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Isolation and chromosomal distribution of a novel Ty1-copia-like sequence from Secale, which enables identification of wheat-Secale africanum introgression lines

100%

Jia J. , Yang Z. , Li G. , Liu C. , Lei M. , Zhang T. , Zhou J. , Ren Z.

Journal of Applied Genetics

2009

tom 50

nr 1

25-28

A repetitive sequence of 411 bp, named pSaO5₄₁₁, was identified in the Secale africanum genome (Rᵃ) by random amplified polymorphic DNA (RAPD) analysis of wheat and wheat-S. africanum amphiploids. GenBank BLAST search revealed that the sequence of pSaO5₄₁₁ was highly homologous to a part of a Ty1 -copia retrotransposon. Fluorescence in situ hybridization (FISH) analyses indicated that pSaO5₄₁₁ was significantly hybridized to S. africanum chromosomes of a wheat-S. africanum amphiploid, and it was dispersed along the Secale chromosome arms except the terminal regions. Basing on the sequence of pSaO5₄₁₁, a pair of sequence-characterized amplified region (SCAR) primers were designed, and the resultant SCAR marker was able to target both cultivated rye and the wild Secale species, which also enabled to identify effectively the S. africanum chromatin introduced into the wheat genome.

Sequence heterogeneity of the envelope-like domain in cultivated allotetraploid Gossypium species and their diploid progenitors

100%

Hafez E. E. , Abdel G. A. A. , Paterson A. H. , Zaki E. A.

Journal of Applied Genetics

2009

tom 50

nr 1

17-23

Retroviral envelope (env)-like sequences in 2 cultivated allotetraploid cottons and their diploid progenitors have been identified and characterized in this study. DNA sequence analysis reveals that these sequences are heterogeneous. The observed sequence diversity, however, seems to preserve coding information. This is evidenced by the detection of the transmembrane domain (TM), which is the most conserved feature of the divergent retroviral env genes. The high ratio of synonymous to nonsynonymous changes suggests that these sequences are evolving under purifying selection. Phylogenetic analysis shows that Gossypium sequences closely cluster with a lineage of plant endogenous retroviruses that have an env-like gene. These results provide evidence for the antiquity and the wide diversity of env-like sequences in the Gossypium genome.

The telomere-specific non-LTR retrotransposons SART 1 and TRAS1 are suppressed by PIWI subfamily proteins in the silkworm, Bombyx mori

100%

Tatsuke T. , Sakashita K. , Masaki Y. , Lee J. M. , Kawaguchi Y. , Kusakabe T.

Cellular and Molecular Biology Letters

2010

tom 15

nr 1

118-133

The telomere structures in Bombyx mori are thought to be maintained mainly by the transposition of the specialized telomeric retroelements SART and TRAS. The silkworm genome has telomeric TTAGG repeats and telomerase, but this telomerase displays little or no activity. Here, we report that the transcription of the telomeric retroelements SART1 and TRAS1 is suppressed by the silkworm Piwi subfamily proteins BmAgo3 and Siwi. The silkworm Piwi subfamily was found to be expressed predominantly in the gonads and early embryo, as in other model organisms, but in BmN4 cultured cells, these proteins formed granules that were separate from the nuage, which is a different behaviour pattern. The expression of TRAS1 was increased in BmN4 cells when BmAgo3 or Siwi were silenced by RNAi. Our results suggest that B. mori Piwi proteins are involved in regulating the transposition of telomeric retroelements, and that the functional piRNA pathway is conserved in BmN4 cultured cells.

Assessment of genetic relationships among cultivated and wild pistachios (Pistacia vera L.) using molecular markers

100%

Pourian M. A. , Bakhshi D. , Aalami A. , Hokmabadi H.

Journal of Horticultural Research

2019

tom 27

nr 1

Presence of env-like sequences in Quercus suber retrotransposons

80%

Carvalho M. , Ribeiro T. , Viegas W. , Morais-Cecilio L. , Rocheta M.

Journal of Applied Genetics

2010

tom 51

nr 4

The main difference between LTR retrotransposons and retroviruses is the presence of the envelope (env) gene in the latter, downstream of the pol gene. The env gene is involved in their infectious capacity. Here we report the presence of env-like sequences in the genome of Quercus suber (cork oak), one of the most economically important Portuguese species. These gene sequences were isolated through DNA amplification between RNaseH conserved motifs and 3' LTR, based on the structure of copia retrotransposons. Phylogenetic analysis revealed that almost all the clones isolated are clustered with Cyclops-2, a Ty3-gypsy element identified in Pisum sativum, except one clustered with gypsy and copia retroelements found in different species. This suggests the existence of a potential ancestral sequence of the env gene, prior to the separation of Ty3-gypsy and Tyl-copia retrotransposons. Additionally, the isolated env-like sequences showed 26-39% of homology with env-like sequences characterized in viruses. The origin of env-like sequences in retrotransposons from host plant taxa is discussed.