Wyniki wyszukiwania - Biblioteka Nauki

1

Degradacja enzymatyczna bakteriobójczych kompozytów polilaktydowych

100%

Richert A. , Olewnik-Kruszkowska E. , Adamska E.

Przemysł Chemiczny

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2017

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tom T. 96, nr 12

2519--2521

PL

Zaprezentowano bakteriobójcze kompozyty polilaktydowe zawierające granulat poliheksametyloguanidyny i wosk polietylenowy pod kątem zmian struktury powierzchni i skaningowej kalorymetrii różnicowej zachodzących pod wpływem enzymu hydrolitycznego, proteinazy K.

EN

Polylactide was filled with the polyhexamethylene guanidine wax (0.2-1.0% by mass) and studied for enzymatic degrdn. in proteinase K. The addn. of the wax resulted in decreasing the crystallinity and glass transition temp.

2

Technologia produkcji pozakomórkowych proteinaz bakterii z rodzaju bacillus

100%

Trzmiel T.

Zeszyty Naukowe. Chemia Spożywcza i Biotechnologia / Politechnika Łódzka

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1998

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tom Z. 59

15-28

PL

Omówiono technologiczne aspekty związane z produkcją pozakomórkowych proteinaz bakterii z rodzaju Bacillus. Szczególną uwagę zwrócono na skład podłoży i warunków hodowli tych bakterii w procesie biosyntezy proteinaz oraz metody wyodrębniania enzymu z cieczy pohodowlanych.

EN

In the article autor attempts to give the most essential informations for production of proteinases from the Genus Bacillus. Particular attention is given to growing medium and cultivationb conditions for bacteria. The paper also describes possible pathways of proteinases isolation from cultivation medium and various procedures of their preparing.

3

Role of biochemical factors in the pathogenesis of keratoconus

100%

Wojcik K. , Blasiak J. , Szaflik J. , Szaflik J.

Acta Biochimica Polonica

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2014

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tom 61

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nr 1

55-62

EN

Keratoconus (KC) is a corneal disease associated with structural abnormalities in the corneal epithelium, Bowman's layer and stroma and altered concentration of tear components. KC corneas show a different pattern of collagen lamellae than their normal counterparts. Also, a reduction of several collagen types in KC epithelium and stroma was observed. Altered expression and/or activity of lysyl oxidase, a critical enzyme of the biogenesis of connective tissue detected in KC corneas, may weaken covalent bonds between collagen and elastin fibrils, what may lead to biomechanical deterioration of the cornea. Increased activity of matrix metalloproteinases observed in KC may induce the degradation of the extracellular matrix causing damage to the cornea. Oxidative and nitrative stress play an important role in KC pathogenesis and KC corneas are characterized by the disturbed lipid peroxidation and nitric oxide pathways. Malfunctioning of these pathways may lead to accumulation of their toxic by-products inducing several detrimental effects, along with apoptosis of the corneal cells, which may result from the loss of β-actin or increased levels of cytokines, including interleukin-1 and -6. Change in the expression of genes associated with wound healing, including the nerve growth factor and the visual system homeobox 1, may contribute to increased susceptibility of KC corneas to injury. Consequently, biochemical changes may play an important role in KC pathophysiology and, therefore, can be considered in prevention, diagnosis, prognosis and in the therapy of this disease as well.

4

Proteazy: znaczenie, rola i oznaczanie

84%

Tokarzewicz A. , Gorodkiewicz E.

Chemik

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2015

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tom Vol. 69, nr 2

81--88

PL

Proteazy są związkami, które odgrywają bardzo istotną rolę w ludzkim organizmie. Regulują one wiele procesów przebiegających także w innych żywych organizmach, również w wirusach, bakteriach i pasożytach. Ich zwiększona lub obniżona ilość może wskazywać na nieprawidłowości w orgaznizmie, takie jak: rozwój zapalenia, nowotwory, nadciśnienie. W artykule opisano najistotniejsze z ludzkich proteaz, ich znaczenie, rolę w organizmie oraz pokrótce metody oznaczania.

EN

Proteases are compounds that play an important role in the human body. They regulate many processes inside the living organisms, also in viruses, bacteria and parasites. Their increased or decreased level may indicate irregularities in the body, such as: the development of inflammation, cancer and others like hypertension. This article describes the most important of human proteases, their significance, roles in the organism and shortly methods of determination.

5

Pozakomórkowe proteinazy bakterii z rodzaju Bacillus

84%

Trzmiel T.

Zeszyty Naukowe. Chemia Spożywcza i Biotechnologia / Politechnika Łódzka

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1998

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tom Z. 58

29-47

PL

Opisano klasyfikację, charakterystykę oraz właściwości proteinaz wytwarzanych przez bakterie z rodzaju Bacillus. Szczególną uwagę poświęcono kontroli i mechanizmowi ich biosyntezy a także ich fizjologicznej roli w procesie sporulacji bakterii Bacillus.

EN

Classification, characterization and properties of the proteinases synthesizes by the Genus Bacillus are reviewed. Particular attention is given to the control and mechanisms of their synthesis. Physiological functions of proteinases in spoluration are also described.

6

Molecules released by helminth parasites involved in host colonization

67%

Dzik J. M.

Acta Biochimica Polonica

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2006

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tom 53

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nr 1

33-64

EN

Parasites are designed by evolution to invade the host and survive in its organism until they are ready to reproduce. Parasites release a variety of molecules that help them to penetrate the defensive barriers and avoid the immune attack of the host. In this respect, particularly interesting are enzymes and their inhibitors secreted by the parasites. Serine-, aspartic-, cysteine-, and metalloproteinases are involved in tissue invasion and extracellular protein digestion. Helminths secrete inhibitors of these enzymes (serpins, aspins, and cystatins) to inhibit proteinases, both of the host and their own. Proteinases and their inhibitors, as well as helminth homologues of cytokines and molecules containing phosphorylcholine, influence the immune response of the host biasing it towards the anti-inflammatory Th2 type. Nucleotide-metabolizing enzymes and cholinesterase are secreted by worms to reduce inflammation and expel the parasites from the gastrointestinal tract. An intracellular metazoan parasite, Trichinella spiralis, secretes, among others, protein kinases and phosphatases, endonucleases, and DNA-binding proteins, which are all thought to interfere with the host cellular signals for muscle cell differentiation. Secretion of antioxidant enzymes is believed to protect the parasite from reactive oxygen species which arise from the infection-stimulated host phagocytes. Aside from superoxide dismutase, catalase (rarely found in helminths), and glutathione peroxidase (selenium-independent, thus having a poor activity with H2O2), peroxiredoxins are probably the major H2O2-detoxifying enzymes in helminths. Secretion of antioxidant enzymes is stage-specific and there are examples of regulation of their expression by the concentration of reactive oxygen species surrounding the parasite. The majority of parasite-secreted molecules are commonly found in free-living organisms, thus parasites have only adapted them to use in their way of life.