Wyniki wyszukiwania - Biblioteka Nauki

1

A novel polymorphism within intron B of growth hormone gene (GH2) of the rainbow trout, Oncorhynchus mykiss

100%

Panicz R. , Zych S. , Grzesiak W.

Polish Journal of Natural Sciences

|

2014

|

tom 29

|

nr 2

EN

Nucleotide composition of both growth hormone variants of rainbow trout (Oncorhynchus mykiss) has been strongly preserved evolutionally what might suggest that any change within these sequences can have an influence on the functioning of the somatotropic axis. A 121 bp fragment that contained nearly the entire B intron was amplified by the polymerase chain reaction. PCR products were bidirectionally sequenced. PCR products were digested by TaiI according to manufacturer’s instructions and resulting DNA was subjected to electrophoresis. An analysis of the gene fragment for growth hormone 2 showed the presence of SNP, easily identifiable by means of digestion with TaiI restriction enzyme. Statistical analysis confirmed that homozygous GHBB fish were the longest (31.77 cm) and the heaviest (404.70 g) and were statistically significantly different (P ≤ 0.05) from heterozygous GHAB fish. Mean length of GHAA homozygous fish was insignificantly lower (30.06 cm) with mean body weight of 339.12 g than homozygotes GHBB.

PL

Sekwencja nukleotydowa obu wariantów genu hormonu wzrostu pstrąga tęczowego (Oncorhynchus mykiss) jest silnie zakonserwowana ewolucyjnie. Każde nowo powstałe miejsce polimorficzne może mieć więc wpływ na funkcjonowanie osi somatotropowej. Badano niepełną sekwencję intronu B genu hormonu wzrostu typu 2 o długości 121 pz, uzyskaną po przeprowadzeniu dwukierunkowej reakcji sekwencjonowania. Otrzymane produkty PCR poddano analizie z wykorzystaniem endonukleazy TaiI, a wynik tej reakcji zobrazowano poprzez rozdział otrzymanych fragmentów restrykcyjnych w żelu agarozowym. Podczas analiz wykazano obecność mutacji punktowej, którą można łatwo zidentyfikować poprzez trawienie sekwencji nukleotydowej intronu B enzymem restrykcyjnym TaiI. W analizie statystycznej otrzymanych danych potwierdzono, że homozygotyczne GHBB pstrągi o średniej długości 31,77 cm oraz masie 404,70 g różniły się statystycznie istotnie (P ≤ 0,05) od osobników heterozygotycznych GHAB. Średnia długość (30,06 cm) oraz masa (339,12 g) homozygotycznych GHAA ryb były nieistotnie niższe od analogicznych parametrów osobników z genotypem GHBB. Scharakteryzowany polimorfizm ma istotny statystycznie wpływ na tempo wzrostu badanych osobników pstrąga tęczowego.

2

Experimental infection with epizootic haematopoietic necrosis virus (EHNV) of rainbow trout (Oncorhynchus mykiss Walbaum) and European perch (Perca fluviatilis L.)

100%

Borzym E. , Maj-Paluch J.

Bulletin of the Veterinary Institute in Puławy

|

2015

|

tom 59

|

nr 4

EN

The aim of this study was the determination of the susceptibility of Polish farmed redfin perch (Perca fluviatilis L.) and rainbow trout (Oncorhynchus mykiss Walbaum) to experimental infection with haematopoietic necrosis virus (EHNV). A bath challenge model was tested at two temperature ranges: 13-15°C and 20-22°C. After 7 d, the first clinical signs and mortality were observed in fish kept at these temperatures. Significantly more mortality cases were reported in the redfin perch population, reaching a maximum of 24% compared with 12% in the rainbow trout group at 20-22°C. EHNV was reisolated from redfin perch and rainbow trout tissue in cell culture and the infection was confirmed by a molecular method and histopathology during the duration of the experiment. This study revealed that fish from Polish farms can be susceptible to EHNV even at lower temperatures.

3

Effect of linear alkylbenzene sulfonate on some biochemical parameters in rainbow trouts [Oncorhynchus mykiss]

100%

Turkmen G.

Medycyna Weterynaryjna

|

2004

|

tom 60

|

nr 05

4

Preparation and anatomical distribution study of 67Ga-alginic acid nanoparticles for SPECT purposes in rainbow trout (Oncorhynchus mykiss)

86%

Heidarieh M. , Daryalal F. , Mirvaghefi A. , Rajabifar S. , Diallo A. , Sadeghi M. , Zeiai F. , Moodi S. , Maadi E. , Sheikhzadeh N. , Heidarieh H. , Hedyati M.

Nukleonika

|

2014

|

tom Vol. 59, No. 4

153--159

EN

Ergosan contains 1% alginic acid extracted from two brown sea weeds. Little is known about the target organs and anatomical distribution of Ergosan (alginic acid) in fi sh. Therefore, feasibility of developing alginic acid nanoparticles to detect target organ in rainbow trout is interesting. To make nanoparticles, Ergosan extract (alginic acid) was irradiated at 30 kGy in a cobalt-60 irradiator and characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results from TEM images showed that particle sizes of irradiated alginic acid ranged from 30 to 70 nm. The FTIR results indicated that gamma irradiation had no signifi cant infl uence on the basic structure of alginic acid. Later, alginic acid nanoparticles were successively labelled with 67Ga-gallium chloride. The biodistribution of irradiated Ergosan in normal rainbow trout showed highest uptake in intestine and kidney and then in liver and kidney at 4- and 24-h post injection, respectively. Single-photon emission computed tomography (SPECT) images also demonstrated target specifi c binding of the tracer at 4- and 24-h post injection. In conclusion, the feed supplemented with alginic acid nanoparticles enhanced SPECT images of gastrointestinal morphology and immunity system in normal rainbow trout.

5

Preparation and anatomical distribution study of67Ga-alginic acid nanoparticles for SPECT purposes in rainbow trout (Oncorhynchus mykiss)

86%

Heidarieh M. , Daryalal F. , Mirvaghefi A. , Rajabifar S. , Diallo A. , Sadeghi M. , Zeiai F. , Moodi S. , Maadi E. , Sheikhzadeh N. , Heidarieh H. , Hedyati M.

Nukleonika

|

2014

|

tom 59

|

nr 4

153-159

EN

Ergosan contains 1% alginic acid extracted from two brown sea weeds. Little is known about the target organs and anatomical distribution of Ergosan (alginic acid) in fish. Therefore, feasibility of developing alginic acid nanoparticles to detect target organ in rainbow trout is interesting. To make nanoparticles, Ergosan extract (alginic acid) was irradiated at 30 kGy in a cobalt-60 irradiator and characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results from TEM images showed that particle sizes of irradiated alginic acid ranged from 30 to 70 nm. The FTIR results indicated that gamma irradiation had no significant influence on the basic structure of alginic acid. Later, alginic acid nanoparticles were successively labelled with 67Ga-gallium chloride. The biodistribution of irradiated Ergosan in normal rainbow trout showed highest uptake in intestine and kidney and then in liver and kidney at 4- and 24-h post injection, respectively. Single-photon emission computed tomography (SPECT) images also demonstrated target specific binding of the tracer at 4- and 24-h post injection. In conclusion, the feed supplemented with alginic acid nanoparticles enhanced SPECT images of gastrointestinal morphology and immunity system in normal rainbow trout.

6

Methane Production from Farmed Rainbow Trout Byproducts by Anaerobic Digestion in Morocco

86%

Essalhi F. , Bengueddour R.

Ecological Engineering & Environmental Technology

|

2023

|

tom Vol. 24, iss. 2

45--54

EN

Fish are a staple in human nutrition because of their great nutritional and dietetic value. The processing stages of these fish before being sold generates an important quantity of byproducts (head, viscera, skeleton, fins, scales, tail, and skin). This study aims to determine the methane production, methanogenic potential and the percentage of volatile solids removed by the anaerobic digestion process. The experiment was carried out at mesophilic conditions in a Continuous Stirred-Tank Reactor (CSTR) with a discontinuous feeding mode (batch). The results obtained showed that the byproducts of farmed rainbow trout have a maximum methane production 1176 Nml whose methanogenic potential is 206.68 Nml/gVS with a biodegradability equal to 57.95%. For the kinetic modeling, four models were applied for an adequate fit of the experimental results (first order, MGompertz, transfererence function, and logistic function). The production of methane closest to the experimental results is that estimated by the logistic function with a methanogenic potential of 212.21 Nml/gVS whose correlation coefficient R2= 0.9870 and a very low percentage of error (1.18%), also the MGompertz model presented results adapted to those of the experiment with a methanogenic potential equal to 223.61 Nml/gVS (R2= 0.9889 and %error = 2.95); This confirms that these two kinetic models are the most suitable for this type of substrate.

7

Observation of red blood cell alterations in triploid rainbow trout [Oncorhynchus mykiss]

86%

Johari S. A. , Kalbassi M. R. , Sourinezhad I. , Wlasow T.

|

2008

|

tom 07

|

nr 1-4

49-52

8

Use of complementary feeding stuff Ichtio Hexan to control Spironucleus salmonis infections in rainbow trout

86%

Puk K. , Guz L. , Sopinska A.

|

tom 70

|

nr 10

EN

The aim of this study was to determine the efficacy of Ichtio Hexan (a complex blend of Allium sativum extract, Chelidonium majus extract, Origanum vulgare extract, carvacrol and cinnamic aldehyde) for the control of spironucleosis (hexamitosis) in rainbow trout fingerlings. For this purpose, three groups of rainbow trout naturally infected with S. salmonis were fed diets containing either no Ichtio Hexan or supplemented with Ichtio Hexan at a rate of 0.1 ml/kg bw (group 1) or 1 ml/kg bw (group 2) for 38 days. During this period mortalities were recorded and the dead fish were examined to confirm the isolations of S. salmonis. At the end of the experiment the fish were sacrificed, individually weighed and measured, their livers removed and weighed. In order to determine the prevalence and intensity of infection the intestinal contents were examined. The results of the present study showed that Ichtio Hexan in an amount of 0.1 ml/kg bw considerably reduced mortality caused by S. salmonis in rainbow trout fingerlings. The number of S. salmonis trophozoites in the digestive tract after administration of Ichtio Hexan at this dose for 38 days was significantly reduced and limited only to the posterior part of the intestine. On the other hand, in the 1 ml/kg bw fed group none of the fish were infected and the gain in the body weight was significantly increased; however, the mortality rate was similar to the control group. The experimental groups did not differ significantly from each other with regards to the condition factor and hepatosomatic index. Considering the above findings, Ichtio Hexan at a dose of 0.1 ml/kg bw can be successfully used in rainbow trout farms to reduce the mortality rate in S. salmonis infected fish.

9

Effect of sex reversal in rainbow trout (Oncorhynchus mykiss Walbaum) using 17alpha-methyltestosterone and 11beta-hydroxyandrostenedione

86%

Kuzminski H.

Archives of Polish Fisheries

|

2010

|

tom 18

|

nr 1

10

New multiplex PCR assays for estimating genetic diversity in rainbow trout (Oncorhynchus mykiss) by polymorphism of microsatellite DNA

86%

Kaczmarczyk D. , Kaczor A.

Environmental Biotechnology

|

2013

|

tom Vol. 9, No. 1

19--24

EN

Multiplex PCR is a useful technique for estimating genetic diversity. This paper presents 3 new sets of primer pairs for effectively amplifying 10 microsatellite DNA loci from rainbow trout (Oncorhynchus mykiss). Unlike other sets of primer pairs that have been developed for amplifying rainbow trout microsatellite loci, ours do not require the hot-start PCR technique. In the paper, we describe the steps taken to choose the loci for each multiplex assay and to verify the genotyping results. We provide the compositions of the PCR mixture and the characteristics of the PCR thermal profile recommended for amplification.

11

STAT, a comprehensive statistical analysis software as a tool for determining changes in individual weight of rainbow trout [Oncorhynchus mykiss Walb.] cultured in seawater

86%

Wawrzyniak W.

Zeszyty Naukowe. Acta Biologica. Uniwersytet Szczeciński

|

1999

|

tom 05

12

Carotenoid contents in the body of rainbow trout Oncorhynchus mykiss, from different habitats

86%

Wozniak M.

|

2000

|

tom 27

13

Effects of selected external parameters on changes in individual weight of the rainbow trout [Oncorhynchus mykiss Walb.] Kept in a sea-water culture, as determined with the 'Korelacje' computer package

86%

Wawrzyniak W.

Zeszyty Naukowe. Acta Biologica. Uniwersytet Szczeciński

|

1999

|

tom 05

14

Factors influencing spawning season and ovulation time in rainbow trout [Oncorhynchus mykiss]

86%

Goryczko K.

Polskie Archiwum Hydrobiologii

|

1997

|

tom 44

|

nr 1-2

15

Heads or tails - fish hatching

72%

Lugowska K. , Sarnowski P.

Acta Ichthyologica et Piscatoria

|

2011

|

tom 41

|

nr 1

EN

Background. Hatching is a process in which the embryo emerges from the egg by breaking the protective egg shell. Our preliminary observations indicate that hatching duration and time-distribution may vary among and within the fish species, and that some embryos fail to hatch or hatch incompletely, probably due to the “incorrect” hatching way. So the aim of this study was a detailed description of hatching of three fish species: common carp, barbel, and rainbow trout. Materials and methods. Three species of fish: common carp, Cyprinus carpio; barbel, Barbus barbus; and rainbow trout, Oncorhynchus mykiss; were examined for their hatching modes. The mode and the time of hatching of each larva were noted. Newly hatched larvae were counted and examined. The malformations were classified. Observations of embryos and larvae were done using the stereoscopic microscope Nikon connected to the computer with the MultiScan 8.4 image analysis system; the hatching embryos and larvae were photographed. Results. Three modes of hatching were observed, two of them similar in all three fish species. Some fish started hatching tail first from the egg shell, others head first or—specifically for barbel—yolk sac first. The data obtained in the present study showed that tail hatching was the most successful in all fish species, and shown by most good quality larvae. The majority of tail-hatched larvae developed normally and were viable, and only some of them were deformed and showed slight morphological defects, mainly single vertebral malformations that in most cases were negligible. Head hatching was the precarious in carp, and in all fish species less common and successful comparing to the tail hatching. Conclusion. The hatching mode could be used as another good parameter for estimation of quality of eggs and larvae.

16

Chromosome rearrangements and survival of androgenetic rainbow trout (Oncorhynchus mykiss)

72%

Ocalewicz K. , Dobosz S. , Kuzminski H. , Nowosad J. , Goryczko K.

Journal of Applied Genetics

|

2010

|

tom 51

|

nr 3

EN

The purpose of this work was to quantify the impact of spontaneous and X-radiation-induced chromosome rearrangements on survival rate of androgenetic rainbow trout (Oncorhynchus mykiss). Various doses of X irradiation (50, 150, 250, 350 Gy) were used for inactivation of nuclear DNA in oocytes. After the irradiation, eggs were inseminated with normal sperm from 4 males derived from a strain characterized by Robertsonian rearrangements and length polymorphism of the Y chromosome. The haploid zygotes were exposed to a high hydrostatic pressure (7000 psi) to duplicate the paternal DNA. Neither Robertsonian chromosome polymorphism nor the Y chromosome morphology impaired the viability of the androgenetic embryos and alevins. Moreover, survival of eyed embryos of the androgenetic rainbow trout increased significantly with increasing doses of oocyte X irradiation. After 6 months of rearing, only specimens from the 250 and 350 Gy variants survived. The number of fingerlings with remnants of the maternal genome in the forms of chromosome fragments was higher in the 250 Gy group. Intraindividual variation of chromosome fragment number was observed, and some individuals exhibited haploid/diploid mosaicism and body malformations. Individuals irradiated with less than 250 Gy died, presumably because of the conflict between intact paternally derived chromosomes and the residues of maternal genome in the form of chromosome fragments.

17

Alterations in selected haematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to complex mixtures of contaminants

72%

Vosyliene M. Z. , Kazlauskiene A. , Mikalajune A.

Environment Protection Engineering

|

2010

|

tom Vol. 36, nr 4

95-104

EN

The aim of the study was to evaluate and compare changes in the selected haematological parameters of rainbow trout after long-term (14 days) exposure to sublethal concentrations of two kinds of contaminants: 1) highway traffic emission pollutants (model mixture of heavy metals) and 2) road maintenance salt. Four groups of fish exposed to sublethal concentrations of model mixture metal solutions demonstrated an increased frequency of abnormal erythrocytes and elevated percentage of neutrophiles. The percentage of juvenile and abnormal erythrocytes was significantly increased in the blood of fish exposed to sublethal concentrations of road maintenance salt solutions. Our data confirm that even low amounts of metals found in soil near roadsides and road maintenance salt can induce adverse effects on fish. Morphological analysis of erythrocytes seems to be a reliable tool for detection of toxic effects upon fish.

18

Does hydrogen-rich water mitigate MP toxicity in rainbow trout (Oncorhyncus mykiss)? Monitoring with hematology, DNA damage, and apoptosis via ROS/GSH/MDA pathway

72%

Atamanalp M. , Kırıcı M. , Köktürk M. , Kirici M. , Alwazeer D. , Kocaman E. M. , Ucar E. , Parlak V. , Özcan S. , Alak G.

Oceanological and Hydrobiological Studies

|

2023

|

tom Vol. 52, No. 2

206--220

EN

Although the number of studies documenting the presence of Microplastics (MP) in fish is increasing, research studies focused on its detoxification are very limited. In this study, rainbow trout ( Oncorhyncus mykiss) were randomly divided into two groups after being fed with MPs (15% polypropylene [PP] +15% polyethylene [PE]) for 2 months. MP excretion without any application (PP+PE) in group I fish, and excretion of MPs with hydrogen-rich water (HRW) application (PP+PE+HRW) in group 2 were investigated under semi-static conditions for 21 days. This effectwas also compared by using positive and negative control groups (Control[no treatment, free PP, PE or/and HRW] and only HRW group). In thisdirection, the following were determined: PP+PE chronic toxicity in aquatic organisms, the toxicity mechanism and the effect of HRW as a possibletreatment method in blood tissue; with hematological indices ([RBC count [RBC], leukocyte count [WBC], hemoglobin value [Hb], hematocrit ratio [Hct], platelet count [PLT], hemoglobin count per erythrocyte [MCHC],mean hemoglobin amount per erythrocyte [MCH] and mean erythrocyte volume [MCV]) in other tissues (liver, gill and brain tissue) oxidative stress response (catalase [CAT]), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), glutathione (GSH), reactive oxygen products (ROS), malondialdehyde ([MDA] levels), DNA damage (8-OHdG: 8-Hydroxy-2-Deoxyguanosine), and the apoptosis (caspase 3) levels were investigated. In addition, acetylcholinesterase enzyme (AChE) activity, which is important in neurotoxicity pathways in the brain, wasdetermined. The presence of plastics (PP/PE) in target tissues (muscle, liver,gill and gastrointestinal tract) was also obtained.The results showed that PP+PE caused toxicity in all three tissues. MPs showed an inhibiting effect on antioxidant enzyme activities and an inductive effect on MDA, ROS, 8-OHdG, and caspase 3 levels. HRW showed a mitigating effect on MP-mediated toxicity in O. mykiss brain, blood, gill, and liver by controlling the ROS/ GSH/MDA pathway. HRW can be suggested as a cost-effective and eco-friendly curative for the protection of fish from the oxidative damages produced by the ingestion of microplastics.

19

Application of spermatozoa from neo-males (XX) to induce androgenetic development in rainbow trout (Oncorhynchus mykiss)

72%

Michalik O. , Kowalski R. , Ziomek E. , Hliwa P. , Mieszkowska A. , Rozynski R. , Ocalewicz K.

Folia Biologica

|

2017

|

tom 65

|

nr 2

20

Oxidative stress biomarkers in the heart and liver of rainbow trout (Oncorhynchus mykiss Walbaum) upon disinfection by formalin

72%

Tkachenko H. , Grudniewska J.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

|

2017

|

tom 21

EN

To evaluate the effect of formalin disinfection on the oxidative stress biomarkers in the cardiac and hepatic tissue, rainbow trout (Oncorhynchus mykiss Walbaum) were assigned into test and control groups. The test group was disinfected by formalin in final concentration 200 mL per m3. Fish were bathed for 20 min, three times, every 3 days. Two days after the last bathing fish were sampled. 2-Thiobarbituric acid reactive substances (TBARS) and carbonyl derivatives of protein oxidative destruction, as well as antioxidant defense biomarkers (superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase activity, total antioxidant capacity) were determined. The formaldehyde- exposed animals showed decrease of aldehydic and ketonic derivatives of oxidatively modified proteins and increased superoxide dismutase activity in the hepatic tissue compared to untreated group. In cardiac tissue, TBARS level, aldehydic and ketonic derivatives of oxidatively modified proteins were increased in formalin-exposed trout and down-regulated antioxidant status versus control group. It could be concluded that the disinfection of rainbow trout by formalin not contributed to the hepatic injury and may not be toxic. Toxic effect to cardiac tissue was more considerable.

PL

Celem badań była ocena wpływu dezynfekcji z formaliną na poziom biomarkerów stresu oksydacyjnego w tkance sercowej i wątrobowej pstrąga tęczowego (Oncorhynchus mykiss Walbaum). Badaną grupę ryb dezynfekowano formaliną w końcowym stężeniu 200 ml/m3. Ryby były kąpane przez 20 minut, trzykrotnie, w trzydniowych odstępach. Dwa dni po trzeciej kąpieli tkanki sercową i wątrobową pobrano do badań biochemicznych. Określono poziom biomarkerów stresu oksydacyjnego (produktów reagujących z kwasem 2-tiobarbiturowym (TBARS) i karbonylowych pochodnych oksydacyjnie zmodyfikowanych białek) oraz obrony antyoksydacyjnej (aktywności dysmutazy ponadtlenkowej, katalazy, reduktazy glutationowej, peroksydazy glutationowej, całkowitej zdolności antyoksydacyjnej). Grupa ryb po dezynfekcji z formaliną wykazała zmniejszenie poziomu aldehydowych i ketonowych pochodnych oksydacyjnie zmodyfikowanych białek oraz wzrost aktywności dysmutazy ponadtlenkowej w tkance wątrobowej w porównaniu z grupą kontrolną. W tkance sercowej dezynfekowanych ryb zanotowano zwiększenie poziomu TBARS, aldehydowych i ketonowych pochodnych oksydacyjnie zmodyfikowanych białek. Można stwierdzić, że dezynfekcja pstrąga tęczowego nie przyczynia się do uszkodzenia wątroby. Toksyczny wpływ na tkankę sercową był bardziej znaczący.