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1

Preparative separation of two alkaloids from devil pepper radix (Rauvolfia verticillata [Lour.] Baill.) by ph-zone-refining counter-current chromatography

100%

Duan W. J. , Liu Q. , Zhao R. X. , Mu Y. , Guo L. P. , Li D. P. , Wang X.

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tom Vol. 30, no. 2

81--84

EN

A method was developed for the preparative separation of two alkaloids from the crude extract of the radix of Rauvolfia verticillata (Lour.) Baill. in a single run. The two-phase solvent system composed of petroleum ether–ethyl acetate–methanol–water (5:5:2:8, v/v), where triethylamine (40 mmol/L) was added to the upper organic phase as the stationary phase and hydrochloric acid (10 mmol/L) was added to the lower aqueous phase as the mobile phase, was selected for this separation by pH-zone-refining counter-current chromatography (PZRCCC). For the preparative separation, the apparatus was rotated at a speed 850 rpm, while the mobile phase was pumped into the column at 2 mL/min. As a result, 112 mg of reserpine and 21 mg of yohimbine were obtained from 3 g of crude extract in a single run. The analysis of the isolated compounds was determined by high-performance liquid chromatography (HPLC) at 230 nm with purities of over 91.0%, and the chemical identification was carried out by the data of electrospray ionization–mass spectrometry (ESI–MS) and nuclear magnetic resonance (NMR) spectroscopy. The technique introduced in this paper is an efficient method for preparative separation of reserpine and yohimbine from devil pepper radix. It will be beneficial to utilize medicinal materials and also useful for the separation, purification, and pharmacological study of Chinese herbal ingredients.

2

High performance liquid chromatography based quantification of reserpine in Rauwolfia tetraphylla L. and enhanced production through precursor feeding

86%

Rohela G. K. , Bylla P. , Pendli S. , Korra R. , Gandu R. , Reuben C.

Acta Chromatographica

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2022

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tom Vol. 34, no. 2

120--129

EN

Rauwolfia tetraphylla L., is an important medicinal plant in Apocynaceae family and is recognized as an alternative source to Rauwolfia serpentina L., in terms of anti-hypertensive alkaloid production i.e. reserpine. In view of this, the present study is conducted to estimate the reserpine content in different parts (leaf, stem and root) of field grown plants (2 years old), tissue cultured plantlets (R1) (two months old) and cell suspensions cultures (two months old with and without precursor feeding) of R. tetraphylla by using high performance liquid chromatography (HPLC) technique. Overall maximum content of reserpine (in %) was estimated from the root samples. Roots of field grown plants has recorded high percent of reserpine (0.39%) followed by roots of tissue cultured plantlets (0.35%) and root callus based cell suspension cultures (0.38 %) which was fed with precursor amino acid (100 mg/L of tryptophan). In control type of root callus based cell suspension cultures, reserpine content was quantified as 0.14%; by precursor feeding (100 mg/L of tryptophan) it was enhanced to 0.38%. In conclusion, the reserpine content (0.35 and 0.38%) produced by the roots of tissue cultured plantlets (R1) and 100 mg/L tryptophan fed root callus based cell suspensions was comparable to that of the reserpine content (0.39%) of root parts of field grown plants. The present study demonstrates the reserpine production by in vitro cell suspension cultures throughout the year without sacrificing the medicinal plants.

3

Up-regulation of spermidine/spermine N1-acetyltransferase (SSAT) expression is a part of proliferative but not anabolic response of mouse kidney.

86%

Dudkowska M. , Stachurska A. , Grzelakowska-Sztabert B. , Manteuffel-Cymborowska M.

Acta Biochimica Polonica

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2002

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tom 49

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nr 4

969-977

EN

A differential expression pattern of spermidine/spermine N1-acetyltransferase (SSAT), the enzyme critical to proper homeostasis of cellular polyamines, is reported in mouse kidney undergoing hyperplasia and hypertrophy. We have shown that SSAT activity and SSAT mRNA are significantly induced by antifolate CB 3717 and folate that evoke a drug-injury-dependent hyperplasia. In contrast, SSAT activity is down-regulated in the testosterone-induced hypertrophic kidney, while SSAT mRNA is positively controlled by this androgen. Catecholamine depletion evoked by reserpine drastically decreases the folate-induced activity of S-adenosylmethionine decarboxylase (AdoMetDC), which limits polyamine biosynthesis, but has no effect on SSAT activity augmented by CB 3717. Our results document that the increased SSAT expression solely accompanies the proliferative response of mouse kidney, and suggest the importance of post-transcriptional regulation to the control of SSAT activity in both hyperplastic and hypertrophic experimental models.

4

Antidepressant-like effect of 1,2,3,4-tetrahydroisoquinoline in reserpine model of depression in rat – changes in noradrenaline and serotonin levels

72%

Mozdzen E. , Wasik A. , Michaluk J. , Romanska I. , Antkiewicz-Michaluk I.

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tom 73

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nr 1

EN

It is well known that monoamine neurotransmitters: noradrenaline (NA) and serotonin (5-HT) play a key role in central nervous system (CNS) in pathophysiology of depression. The alterations in their metabolism in the brain seem to be related to the therapeutic action of antidepressants. Abnormalities with monoaminergic storage and neurotransmission are associated with a number of neurological disorders as: e.g. depression. Reserpine, used as an antihypertensive, antipsychotic drug in a low doses is a potent inhibitor of the vesicular monoamine transporter 2 (VMAT2) and acts by depleting cells of their monoamines stores. It is known that patients who took reserpine chronically began to display symptoms similar to that seen in depression. The reserpine model of depression in rat based on universally accepted monoamine hypothesis of depression and offers good predictive validity in terms of monoamine-based antidepressant activity. The aim: the present study aimed to investigate the potential antidepressant properties of an endogenous amine 1,2,3,4-tetrahydroisoquinoline (TIQ) and its possible mechanisms of action. In behavioral study, the forced swim test (FST) was used to evaluate the effects of TIQ in reserpine model of depression in rat. Additionally, the motor function of rat was checked in locomotor activity test after investigated drugs administration. Further, the content of NA, 5-HT and their metabolites, as well as the rate of metabolism in different rat brain structures were determined by HPLC methodology with ED. The reserpine model of depression was induced by chronic (14 consecutive days) administration of reserpine in a low dose (0.2 mg/kg i.p.). Results: The results from both behavioral and neurochemical studies have shown depressive-like effect of reserpine after its chronic administration. In the behavioral tests, reserpine decreased the locomotor activity (about 30% vs. control group, P<0.05) measured in actometers (Opto-Varimex activity monitors, Columbus Instruments, USA) linked on-line to a compatible IBM-PC. 14-days administration of reserpine induced also behavioral changes in FST: increase of immobility time with a simultaneous decrease of swimming activity (about 30% vs. control group). Depressive-like action of reserpine was also observed in neurochemical study by decline NA and 5-HT levels in the brain structures, mainly in the frontal cortex and striatum. TIQ (25 mg/kg i.p.) revealed antidepressant-like effect in FST and has the ability to reverse the pro-depressive effect of reserpine. In biochemical studies, TIQ completely antagonized reserpine-induced monoaminergic depression in rat brain structures. Conclusion: The obtained data indicate first, that chronically administered reserpine at a low dose leads to a good animal model of depression. Secondly, the antidepressant-like effect of TIQ based mainly on activation of monoaminergic system and antagonizing the effect of reserpine by inhibition of MAO-dependent oxidation of monoamines and increased their concentrations in the brain. Thus, in that light TIQ may be useful as a new safer and more effective compound in clinical practice for therapy of depression.

5

A simple, non-invasive method for the measurement of reserpine-induced tremor in rats

72%

Meert T. F. , Rombouts J. , Voeten J. , Naranji F. , Clincke G.

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1997

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tom 57

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nr 1

6

NANC transmission in intestines and its pharmacological modulation

51%

Bauer V.

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tom 53

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nr 1

7

Up-regulation of spermidine-spermine N1-acetyltransferase [SSAT] expression is a part of proliferative but not anabolic response of mouse kidney

51%

Dudkowska M. , Stachurska A. , Grzelakowska-Sztabert B. , Manteuffel-Cymborowska M.

Acta Biochimica Polonica

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2002

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tom 49

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nr 4

EN

A differential expression pattern of spermidine/spermine N1-acetyltransferase (SSAT), the enzyme critical to proper homeostasis of cellular polyamines, is reported in mouse kidney undergoing hyperplasia and hypertrophy. We have shown that SSAT activity and SSAT mRNA are significantly induced by antifolate CB 3717 and folate that evoke a drug-injury-dependent hyperplasia. In contrast, SSAT activity is down-regulated in the testosterone-induced hypertrophic kidney, while SSAT mRNA is positively controlled by this androgen. Catecholamine depletion evoked by reser- pine drastically decreases the folate-induced activity of «S-adenosylmethionine decar- boxylase (AdoMetDC), which limits polyamine biosynthesis, but has no effect on SSAT activity augmented by CB 3717. Our results document that the increased SSAT expression solely accompanies the proliferative response of mouse kidney, and suggest the importance of post-transcriptional regulation to the control of SSAT activity in both hyperplastic and hypertrophic experimental models.