Wyniki wyszukiwania - Biblioteka Nauki

1

High-performance thin-layer chromatographic analysis of psoralen in marketed formulations and manufactured solid lipid nanoparticles (SLNs): Validation of the method

100%

Akhtar N. , Faiyazuddin M. , Mustafa G. , Sultana Y. , Baboota S. , Ali J.

Acta Chromatographica

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2012

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tom Vol. 24, no. 4

603--613

EN

A quantitative method using precoated silica gel-60 Lichrosphere high-performance thin-layer chromatography (HPTLC) plates, automated band wise sample application, and n-hexane:acetone:formic acid (2:1:0.025 υ/υ/υ) as mobile phase, has been developed and validated for the analysis of psoralen in marketed formulations and novel solid lipid nanoparticles (SLNs). Densitometric analysis was performed at 250 nm in absorbance mode. Compact bands of psoralen were obtained at Rf 0.32 ± 0.02. The method was validated for linearity, precision, robustness, sensitivity, specificity, and recovery. Linearity (r2 = 0.995), limit of detection (8.0 ng band-1), limit of quantification (18.1 ng band) -1, recovery (98.06–99.64%), and precision (≤0.74) were satisfactory. Statistical analysis established that the developed method for quantification of psoralen in marketed formulation and from solid lipid nanoparticles is reproducible and selective.

2

Photoaddition of psoralens to phosphatidylcholine in micelles. Cell membrane, a target for PUVA therapy

86%

Waszkowska E. , Poznanski J. , Zaremba Z.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

3

Isolation of furanocoumarins from Pastinaca sativa L. callus culture

86%

Ekiert H. , Kisiel W.

Acta Societatis Botanicorum Poloniae

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2000

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tom 69

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nr 3

4

Furanokumaryny : naturalne związki organiczne o wielu właściwościach

81%

Piechocka J. , Chwatko G. , Owczarek P.

Wiadomości Chemiczne

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2023

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tom [Z] 77, 9-10

937--949

EN

Psoralen and angelicin are organic, natural chemical compounds, belonging to the furanocoumarin group, which are quite common in nature. Among others, these compounds are present in plants of the celery, rutaceae, mulberry and bean families, such as celery, carrot, lemon and fig. Interestingly, they exhibit a variety of biological activities, for instance insecticidal, anticonvulsant, anticancer, and antioxidant properties. For this reason, they are commonly used in different area of life. For example, psoralen is used in photochemotherapy as a photosensitizer in the treatment of psoriasis or acquired vitiligo (PUVA therapy). However, there is evidence that overexposure to psoralen and angelicin can also cause adverse effects. For these reasons, a number of analytical methods have been developed for their determination in environmental samples, while the vast majority of them are based on separation techniques. This paper discusses issues related to psoralen and angelicin and their analytics.

5

Apoptosis induced by membrane damage in human lymphocytes; effects of arachidonic acid and its photoproducts

58%

Zarebska Z. , Zielinska J. , Zhukov I. , Maslinski W.

Acta Biochimica Polonica

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2005

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tom 52

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nr 1

EN

The effect of arachidonic acid (AA) combined with UVA irradiation was studied in a model system mimicking phototherapy PUVA (psoralen+UVA) ex vivo in vitro. The contribution of damage to the plasma membrane by PUVA was tested on human lymphocytes derived from healthy donors. The effect of arachidonic acid (AA) combined with UVA irradiation was compared with that of a psoralen photoadduct to AA added to the culture. The adduct, obtained photochemically and purified, was characterized by NMR and MS spectrometry as a cycloadduct of psoralen to the vinylene bond of the acid (AA<>PSO). The reactions of cultured cells, manifested 20 h after treatment by changes in apoptosis and mitochondrial depolarization, were monitored by flow cytometry by tagging lymphocytes with appropriate fluorescent probes. Treatment of lymphocyte suspension within AA doses from 40 to 100 μM gradually induced a shift from Anx-V+ (single positive cells) to late apoptotic, Anx-V+PI+ (double positive cells) in a dose dependent manner. The adduct, AA<>PSO, induced apoptotic changes at a concentration 2–3 times higher than free AA. Combination of psoralen (1 μM ) or arachidonic acid (20–120 μM) with UVA irradiation (2–6 J/cm2) accelerated the plasma membrane changes in a synergic way. Preliminary studies indicated that changes in the transmembrane potential of mitochondria paralleled the apoptosis when cells were treated by AA alone. Our findings showed that UVA radiation of lymphocytes in the presence of arachidonic acid, as in the presence of psoralen, enhanced apoptosis of cells in a synergic manner. Thus, PUVA-induced apoptosis may proceed in part by a still undefined signaling pathway(s) triggered in lymphocyte membranes.

6

Psoralen photosensitization: damages to nucleic acid and membrane lipids components

58%

Caffieri S. , Zarebska Z. , Dall'Acqua F.

Acta Biochimica Polonica

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1996

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tom 43

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nr 1

EN

For decades the therapeutic properties of psoralens were related to their photochemical reactions with DNA and RNA. Such approach, although fruitful in treatments, did not explain satisfactorily the way of healing of the multitude of diseases manifested through skin disorders. The new research field presented in our review is directed to another target: the lipid components of the cell. The studies on the photobiology of phospholipids may help to elucidate the phototoxic and pigment inducing activity of psoralens.

7

Problem obecnosci w zywnosci powstajacych naturalnie oraz tworzacych sie w czasie procesow technologicznych zwiazkow rakotworczych

36%

Nabrzyski M. , Ganowiak Z.

Roczniki Państwowego Zakładu Higieny

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1992

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tom 43

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nr 3-4

223-233

PL

W oparciu o przegląd piśmiennictwa światowego przedstawiono aktualny stan wiedzy na temat związków muta-, kancero- i teratogennych występujących jako składniki naturalne w żywności, jak i powstających w środkach spożywczych w następstwie szeregu zabiegów kulinarnych i technologicznych. Zwrócono uwagę, że związki te mogą stanowić poważny czynnik ryzyka zachorowań ludzi na chorobę nowotworową, stanowiąc w tej grupie przyczynę od 10 do 70% zgonów. Z kolei ryzyko zachorowań na choroby nowotworowe ze strony tak obecnie powszechnie stosowanych substancji obcych, celowo dodawanych do żywności może być przyczyną mniej niż 1% zgonów.

EN

The current opinion on the spontaneously occuring or evoked by certain culinary and technological processes muta-, cancero- and teratogenic compounds are reviewed on the basis of the world literature. It was noted that these substances may form in food products of vegetal origin. Formation of carcinogenic compounds is more intensive in plants attacked by various diseases, insects or parasites. The importance of this problem should be stressed together with the need of improving knowledge in this field. The compounds under discussion are the most serious risk factor in the incidence of neoplasms in humans.