected carbohydrates on rhizogenesis of shoots saucer magnolia in vitro

100%

Kamenicka A.

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nr 4

EN

Nodal segements were taken from juvenile shoots of mature 100 year-old trees of saucer magnolia (Magnolia x soulangiana Soul.-Bod.) and cultured on Standardi and Catalano medium supplemented with 1.33 µmol·dm⁻³ BA, 0.54 µmol·dm⁻³ NAA, 58 µmol·dm⁻³ sucrose and 6.0 g·l⁻¹ agar-agar. After 8 weeks, separated shoots were transferred to rooting medium with half-strength macronutrients (basal medium) supplemented with 0.3% activated charcoal and one of carbohydrates: arabinose, cellulose, fructose, galactose, glucose, lactose, mannose, rhamnose, ribose, sorbose, sucrose or xylose at 20 g·dm⁻³ and 7.0 g·dm⁻³ agar-agar. After 13 weeks of culture, shoot number, fresh and dry weight of shoots and roots, total root length and number of roots/per shoot were recorded. Percentages of rooted shoots were calculated. Fructose, mannose and xylose were the most effective carbon source on shoot proliferation followed by sucrose. The rooting response was induced by cellulose and xylose. Arabinose, rhamnose and sorbose inhibited root formation. The number of adventitious roots produced per shoot was stimulated by cellulose and xylose. Total biomass (shoot plus roots) of the plantlets was the highest at fructose and cellulose.

4

Effect of ammonium zinc acetate on rooting of stem cuttings in Physocarpus opulifolius

84%

Pacholczak A. , Szydlo W.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

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2008

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tom 29

5

Plant regeneration via organogenesis and somatic embryogenesis in Verbascum sinuatum L.

84%

Karamian R. , Ghasemlou F.

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nr 1

6

The effect of biostimulators and indole-3-butyric acid on rooting of stem cuttings of two ground cover roses

84%

Pacholczak A. , Nowakowska K.

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nr 1

7

Rooting of wild blueberry (Vaccinium spp.) cuttings from the Peruvian Northeast

84%

Tejada-Alvarado J. J. , Melendez-Mori J. B. , Vilca-Valqui N. C. , Huaman-Huaman E. , Oliva M.

Acta Agrobotanica

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2021

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tom 74

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nr 1

8

Study on propagation of shrub and pillar roses by hardwood cuttings with auxins preparations

84%

Monder M. J.

Acta Scientiarum Polonorum. Hortorum Cultus

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2016

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tom 15

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nr 4

EN

All rose cultivars are propagated by asexual methods and the one of them is hardwood cuttings rooting. The aim of the study was to verify the possibility of effective propagation of shrub and pillar roses of different origin by hardwood cuttings. The shoots were collected in autumn. The cuttings were prepared and planted in spring. The rooting were conducted in open field and greenhouse. There were used commercial rooting powders containing IBA or NAA: Ukorzeniacz AB aqua, Ukorzeniacz B aqua, Rhizopon AA 020 XX, Chryzotek beige 004 XX, Ukorzeniacz – korzonek D DS. Of the ten cultivars chosen for the present experiment, only two took root effectively, with good quality rooted cuttings: ‘Excelsa’ (31.2% in open field, 56.5% in greenhouse) and ‘New Dawn’ (18.7% in open field, 14.3% in greenhouse). Using commercial rooting stimulants did not guarantee an increase in the number or in the parameters of growth of the rooted cuttings. The most effective preparation was Chryzotek beige 004 XX for ‘Poppius’ (38.0%) rooted in the open field, and for ‘Kew Rambler’ (23.5%) in the greenhouse.

PL

Wszystkie odmiany róż uprawnych rozmnażane są wegetatywnie i jednym ze sposobów jest ukorzenianie sadzonek zdrewniałych. Celem badań była weryfikacja możliwości efektywnego rozmnażania parkowych i pnących róż o różnym pochodzeniu przez sadzonki zdrewniałe. Sadzonki pobierano jesienią, przechowywano i sadzono wiosną. Ukorzenianie przeprowadzono na polu doświadczalnym i w szklarni. Użyto komercyjnych preparatów wspomagających ukorzenianie w postaci pudrowej zawierających IBA lub NAA: Ukorzeniacz AB aqua, Ukorzeniacz B aqua, Rhizopon AA 020 XX, Chryzotek beige 004 XX, Ukorzeniacz – korzonek D DS. Spośród 10 ukorzenianych odmian tylko dwie ukorzeniały się efektywnie, a ich sadzonki miały dobrą jakość: ‘Excelsa’ (31.2% w polu, 56.5% w szklarni) i ‘New Dawn’ (18.7% w polu, 14.3% w szklarni). Użycie preparatów komercyjnych wspomagających ukorzenianie nie gwarantowało zwiększenie liczby ukorzenionych sadzonek ani poprawy ich parametrów wzrostu. Najbardziej efektywnym okazał się Chryzotek beige 004 XX dla ‘Poppius’ (38.0%) ukorzenianej w polu i dla ‘Kew Rambler’ (23.5%) w szklarni.

9

Callus induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297

84%

Rao A. S.

International Letters of Natural Sciences

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2015

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tom 48

EN

An efficient protocol for induction of callus and regeneration of a sugar cane var 93v297 has been developed and reported here. Callus induction from immature young leaf explants derived from 2-3-month-old plants was achieved on Murashige and Skoog’s (MS) medium supplemented with different auxins viz, 2,4-D, NAA and IAA. Among different auxins, 2, 4-D at 3.5mg/l + 0.5mg/l BAP was found favourable in inducing callus. Addition of coconut milk and BAP further enhanced the growth of callus maximum being on MS medium supplemented with 0.5mg/l BAP (3602.33±0.88mg). Calli were further evaluated for regeneration. MS medium supplemented with 1.0 mg/l BAP was found suitable where 100% calli regenerated with maximum number of multiple shoots per callus mass (41.40±0.89). Highest number of root emergence (28.33±1.16) and maximum root length (3.40±0.67cm) was achieved on MS medium supplemented with 3mgl/l NAA. The in vitro grown plants were transferred to polycups containing a mixture of sterilized sand, soil and cocopeet (1:1:1) for hardening. The hardened plants were transferred to green-house conditions where they survived with 90% frequency.

10

Effect of light wavelength on in vitro organogenesis of a Cattleya hybrid

84%

Cybularz-Urban T. , Hanus-Fajerska E. , Swiderski A.

Acta Biologica Cracoviensia. Series Botanica

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2007

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tom 49

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nr 1

113-118

EN

The effect of light wavelength on multiplication, tissue growth and pigment content was studied in Cattleya intermedia x C. aurantiaca microcutting cultures. The initial explants were shoots regenerated from protocorm-like bodies. Modified MS medium containing 5.0 mg·l-1 BA, 0.2 mg·l-1 zeatin and 1.0 mg·l-1 NAA, solidified with Difco agar, was used for adventitious regeneration of shoots and aerial roots. The rate of organ initiation depended on the wavelength of the monochromatic light applied. Red and blue treatments were effective in triggering photomorphogenesis in the evaluated material. The propagation coefficient reached 11.7 under red light, 10.6 under blue, 8.3 under white and 6.2 in darkness. Total chlorophyll and carotenoid content were highest in cultures illuminated with white light, gradually decreasing from the blue to the red and the far red treatments. Blue light treatment improved the efficiency of micropropagation and benefitted initiation of rhizogenesis and aerial root elongation, and the resulting plants were true to type.

11

The influence of AlgaminoPlant and route on rhizogenesis of stem cuttings of Cotinus coggygria “Royal Purple” and “Young Lady”

84%

Petelewicz P. , Pacholczak A. , Szydlo W.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

12

The effect of plant growth regulators and sucrose on the micropropagation of common lilac (Syringa vulgaris L.)

84%

Ilczuk A. , Jagiello-Kubiec K.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

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2015

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tom 36

13

Competence and determination for rhizogenesis in in vitro culture of parsley [Petroselinum sativum Soo.]

84%

Konieczny R.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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1999

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tom 41

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nr 1

14

The effect of etiolation and shading of stock plants on rhizogenesis in stem cuttings of Cotinus coggygria

84%

Pacholczak A. , Szydlo W. , Lukaszewska A.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 4A

EN

In order to improve vegetative propagation of a difficult to root Cotinus coggygria the stock plants were subjected to: etiolation, shading and spraying with IBA, combined with the application of two commercially available rooting powders. The IBA treatment was more suitable for rooting of C. coggygria cuttings than the NAA application and it enhanced rhizot genesis regardless of the form of auxin application (foliar application to a stock plant or a rooting powder used directly on cuttings) and the amount of light provided to stock plants. Etiolation did not improve rhizogenesis in stem cuttings, however, reduction of light intensity by 50 % and 96 % of the ambient prior to harvest of cuttings affected rooting positively. Positive effects of shading can be ascribed to changes in shoot anatomy, i.e. a weaker sclerenchyma development. Synergistic effect of shading and Miar auxin application can result from the increase in leaf blade area and/or thinner lower epiderm. Em hanced rooting in cuttings from shoots grown out under reduced light intensity was accompanied by decrease in the contents of total soluble sugars, soluble proteins and free ABA and by increase in total chlorophyll, free amino acids, polyphenolic acids and free IAA contents.

15

Rhizogenesis in shoot cuttings of several ornamental shrubs

84%

Szydlo W. , Pacholczak A.

Folia Horticulturae

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2003

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tom 15

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nr 2

16

The effect of auxins: IAA, IBA and NAA on rooting of Hebe buchananii [Hook] and Hebe canterburiensis [J.B.Armstr.] 'Prostrata' in vitro

84%

Dabski M. , Parzymies M.

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tom 06

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nr 1

EN

The effect of auxins: IAA, IBA and NAA in concentrations of 1.0, 2.5 and 5.0 mg·dm⁻³ on rooting of Hebe buchananii and Hebe canterburiensis ‘Prostrata’ in vitro was examined. Shoots used in the experiment were excised from aseptically grown shoots on 1/2 MS media. Auxins used in the experiment were showed to have a positive effect on rooting of shoots of Hebe buchananii and Hebe canterburiensis ‘Prostrata’ in vitro. The biggest number of rooted microshoots and the longest roots of Hebe buchananii formed in presence of IBA. On the media supplemented with IAA in concentrations of 2.5 and 5.0 mg·dm⁻³, IBA in concentration of 5 mg·dm⁻³ and NAA in concentration of 1 mg·dm⁻³ 100% of rooted shoots of Hebe canterburiensis ‘Prostrata’ were obtained. The biggest number of roots occurred on media with 2.5 and 5.0 mg IAA·dm⁻³. NAA added to the media caused callusing of the shoots base.

PL

Badano wpływ auksyn: IAA, IBA i NAA w stężeniach 1; 2,5 i 5 mg⋅dm⁻³ na ukorzenianie Hebe buchananii i Hebe canterburiensis ‘Prostrata’ in vitro. Pędy wykorzystane do doświadczenia uzyskano z ustabilizowanych kultur prowadzonych na zestalonej pożywce ½ MS. Stwierdzono, że zastosowane auksyny mają korzystny wpływ na ukorzenianie pędów Hebe buchananii i Hebe canterburiensis ‘Prostrata’ in vitro. Najwięcej ukorzenionych mirkosadzonek i najwięcej długich korzeni Hebe buchananii uzyskano w obecności IBA. Na pożywce zawierające IAA w stężeniu 2,5 i 5 mg·dm⁻³, IBA w stężeniu 5 mg·dm⁻³ oraz NAA w stężeniu 1 mg·dm⁻³ stwierdzono 100% ukorzenionych sadzonek Hebe canterburiensis ‘Prostrata’. Największą liczbę korzeni uzyskano na pożywkach uzupełnionych 2,5 i 5 mg IAA·dm⁻³ . Dodanie do pożywki NAA powodowało kalusowanie podstawy pędu.

17

Direct ex vitro rooting technique of micropropagated strawberry shoots

84%

Borkowska B. , Szczygiel A. , Pierzga K.

Journal of Fruit and Ornamental Plant Research

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1999

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tom 07

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nr 1

18

Effect of rooting preparations on protein, chlorophyll and carotenoid content in leaves of Rosa gallica 'Duchesse d'Angouleme' cuttings

67%

Monder M. J. , Niedzielski M. , Wolinski K.

Dendrobiology

|

2014

|

tom 72

EN

Popularisation of historical rose cultivars is important for overall increase of biodiversity within currently available assortment, however their vegetative propagation is rather difficult. Possible scenario behind it is that commonly used one-bud leafy cuttings have limited storage capacity due to the small size of the stem. We hypothesised that the treatment of cuttings this rose with rooting preparations would positively affect on the condition of their leaves. We tested the effect of preparations based on seaweed extracts (Bio Rhizotonic, Root Juice, Bio Roots) as well as commonly used root stimulants (Ukorzeniacz Aaqua and Ukorzeniacz Baqua containing 0,4% IBA or 0,2% NAA respectively) on the viability potential of the Rosa ‘Duchesse d’Angoulême’ cuttings. We have monitored the senescence process in leaves by the total soluble protein, chlorophyll and carotenoid content measurements. In order to test the potential effect of the mother plant and timing of shoots acquirement cuttings for the experiment were taken from mother plants at four different maturity stages (flower buds closed; all flowers open; just after petal fall and 7–14 days after petal fall). As expected the amount of the measured components in mother plants varied in individual phases of shoot maturity. This feature influenced the positive effect of rooting stimulants on cuttings viability. The most striking effects were in rooted cuttings derived from shoots collected just after flower petal fall. Observed increase in soluble protein and chlorophyll content did not correlate however with the percentage of obtained rooted cuttings. Based on that we can speculate that the biological basis of observed positive effect of natural rooting preparations on the ‘Duchesse d’Angoulême’ rose is complex and does not rely on the delay or inhibition of senescence process.

19

Effect of biopreparations Amino Total and Biochikol 020 PC on rhizogenesis in stem cuttings of Physocarpus opulifolius 'Dart's Gold' and 'Diabolo'

67%

Jacygrad E. , Pacholczak A.

|

tom 31

20

Anatomiczne aspekty rozmnazania fuksji [Fuchsia hybrida] za pomoca sadzonek

67%

Strzelecka K.

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nr 2

635-643

PL

Obserwacji zmian anatomicznych dokonywano w sadzonkach zielnych trzech odmian fuksji ogrodowej: ‘Eden Rock’, ‘Southgate’ i ‘Dancing Flame’, kontrolnych i traktowanych Ukorzeniaczem B2. Do analizy anatomicznej pobierano po 3 sadzonki każdej odmiany: przez pierwszy tydzień co 24 godziny, natomiast w drugim tygodniu co 48 godzin. Preparaty anatomiczne sporządzano z podstaw sadzonek długości 0,5 cm, zatapianych w parafinie. Wszystkie badane odmiany fuksji odznaczały się dużą zdolnością do regeneracji korzeni przybyszowych. Zarówno sadzonki kontrolne, jak i traktowane auksyną ukorzeniały się łatwo, ale szybkość procesu zależała od odmiany. U odmian ‘Eden Rock’ i ‘Southgate’ pierwsze korzenie ukazywały się po 8-9 dniach, natomiast u ‘Dancing Flame’ po 11. Zawiązki korzeni przybyszowych powstawały w strefie kambium i łyka. Pierwsze podziały komórkowe prowadzące do powstania zawiązka obserwowano już po 24 godzinach ukorzeniania, a początki zorganizowanego ułożenia komórek widoczne były po 5 dobach u ‘Eden Rock’ i ‘Southgate’ i 8 u ‘Dancing Flame’. Egzogenna auksyna, zawarta w Ukorzeniaczu B2, pobudzała powstawanie primordiów u dwóch pierwszych odmian, głównie poprzez zwiększenie ich liczby, nieznacznie wpływając na przyspieszenie procesu. Liczba zawiązków wynosiła od 4 do 8 w porównaniu do 2-5 w sadzonkach kontrolnych. W sadzonkach ‘Dancing Flame’ traktowanych Ukorzeniaczem B2 zawiązków korzeni było mniej niż w sadzonkach kontrolnych.

EN

Anatomical changes were observed in cuttings of Fuchsia hybrida ‘Eden Rock’, ‘Southgate’ and ‘Dancing Flame’, control and treated with rooting stimulator ‘Ukorzeniacz B2’ (containing NAA 0.2% and Benomyl 2.5%). Transverse sections were made of 0.5 cm cutting bases sampled every 24 hours during the first week of rooting and every 48 hour during next period. All cuttings rooted well, but the length of the process depended on the cultivar. In ‘Eden Rock’ and ‘Southgate’ cuttings, roots emerged from the stem after 8-9 days of rooting, whereas in ‘Dancing Flame’ after 11 days. Root primordia formed in the cambium and xylem region. First divisions, which led to root primordia formation, were observed after 24 hours while first signs of root primordia organization could be seen after 5 days in ‘Eden Rock’ and ‘Southgate’ and after 8 days in ‘Dancing Flame’ cuttings. Auxin, present in ‘Ukorzeniacz B2’ stimulated rhizogenesis, mainly through increasing the number of primordia in ‘Eden Rock’ and ‘Southgate’. 4-8 adventitious roots were observed in cuttings treated with rooting stimulator, whereas only 2-5 were present in the control ones. In the case of ‘Dancing Flame’ there where less primordia in cuttings treated with auxin than in control ones.