Wyniki wyszukiwania - Biblioteka Nauki

1

Effect of cadmium on collagen content and solubility in rat bone.

100%

Galicka A. , Brzóska M. M. , Średzińska K. , Gindzienski A.

Acta Biochimica Polonica

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2004

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tom 51

|

nr 3

825-829

EN

The toxic action of cadmium in the bone tissue is known, but its mechanisms are still unexplained. We examined whether Cd influences collagen content and its solubility in the femoral bone of three-week-old female rats exposed to 5 or 50 mg Cd/l in drinking water. Non-cross linked collagen was extracted with 0.5 M acetic acid, and two acid-insoluble collagen fractions were extracted with pepsin and 4.0 M guanidine hydrochloride, respectively. SDS/PAGE showed the presence of two collagen types, I and V, in all three extracted fractions. Exposure of rats to Cd for 6 months increased the amount of acid-soluble collagens type I and V and decreased the level of acid-insoluble collagens. The amount of total collagen extracted from the bones of rats exposed to 50 mg Cd/l was reduced by about 14% as compared to control and those intoxicated with 5 mg Cd/l. The solubility of type I bone collagen (determined as the percentage of acetic-soluble fraction of total collagen) was increased 2.9- and 3.0-fold in rats intoxicated with 5 and 50 mg Cd/l, respectively. Similarly, the solubility of type V collagen was increased 2.3- and 2.7-fold, respectively. Our results indicate that Cd treatment affects bone collagen by decreasing its content and increasing its solubility.

2

Effect of a high amino acid diet on antioxidant barrier parameters of rat skin.

100%

Niczyporuk M. , Knaś M. , Grądzka K. , Car H.

Progress in Health Sciences

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2019

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tom 1

45-50

EN

Introduction: Oxidative stress is largely responsible for numerous skin complications that occur in the course of various diseases as well as accelerated skin aging. A high amino acid diet, supplemented with whey protein concentrate (WPC), is well-balanced and has well-absorbing proteins, which are an ideal source of essential amino acids. Purpose: To assess what changes will occur in the antioxidant barrier of unharmed skin of rats on a high amino acid diet. Materials and methods: The study was conducted on sexually mature male Wistar rats (160-180g): 1. control (standard feed), 2. high amino acid diet (WPC-80 80% whey protein) administered for 7 days at a dose of 0.3g/kg of body weight, 3. WPC-80 for 7 days at a dose of 0.5g/kg of body weight, 4. WPC-80 for 14 days at a dose of 0.3g/kg of body weight, 5. WPC-80 for 14 days at a dose of 0.5g/kg of body weight. Total antioxidant capacity, total oxidative status and oxidative stress index were determined. Results: Enrichment of a standard diet with WPC-80 did not affect the total oxidative status of undamaged healthy rat skin. This study shows that a diet rich in amino acids in rats caused an increase in total antioxidant capacity, but statistically significant values were obtained after 14 days of administering WPC at a dose of 0.5mg/kg of body weight. Conclusions: Enrichment of a standard diet with WPC-80 strengthens the antioxidant barrier in unwounded healthy rat skin.

3

Pharmacokinetics of isocorynoxeine in rat plasma after intraperitoneal administration by UPLC–MS/MS

100%

Zhan H. , Wei Z. , Ren K. , Tong S. , Wang X. , Wu Q.

Acta Chromatographica

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2020

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tom Vol. 32, no. 4

260--263

EN

Isocorynoxeine is one of the main alkaloids in Chinese medicinal herbs, and has pharmacological activities such as antihypertensive, sedative, anticonvulsant, and neuronal protection. It is an effective component of Uncaria for the treatment of hypertension. In this study, we used a fast and sensitive ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) to detect isocorynoxeine in rat plasma and investigated its pharmacokinetics in rats. Six rats were given isocorynoxeine (15 mg/kg) by intraperitoneal (i.p.) administration. Blood (100 μL) was withdrawn from the caudal vein at 5 and 30 min and 1, 2, 4, 6, 8, 12, and 24 h after administration. Chromatographic separation was achieved using a UPLC BEH C18 column using a mobile phase of acetonitrile–0.1% formic acid with gradient elution. Electrospray ionization (ESI) tandem mass spectrometry in the multiple reaction monitoring (MRM) mode with positive ionization was applied. Intra-day and inter-day precisions (relative standard deviation, %RSD) of isocorynoxeine in rat plasma were lower than 12%. The method was successfully applied in the pharmacokinetics of isocorynoxeine in rats after intraperitoneal administration. The t1/2 of isocorynoxeine is 4.9 ± 2.1 h, which indicates quick elimination.

4

Octabromodiphenyl ether - porphyrogenicity after repeated administration to rats

100%

Bruchajzer E. , Frydrych B. , Szymańska J. A.

International Journal of Occupational Medicine and Environmental Health

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2012

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tom 25

|

nr 4

392-403

EN

Objectives: Octabromodiphenyl ether (OctaBDE) is a fl ame retardant which has been withdrawn from common use due to its negative effect on the environment. The literature data regarding its toxicity addresses its effect on liver function, the endocrine and reproductive systems, as well as its developmental toxicology aspects. The aim of this study was to investigate the effect of repeated administration of OctaBDE on heme biosynthesis in rats. Materials and Methods: The study was performed on female Wistar rats. OctaBDE was administered intragastrically at four different doses (2, 8, 40 or 200 mg/kg/day) for 7, 14, 21 or 28 days. The following measures of heme synthesis disturbance were used: urinary excretion of porphyrins, liver concentration of porphyrins, the activity of delta-aminolevulinate synthase (ALA-S) and delta-aminolevulinate dehydratase (ALA-D) in the liver. Results: After 28 days of exposure, lower ALA-S and ALA-D activity was observed in the liver. Additionally, increased concentrations of high carboxylated porphyrins (octa- and heptacarboxyporphyrins) were found in the liver: from 2- to 10-fold after the 2 mg/kg/day doses and from 4- to 14-fold after the 8-200 mg/kg/day doses. The porphyrogenic effect of OctaBDE was also evidenced by augmented, dose-dependent and exposure time-dependent, concentrations of total porphyrins in urine (2-7.5-fold increase) and their urinary excretion (2-9-fold increase). Tetracarboxyporphyrins predominated in the urine; their concentrations increased 2.5-10 fold. Conclusions: The study revealed that repeated exposure to OctaBDE affects heme biosynthesis and the levels of porphyrins. The lowest effective level which induced changes in porphyrin concentration was 2 mg/kg/day.

5

Effect of a high amino acid diet on antioxidant barrier parameters of rat skin. Part 2

100%

Knaś M. , Niczyporuk M. , Grądzka K. , Car H.

Progress in Health Sciences

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2019

|

tom 1

70-75

EN

Purpose: The imbalance between the formation of reactive oxygen species and antioxidant capacity of the body is known as oxidative stress. Exposition of the skin to free radicals, the origin of the internal and external causes activation of multiple mechanisms to eliminate them and prevent in this way the development of oxidative stress. The aim of this experiment was examining what changes are taking place in the antioxidant barrier of unwounded healthy skin of rats, who are on a high amino acids diet for 7 and 14 days at administered doses of 0.3 and 0.5 g/kg body weight. Materials and Methods: The study was performed on male Wistar rats divided into 5 groups: 1. control (standard feed), 2. high amino acid diet (WPC-80 80% whey protein) administered for 7 days at a dose of 0.3g/kg of body weight, 3. WPC-80 for 7 days at a dose of 0.5g/kg of body weight, 4. WPC-80 for 14 days at a dose of 0.3g/kg of body weight, 5. WPC-80 for 14 days at a dose of 0.5g/kg of body weight. The concentration of superoxide dismutase 2 and 3, the concentration of catalase specific activity of glutathione peroxidase, the concentration of glutathione and total protein content were determined. Results: The supplementation of the standard diet by the preparation of WPC-80 administered in a dose 0.5 g/kg body weight for 14 days containing methionine and cysteine (essential amino acids involved in the formation of glutathione), significantly increases the concentration of reduced glutathione. Conclusions: Enrichment of a standard diet with WPC-80 caused by the significant increases of non-enzymatic antioxidant.

6

A preliminary study of the submandibular gland of the rat after long-term cadmium intoxication

100%

Czykier E. , Dzieciol J. , Zalewska A. , Zwierz K.

Folia Morphologica

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2003

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tom 62

|

nr 3

305-307

EN

The aim of the study was to establish to what degree the 24-week exposure of a rat to 5 and 50 mg Cd/dm³ affects the proliferating activity of cells with PCNA and Ki-67 nuclear immunoreactivity in the submandibular gland cells. The control animals received only redistilled water to drink. The group I rats were given 5 mg Cd/dm³, while the group II animals were given 50 mg Cd/dm³. The highest concentration of cadmium was observed in group II, with a concomitant increase in the number of PCNA-positive cells. In group I, cadmium concentration was significantly less compared to group II, and there were fewer PCNA-positive cells. The reaction for Ki-67 in both experimental groups was negative.

7

The effect of exposure route on the distribution and excretion of hexachloronaphthalene in rats

100%

Kilanowicz A. , Daragó A. , Skrzypińska-Gawrysiak M.

International Journal of Occupational Medicine and Environmental Health

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2012

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tom 25

|

nr 2

185-195

EN

Objectives: Polychlorinated naphthalenes (PCNs), like other persistent organic pollutants (POPs), are widespread, global environmental contaminants. These compounds still represent a great environmental problem, mostly because of the risk of secondary air pollution. They are characterized by long durability and tendency to bioaccumulate, which means that they are practically ubiquitous in all environmental media and ecosystems. The aim of this study was to investigate the distribution and excretion of hexachloronaphthalene (HxCN) in rats following a single intraperitoneal or intragastrical administration. Materials and Methods: Experiments were performed on male outbred Wistar rats with body weight of 220–240 g. They were given [¹⁴C]-HxCN intraperitoneally (i.p.) or intragastrically (p.o.) in a single dose of 0.3 mg (150 kBq) per rat. The distribution of radioactivity in blood and selected organs or tissues, as well as urine and faeces excretion were traced following the administration. Results: The decline of [¹⁴C]-HxCN in plasma was biphasic and the calculated half-lives for phases I and II were ~6 and 350 h, respectively. Following 120 h after administration, ~51% (intragastrical) and ~34% (intraperitoneal) of the dose were excreted with faeces. Regardless of the administration route, the highest HxCN concentrations were found in liver and adipose tissue, where the compound showed high retention: the highest retention in liver was found 24 h after intragastrical (32%) and intraperitoneal (38%) administration while in adipose tissue ~30% retention was observed 120 h after HxCN administration regardless of its route. Conclusions: Following the calculation of the balance of total [¹⁴C]-HxCN excreted and stored, it was found that hexachloronaphthalene belongs to the compounds of a slow turnover rate, and in the case of repeated exposure it may accumulate in the rat body.

8

Expression level of Ubc9 protein in rat tissues.

100%

Gołębiowski F. , Szulc A. , Sakowicz M. , Szutowicz A. , Pawełczyk T.

Acta Biochimica Polonica

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2003

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tom 50

|

nr 4

1064-1073

EN

Ubc9 is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. In this report we describe a simple and efficient method for obtaining pure human recombinant Ubc9 protein. The purified Ubc9 retained its native structure and was fully active in an in vitro sumoylation assay with the promyelocytic leukaemia (PML) peptide as a substrate. In order to better understand the physiology of Ubc9 protein we examined its levels in several rat tissues. Immunoblot analyses performed on tissue extracts revealed quantitative and qualitative differences in the expression pattern of Ubc9. The Ubc9 protein was present at a high level in spleen and lung. Moderate level of Ubc9 was detected in kidney and liver. Low amount of Ubc9 was observed in brain, whereas the 18 kDa band of Ubc9 was barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the Ubc9 antibodies recognized a 38 kDa protein band. This band was not visible in extracts of other rat tissues. A comparison of the relative levels of Ubc9 mRNA and protein indicated that the overall expression level of Ubc9 was the highest in spleen and lung. In spleen, lung, kidney, brain, liver and heart there was a good correlation between the 18 kDa protein and Ubc9 mRNA levels. In skeletal muscle the Ubc9 mRNA level was unproportionally high comparing to the level of the 18 kDa protein. The presented data indicate that in the rat the expression of the Ubc9 protein appears to have some degree of tissue specificity.

9

Serum Osteocalcin Concentration in Treadmill-Trained Adult Male Wistar Rats

100%

Nowak A. , Pogrzebna M. , Celichowski J. , Pilaczyńska-Szcześniak Ł.

Journal of Human Kinetics

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2008

|

tom 19

121-130

EN

Mechanical stress is considered to be essential for the regulation of bone mass. The purpose of this study was to determine whether treadmill exercise at moderate intensity induces alterations in blood osteocalcin concentration in rats. Male Wistar rats, aged 5 months, were divided randomly into two groups: trained animals (n = 6) and controls (n = 7). Trained rats were exercised 5 days/week for 4 weeks on a motor-driven treadmill. Each exercise session lasted 60 minutes and the average locomotion speed was 16.2 m/min. After completion of the training period, a blood sample was taken for osteocalcin measurement and the hindlimbs medial gastrocnemius muscles were excised and weighed. Comparative analysis showed significantly lower circulating osteocalcin levels in the exercised rats in comparison to control animals. It is possible that the observed decreased blood osteocalcin concentration is transient in nature. Factors including stress may also influenced the results.Serum osteocalcin concentration in treadmill-trained rats

10

Pterygopalatine ganglion in rat. II. Ultrastructural research

100%

Kuder T.

Folia Morphologica

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1990

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tom 49

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nr 1-2

11

Pharmacokinetic UPLC–MS/MS studies on byakangelicol after oral and intravenous administration to rats

100%

Bao X. , Huang B. , Mao Y. , Zhang Z. , Zhou Y. , Wen C. , Zhou Q.

|

tom Vol. 32, no. 1

49--52

EN

Byakangelicol is one of coumarins from Baizhi and has been shown to inhibit the release of PGE2 from human lung epithelial A549 cells in a dose-dependent manner. A sensitive ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed and full validated for the quantification of byakangelicol in rat plasma. The pharmacokinetics of byakangelicol after both intravenous (5 mg/kg) and oral (15 mg/kg) administrations were studied. Chromatographic separation was performed on an ultra-performance liquid chromatography ethylene bridged hybrid (UPLC BEH) C18 column with acetonitrile and 0.1% formic acid as the mobile phase at a flow rate of 0.4 mL/min; fargesin was used as the internal standard (IS). The following quantitative analysis of byakangelicol was utilized in the multiple reaction monitoring mode. The samples were extracted from rat plasma via protein precipitation using acetonitrile. In the concentration range of 1–2000 ng/mL, the method correlated linearity (r > 0.995) with a lower limit of quantitation (LLOQ) of 1 ng/mL. Intra-day precision was less than 11%, and inter-day precision was less than 12%. The accuracy was between 92.0% and 108.7%, the recovery was better than 89.6%, and the matrix effect was between 85.9% and 98.6%. The method was successfully applied to a pharmacokinetic study of byakangelicol after intravenous and oral administration, and the absolute bioavailability was 3.6%.

12

Validated UPLC-MS/MS method for the determination of ivosidenib in rat plasma: Application to a pharmacokinetic study

100%

Chen M. , Xu J. , Chen F. , Zhou Q. , Wang S. , Han A.

Acta Chromatographica

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2023

|

tom Vol. 35, no. 3

227--232

EN

Ivosidenib (AG-120) is an unlisted, but estimated to be valid, oral inhibitor for isocitrate dehydrogenase 1 (IDH1) in the phase Ⅰ study of IDH1-mutated acute myeloid leukemia (AML) patients. This paper presents the investigation and validation of a rapid, effective, qualitative and quantitative determination method of ivosidenib in rat plasma by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The samples were treated using acetonitrile precipitation to remove protein influence. Then, the supernatant was extracted to analyze plasma concentration traits. In the UPLC system, acetonitrile and water containing 0.1% formic acid were selected as a cosolvent mobile phase, applying a gradient elution to isolate compounds in a C18 column. Mass detections were performed on a triple quadruple mass spectrometer in positive ion mode. Electroshock characteristic fragment ionization was used for m/z 583.95→214.53 for ivosidenib for quantitative determination, m/z 583.95→186.6 for qualitative determination, and m/z 492.06→354.55 for IS. The selectivity, linearity, stability, accuracy and precision were verified by reaching the guideline criteria from European Medicine Agency (EMA) and the Food and Drug Administration (FDA). The calibration curve was linear over the concentration range of 2–2,000 ng mL⁻¹ for ivosidenib in rat plasma with a lower limit of quantification (LLOQ) of at least 2 ng mL⁻¹. Additionally, there was no distinct matrix effect or carry-over phenomenon. The method was successfully established and applied to separate ivosidenib from plasma, with the entire analytical process being performed within 3 min for each sample, which shows high-efficiency and convenience for further studies of ivosidenib.

13

Determination of diosmetin-7-o-β-d-glucoside in rat plasma by UPLC–MS/MS

100%

Li J. , Yu Z. , Han C. , Wang Z. , Hu Y. , Wen C. , Lin C.

Acta Chromatographica

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2020

|

tom Vol. 32, no. 4

264--268

EN

In this study, we used UPLC–MS/MS to determine diosmetin-7-o-β-d-glucoside in rat plasma and investigated its pharmacokinetics in rats. Six rats were given diosmetin-7-o-β-d-glucoside (5 mg/kg) by intravenous (i.v.) administration. The blood (150 μL) was withdrawn from the caudal vein after administration. Diazepam was used as an internal standard (IS), and a one-step acetonitrile precipitation method was used to process the plasma samples. Chromatographic separation was achieved using a UPLC BEH C18 column using a mobile phase of acetonitrile–0.1% formic acid with gradient elution. Electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode with positive ionization was applied, 463.1 → 301.0 for diosmetin-7-o-β-d-glucoside, m/z 285.1 → 193.0 for diazepam (IS). Intra-day and inter-day precision of diosmetin-7-o-β-d-glucoside in rat plasma were less than 14%. The method was successfully applied in the pharmacokinetics of diosmetin-7-o-β-d-glucoside in rats after intravenous administration. The t1/2 of diosmetin-7-o-β-d-glucoside is 1.4 ± 0.4 h, which indicates the quick elimination.

14

Induction of aberrant crypts in the colons of rats by alkylating agents

100%

Bilbin M. , Tudek B. , Czeczot H.

Acta Biochimica Polonica

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1992

|

tom 39

|

nr 1

15

The ultrastructure of the peritoneal membrane in chronically dialysed rats

100%

Wieczorowska-Tobis K. , Polubinska A. , Brelinska R. , Breborowicz A.

Folia Histochemica et Cytobiologica

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2001

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tom 39

|

nr 2

16

Phytosulfokines exert antinociceptive effect in rats

100%

Plech A. , Konopinska D. , Rykaczewska-Czerwinska M. , Bahyrycz A.

Pestycydy

|

2004

|

nr 3-4

17

Study on convulsive effect of meso-tetra-4N-methylpirydyl-porphyrin [P] in rats

100%

Plech A. , Maksym B. , Habdas J.

Pestycydy

|

2004

|

nr 3-4

18

Effect of insect neuropeptide leucopyrokinin [LPK] on rectal temperature in rats. Preliminary study

100%

Rykaczewska-Czerwinska M. , Lombarska-Sliwinska D. , Konopinska D. , Plech A.

Pestycydy

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2004

|

nr 3-4

19

Nuclear bodies in paneth cells of the rat

100%

Sulikowska-Rowinska A. , Rowinski J.

Folia Histochemica et Cytobiologica

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1993

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tom 31

|

nr 1

20

The course of the glucose tolerance curve in rats infected with Nippostrongylus brasiliensis

100%

Stpiczynska R.

Acta Parasitologica

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1992

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tom 37

|

nr 1

EN

The effect of Nippostrongylus brasiliensis infection (3000 larvae) on the course of the glucose tolerance curve in rats was investigated. On the 4th and 9th day after infection (DAI) rats displayed severe fasting hypoglycemia. Following oral glucose administration after 30 min this sugar appeared in blood of all rats: controls and infected ones (4 and 9 DAI) at a similar rate; however, the glucose tolerance curves of the infected rats, in contrast to controls, failed to reach maximal values after 30 min from the time of oral glucose administration. The infected and control rats exhibited an approximately similar blood glucose concentration only after 150 min from this administration. The results presented indicate that in rats infected with N. brasiliensis the glucose requirement is increased; malabsorption exerts no effext on the course of the glucose tolerance curves, and glucose deficiencies are compensated only after a lapse of a sufficiently long period (150 min) after glucose administration.