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1
Content available remote Restriction analysis of genetic variability of Polish isolates of Tomato black ring virus.
100%
Jończyk M. , Borodynko N. , Pospieszny H.
Acta Biochimica Polonica
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2004
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tom 51
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nr 3
673-681
EN
Several different isolates of Tomato black ring virus (TBRV) have been collected in Poland from cucumber, tomato, potato and black locust plants. Biological tests showed some differences in the range of infected plants and the type of symptoms, which was the basis for selection of seven the most biologically different TBRV isolates. According to the sequence of TBRV-MJ, several primer pairs were designed and almost the entire sequence of both genomic RNAs was amplified. The RT-PCR products derived from all tested TBRV isolates were digested by restriction enzymes. On the basis of the restriction patterns, the variable and the conserved regions of the TBRV genome were defined and the relationships between the Polish TBRV isolates established.
2
Content available Differentiation of European brown hare syndrome and rabbit haemorrhagic disease viruses by amplification and restriction analysis of capsid protein gene fragment
88%
Chrobocinska M.
Bulletin of the Veterinary Institute in Puławy
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2000
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tom 44
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nr 2
EN
The aim of the studies was differentiation of EBHS and RHD viruses by amplification of the fragment coding the first part of the capsid protein (VP60) and digestion of this fragment by restriction endonucleases. The fragments of 4 strains of EBHS (two Polish isolated in 1992 and 1998; two standard Italian and French) and 5 strains of RHD (Polish strains isolated in 1988, 1994, 1997 and 1998) viruses were amplified by RT-PCR with the use of primers selected for each virus. The specific fragments of 726 bp of EBHS and 734 bp of RHD viruses were demonstrated after amplification with the use of primers selected for each virus. Negative results were obtained when primers selected for the RHD virus were used to amplify the fragment of EBHS virus and on the contrary. The hybridisation by the Southern blot method revealed the cross reaction of DIG-Iabelled probes prepared for each virus. The endonucleases BamH I and Pst I were used in the restriction analysis of fragments. The 734 bp fragments of all strains of RHD virus digested by BamH I and Pst I enzymes demonstrated the same restriction profiles. The 726 bp fragments of strains of EBHS virus were not digested by Pst I, whereas in two strains (standard Italian and Polish isolated in 1988) after digestion with BamH I different restriction profiles in comparison with RHD virus were demonstrated.
3
Cassava chloroplast DNA: Isolation, comparisons and cloning
75%
Yeoh H-H. , Joseph S. S.
Acta Physiologiae Plantarum
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1995
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tom 17
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nr 4
4
Identyfikacja drożdży występujących w żywności. Analiza regionów rybosomalnych
63%
Kowal K.
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tom T. 71, nr 2
26--28
PL
W identyfikacji drożdży wykorzystuje się metody klasyczne i ich modyfikacje polegające na ocenie cech fenotypowych oraz metody nowoczesne oparte na analizie polimorfizmu materiału genetycznego. Metody klasyczne są powszechnie stosowane, jednak ze względu na szybką i precyzyjną identyfikację coraz częściej wykorzystywane są techniki biologii molekularnej. W artykule opisano techniki stosowane w identyfikacji gatunkowej drożdży oparte na analizie regionów rybosomalnych (sekwencjonowanie, analiza restrykcyjna).
EN
There are different methods of yeast identification – classical methods and its modifications and modern methods based on polymorphism analysis of genetic material. Identification of yeast using conventional methods is labour-intensive and time-consuming. Therefore there is a reason to use molecular biology techniques that allow faster and more precise identification. This article presents molecular biology techniques of yeast identification, including analysis of ribosomal regions (sequencing, restriction analysis).
5
Restriction analysis of mtDNA in whitefish [Coregonus lavaretus] from Swaderki hatchery
63%
Brzuzan P. , Jagodzinski G.
Natural Sciences
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1998
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tom 01
6
Restriction analysis of genetic variability of Polish isolates of tomato black ring virus
63%
Jonczyk M. , Borodynko N. , Pospieszny H.
Acta Biochimica Polonica
|
2004
|
tom 51
|
nr 3
EN
Several different isolates of Tomato black ring virus (TBRV) have been collected in Poland from cucumber, tomato, potato and black locust plants. Biological tests showed some differences in the range of infected plants and the type of symptoms, which was the basis for selection of seven the most biologically different TBRV iso­lates. According to the sequence of TBRV-MJ, several primer pairs were designed and almost the entire sequence of both genomic RNAs was amplified. The RT-PCR products derived from all tested TBRV isolates were digested by restriction en­zymes. On the basis of the restriction patterns, the variable and the conserved re­gions of the TBRV genome were defined and the relationships between the Polish TBRV isolates established.
7
Content available Restriction analysis of capsid protein gene fragment of two Polish strains of European brown hare syndrome virus
63%
Chrobocinska M.
Bulletin of the Veterinary Institute in Puławy
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2000
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tom 44
|
nr 2
EN
Polish strains of EBHS virus isolated in 1992 (NP11/92) and in 1988 (L/98) were compared with standard Italian and French strains by restriction analysis of fragment coding the first part of capsid protein (VP60). The 726 bp fragment of capsid protein gene of strains tested was obtained by RT-PCR. The digestion of 726 bp fragments by BamH I endonuclease, revealed the same restriction profile for strain L/98 as Italian standard strain. These fragments of strain NP11/92 and French standard strain were not digested by BamH I. Endonuclease Sma I did not digested the amplified fragment of strains tested. The 726 bp fragment of strain L/98 demonstrated different restriction profiles for BsuR I and Hpa II enzymes in comparison with strain NP11/92 and standard strains. These results revealed differences in restriction profiles of fragment coding the first part of VP60 of Polish strains in comparison with standard strains and also between two Polish strains isolated in 1992 and 1998. These differences suggest the possibility of genotype changes of Polish EBHS strains isolated in different years.
8
Characteristics of genes encoding structural protein of Polish strains of goose parvovirus
51%
Wozniakowski G. , Samorek-Salamonowicz E. , Kozdrun W.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
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nr 2
119-122
EN
The aim of this study was to come up with molecular characteristics of Polish strains of goose parvovirus (GPV) on the basis of genes encoding their structural proteins. Ten field GPV strains and two vaccine strains: B-38 and MFP were used. The PCR- RFLP method based on three distinct endonucleases: HinfI, MboI, and RsaI, which had restriction sites within the sequences of examined genes, was applied. It was found that the homology of VP1, VP2, and VP3 among the studied strains ranged from 50% to 100%. The major differences in restriction patterns were found after application of HinfI, whereas 100% homology was observed after Rsal digestion. Significant differences were observed in restriction profiles of vaccine GPV strains. The study revealed that the application of PCR-RFLP for the analysis of VP1, VP2, and VP3 genes allowed for their molecular characteristics and differentiation between the vaccine and field strains.
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