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1
Content available PROTEIN DELIVERY BY NANOPARTICLES FORMED BY CHITOSAN-N-ACYL DERIVATIVES
100%
Zubareva A. А. , Ilyina A. V. , Levov А. N. , Zueva V. S. , Svirshchevskaya Е. V. , Varlamov V. P.
Progress on Chemistry and Application of Chitin and its Derivatives
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2011
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tom 16
61 - 70
EN
Biodegradable nanoparticulated carriers have important potential applications for administration of therapeutic proteins and peptides. Chitosan based nanoparticles have attracted attention due to their biological properties such as biodegrability and biocompatibility. However, chitosan nanoparticles are not stable in colloid form. Therefore, to increase the stability of these particles is an important problem. The aim of this work was to obtain stable nanoparticles formed by N-acyl derivatives of chitosan by ionic gelation and to investigate their ability to interact with acidic, neutral and basic proteins.
2
Content available remote Certain protein transducing agents convert translocated proteins into cell killers
88%
Tcherniuk S. , Fiser A.-L. , Derouazi M. , Toussaint B. , Wang Y. , Wojtal I. , Kondo E. , Szolajska E. , Chroboczek J.
Acta Biochimica Polonica
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2012
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tom 59
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nr 3
433-439
EN
The majority of proteins are unable to translocate into the cell interior. Hence for peptide- and protein-based therapeutics a direct intracytoplasmic delivery with the aid of transducing agents is an attractive approach. We wanted to deliver to the cell interior a putatively cytotoxic protein VPg. Protein transduction was achieved in vitro with three different commercial products. However, in our hands, delivery of various control proteins without known deleterious effects, as well as of protein VPg, always induced cell death. Finally, we used a novel transducing peptide Wr-T, which was not toxic to cultured cells, even in a quite large range of concentrations. Most importantly, control protein delivered to cells in culture did not display any toxicity while VPg protein exerted a strong cytotoxic effect. These data show that results obtained with cell-penetrating agents should be interpreted with caution.
3
Certain protein transducing agents convert translocated proteins into cell killers
63%
Tcherniuk S. , Fiser A.-L. , Derouazi M. , Toussaint B. , Wang Y. , Wojtal I. , Kondo E. , Szolajska E. , Chroboczek J.
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nr 3
EN
 The majority of proteins are unable to translocate into the cell interior. Hence for peptide- and protein-based therapeutics a direct intracytoplasmic delivery with the aid of transducing agents is an attractive approach. We wanted to deliver to the cell interior a putatively cytotoxic protein VPg. Protein transduction was achieved in vitro with three different commercial products. However, in our hands, delivery of various control proteins without known deleterious effects, as well as of protein VPg, always induced cell death. Finally, we used a novel transducing peptide Wr-T, which was not toxic to cultured cells, even in a quite large range of concentrations. Most importantly, control protein delivered to cells in culture did not display any toxicity while VPg protein exerted a strong cytotoxic effect. These data show that results obtained with cell-penetrating agents should be interpreted with caution.
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