Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

Znaleziono wyników: 5

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: rapid detection

Sortuj według:

Ogranicz wyniki do:

Biological threat detection in the air and on the surface: how to define the risk

100%

Trafny E. A. , Lewandowski R. , Stepinska M. , Kaliszewski M.

nr 4

Rapid and reliable detection of Echinococcus multilocularis from faeces using droplet digital PCR

86%

Bago F. , Hoelzl F. , Knauer F. , Kubber-Heiss A. , Smith S.

2021

tom 66

nr 2

Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli

86%

Zhao L. Y. , Niu J. H. , Gao X. L. , Liu C. N. , Liu S. M. , Jiang N. , Lv X. P. , Zheng S. M. , Zhao L. Y. , Niu J. H. , Gao X. L. , Liu C. N. , Liu S. M. , Jiang N. , Lv X. P. , Zheng S. M.

nr 1

A novel, rapid, and simple PMA-qPCR method for detection and counting of viable Brucella organisms

72%

Zhang S.-J. , Wang L.-L. , Lu S.-Y. , Hu P. , Li Y.-S. , Zhang Y. , Chang H.-Z. , Zhai F.-F. , Liu Z.-S. , Li Z.-H. , Ren H.-L.

tom 64

nr 2

HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA

72%

Osinska E. , Golke A. , Slonska A. , Cymerys J. , Banbura M. W. , Dzieciatkowski T.

Polish Journal of Veterinary Sciences

2012

tom 15

nr 3

Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.