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EN
Spumaviruses belong to the family of Retroviridae, genus Spumavirus. They are also commonly known as foamy viruses or syncytial viruses because of inducing the spectacular cytopathic effect in tissue culture, which produces vacuolated “foamy” forms and the appearance of typical syncytia. Foamy viruses are widespread and have been isolated from many mammals, such as primates, pets and livestock animals. The resultant infection is persistent and infected individuals develop a strong antibody response. Although these viruses cause a strong cytophatic effect in cell cultures, they have never been linked to particular symptoms or pathalogical developments. During the last few years the research on spumaviruses has increased, mainly due to their possible zoonotic potential and thus a great deal of activities connected with the development of diagnostic assays and pathogenicity studies were carried out. This paper reviews the present stage of knowledge on spumaviruses found in humans and animals.
PL
Zbadano 9 nowo zsyntetyzowanych N-benzoilofenyloserynianów seskwiterpenów o budowie analogicznej do taksolu w kierunku ich przeciwwirusowej aktywności. Badania przeprowadzono w układzie in vitro na modelu zakażeń hodowli komórkowej Vero wirusem HSV-1Mc oraz w układzie in vivo, badając wpływ związków na przebieg zakażenia myszy retrowirusem Mo- MSV.
EN
The study comprised newly synthesized sesquiterpenoid analogs of taxol. The synthesis of the compounds was performed at the Institute of Organic Chemistry, Polish Academy of Sciences. Cytotoxicity of the compound was assessed using formazan method. In in vitro studies the cell cultures were infected with HSV-1Mc. The tested compounds were added in different concentrations to the cell culture after viral infection. Titer of the virus was expressed in TCIDW50/ml at particular stages of the experiments. In in vivo experiments NMRI mice were infected intramuscularly with a Moloney murine sarcoma virus (Mo-MSV). Tested compounds were administered to the mice intravenously on the day of virus inoculation. In Mo-MSV-infected mice dynamics of tumor progression and regression was assessed, as well as a mean time interval of tumor disappearance. Among the compounds tested: isovellerol-13-N-benzoyl-(2'R,3'S)-3'-phenylisoserinate, 5-deoxy-lactarolid B 8-[N-benzoyl-(2'R,3'S)-3'-phenylisoserinate] and isolactarorufin 8-epi-[N-benzoyl-(2'R,3'S)-3'-phenylisoserinate] showed significant antiviral activity in in vitro experiments. In in vivo experiments only lactarorufin A 8-[N-benzoyl-(2'R,3'S)-3'-phenylisoserinate] significantly inhibited the development of tumors and shortened the time of their total regression in the course of Mo-MSV infection.
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