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2D-TLC and RP-HPLC determination of phenolic acids in Passiflor and herb of Passiflora incarnata L.

100%

Smolarz H. D. , Bogucka-Kocka A. , Garbarczyk P. Z.

nr 3-4

The following phenolic acids: protocatechuic, p-hydroxybenzoic, o-hydroxybenzoic, cafFeic, syringic, p-coumaric and ferulic were detected in herb of Passiflora incarnata L. and in "Passiflor" by 2D TLC method. Derivatisation was performed with known reagents and two new ones containing solution of copper (II) and cobalt (II). The amount of seven phenolic acids were detected by RP HPLC method. The "Passiflor" specimen contains the same set and similar content of phenolic acids as herb of Passiflora incarnata L.

W zielu Passiflora incarnata L. i w preparacie „Passiflor" metodą 2D TLC zidentyfikowano następujące fenolokwasy: protokatechowy, p-hydroxybenzoesowy, o-hydroxybenzoesowy, kawowy, syryngowy, p-kumarowy, ferulowy i wanilinowy. Do derywatyzacji oprócz tradycyjnych stosowano dwa nowe odczynniki zawierające roztwory miedzi (II) i kobaltu (II). Metodą RP HPLC oznaczono zawartość 7 fenolokwasów. Stosowany w lecznictwie preparat „Passiflor" posiada taki sam zestaw i podobną ilość fenolokwasów jak ziele Passiflora incarnata L.

Column switching and liquid chromatographic technique for the rapid determination of fenoxycarb insecticide residues in apples

84%

Michel M. , Krause A. , Buszewski B.

tom 10

nr 4

Fenoxycarb residues are analyzed by column switching and reversed-phase high performance liquid chromatography (RP-HPLC). The active ingredient is extracted from apples on a silica gel column using a n-hexane - diethyl ether mixture. The eluate is evaporated, dry residue dissolved in acetonitrile-deionized water, and injected into the liquid chromatograph with a column switching system (C8 columns), and a UV-photodiode array detector (UV - PDA). The analyte is quantified by the external standard method. The average recoveries of the active ingredient from the spiked sample are 81.3 +/- 3.2% and 80.3 +/- 5.8%, the coefficients of variation are 3.9% and 7.2% for fortification levels 0.1 mg/kg and 0.05 mg/kg, respectively, and the limit of quantification at lambda = 228 nm is 0.05 mg/kg. Labor and organic solvent uses are greatly reduced in comparison to the existing methods. The overall procedure allows a sample throughput of up to 30 samples per day. The method was applied to the determination of fenoxycarb residue in apples from treated orchards.