Medicinal plant is most exclusive sources of life saving drugs for the majority of the world population. Laboratory evaluations were made assess the study of primary metabolites of various plants in selected species Eupatorium triplinerve Vahl. During the present investigation comparative study of primary metabolites Carbohydrates, Protein, Ascorbic acid, Starch, Sugar, Chlorophyll, Carotenoids and Total phenol of genus Eupatorium triplinerve were undertaken for study and all the their chemical composition. The leaf highest amount of chlorophyll-a (4.95gm), chlorophyll-b (4.05gm), carotenoids (3.29gm),ascorbic acid (41.13gm/gdm), sugar (3.92gm/gdm), starch(5.89gm/gdm)protein (67.82µg/mg), total phenol (84.00gm/gdm). The root lowest amount of sugar (2.00gm/gdm), starch (3.15gm/gdm), protein (60.01µg/mg), ascorbic acid (43.09gm/gdm), total phenol (65.13gm/gdm).
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