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EN
These investigations aimed to evaluate the influence of acaricides used in Bulgaria (based on amitraz, cumaphos and fluvalinate) on some factors of the non-specific bee immunity in the appearance of chalkbrood. It was found that the changes of protein concentration and lysozyme amount in the haemolymph and the pH of bee intestine depend on different active substances of the drugs. Field trials canied out on 526 colonies revealed that the lower resistance of bees infected with Varroa jacobsoni was a cause of the appearance of chalkbrood. This resistance was additionally decreased by using the anti-Varroa mite product “Perizin”.
EN
Background. The distillery stillage is a major and arduous byproduct generated during ethanol production in distilleries. The aim of this study was to evaluate the possibility of the stillage recirculation in the mashing process of triticale for non-byproducts production and reducing the fresh water consumption. The number of recirculation cycles which can be applied without disturbances in the ethanol fermentation process was investigated. MateriaL and methods. Winter triticale BOGO and “Ethanol Red” Saccharomyces cerevisiae yeast were used in the experiments. The method of non-pressure cooking was used for gelatinizing the triticale, commercial α-amylase SPEZYME ETHYL and glucoamyl- ase FERMENZYME L-400 were applied for starch liquefaction and saccharification. The process was conducted at 30°C for 72 h, next after distillation the stillage was centrifuged and the liquid fraction was used instead of 75% of process water. Results. Ethanol yield from triticale fermentations during 40 cycles ranged between 82% and 95% of theoretical yield preserving yeast vitality and quantity on the same level. The obtained distillates were characterized with enhanced volatile compounds (fusel oil, esters, aldehydes, methanol) as well as protein and potassium concentrations. Conclusions. The liquid part of stillage was proved that can be reused instead of water in bioethanol production from triticale, without disturbing the fermentation process. This investigated solution of distillery byproducts utilization (liquid phase of stillage) constitutes the way which could significantly decrease the bioethanol production costs by reducing the water consumption, as well as wastewater production.
PL
Wprowadzenie. Wywar gorzelniczy to podstawowy i bardzo uciążliwy produkt uboczny powstający w gorzelniach podczas produkcji etanolu. Celem prezentowanych badań była ocena możliwości redukcji powstawania wywaru oraz zużycia wody poprzez zawracanie płynnej części wywaru do ponownego użycia w procesie zacierania. Analizie poddano liczbę możliwych recyrkulacji bez negatywnego wpływu na wydajność procesu produkcji etanolu. Materiał i metody. Do procesów fermentacyjnych użyto pszenżyto ozime odmiany BOGO oraz preparat Ethanol Red drożdży gorzelniczych Saccharomyces cerevisiae. W doświadczeniu zastosowano metodę bezciśnieniowego uwalniania skrobi oraz enzymy: alfa-amylaze Spezyme Ethyl i glukoamylaze Fermenzyme w celu upłynnienia i scukrzenia zacierów. Proces prowadzono w temp. 30°C przez 72 h, następnie po oddestylowaniu alkoholu uzyskany wywar odwirowywano, a jego płynną część zawracano (tak że stanowiła 75% wody potrzebnej do przygotowania kolejnego zacieru). Wyniki. Wydajność produkcji etanolu z pszenżyta podczas 40 cykli recyrkulacji zacieru mieściła się od 82% do 95% wydajności teoretycznej bez wpływu na żywotność drożdży. Uzyskane destylaty charakteryzowały się zwiększoną zawartością związków lotnych (oleje fuzlowe, estry, aldehydy, metanol) oraz wyższą koncentracją białka i potasu. Wnioski. Stwierdzono, że istnieje możliwość kilkukrotnego wykorzystania płynnej części wywarów uzyskiwanych podczas produkcji etanolu z pszenżyta bez istotnych zakłóceń procesu. Prezentowane rozwiązanie utylizacji produktu ubocznego gorzelni (płynnej części wywaru) wskazuje na możliwość bardzo istotnego obniżenia kosztów produkcji bio- etanolu poprzez mniejsze zużycie wody oraz redukcję uciążliwych zanieczyszczeń.
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tom 63
EN
The poultry industry in Nigeria keeps expanding on a daily basis and it is faced with the problem of egg glut almost yearly. Most of the poultry are raised in rural environments where there is no available and avoidable power supply for egg preservation. The study was aimed at using locally available and cheap materials for preservation of eggs quality under the hot ambient temperature. The eggs were divided into three groups of 80 eggs each. The first and second groups were treated with vegetable oil (soybean oil) and shea butter, respectively, while the third group served as control. The eggs were stored under the ambient conditions and assessed for their physical and nutritional qualities on days 1, 8, 15, 22, 29, 36, 43 and 50. The physical quality was assessed by determining the albumen height and the Haugh’s unit, while the nutritional quality was appraised through the protein concentration of the albumen. Based on the values of Haugh’s unit, eggs treated with vegetable oil produced excellent results; good quality eggs were obtained up to 50th day of storage. Treatment with shea butter maintained quality eggs up to the 29th day, while with untreated eggs good physical quality was maintained up to 22 nd day of storage. Protein concentrations of eggs in the three groups studied decline with duration of storage, however the protein concentrations of eggs treated with vegetable oil were significantly higher than the other groups (p<0.001). The eggs treated with shea butter recorded a comparative higher protein concentrations than the untreated eggs (p=0.002).
EN
Oxidative metabolism is essential for the gamete and the embryo energy production and is unavoidably associated with generation of reactive oxygen species (ROS). Enzymatic antioxidant defenses are present in the mammalian oocytes, embryos and follicular fluid (FF). An addition of porcine FF to maturation media have positive effects on the IVM and IVF results. The aim of this study was to study the CAT, SOD and GSH-Px activity, as well as Cu, Mn, and Zn concentration in porcine FF collected from the left and the right ovary. The ovaries were collected from 77 gilts at age 8 months. All the analyzed samples of FF revealed active enzymes (24.2·10–3 U · l–1, 2.65·10–3 U · l–1, and 525 U · l–1 for CAT, GSH-Px, and SOD, respectively) and contained Zn, Cu and Mn (13.8·103mol · l–1, 33.3·103 mol · l–1, and 133·10–9 mol · l–1, respectively). In the pFF collected from the left ovary, SOD and GSH-Px activity was higher compared to pFF from the right ovary. On the contrary, the concentration of Cu and Mn was significantly lower in the left ovary pFF. The concentration of Cu ions was negatively correlated with SOD activity. The CAT activity in pFF form left or right ovary did not show any differences.
PL
Metabolizm oksydacyjny jest niezbędny do produkcji energii na potrzeby gamet i zarodków, co związane jest z wytwarzaniem reaktywnych form tlenu. Antyoksydacyjny mechanizm ochrony enzymatycznej znajduje się w oocytach i zarodkach ssaków, a także w płynie pęcherzykowym. Dodatek świńskiego płynu pęcherzykowego do odżywek hodowlanych korzystnie wpływa na wyniki zapłodnienia i dojrzewania in vitro. Celem badań było określenie aktywności katalazy (CAT) dysmutazy ponadtlenkowej (SOD) i persoksydazy glutationowej (GSH-Px) oraz koncentracji Cu,Mn i Zn wpłynie pęcherzykowym świń pobranym z prawego i lewego jajnika. Ogółem pobrano jajniki od 77 loch w wieku 8 miesięcy.Wewszystkich analizowanych próbkach badane enzymy wykazywały aktywność (24,2; 2,65 10–3 U · l–1 i 525 U · l–1 odpowiednio w przypadku CAT, GSH-Px i SOD) i zawierały jomy Zn, Cu i Mn (odpowiednio 13,8 · 103 mol · l–1; 33,3 · 103 mol · l–1 i 133 · 10–9 mol · l–1). W płynie pęcherzykowym pobranym z lewego jajnika aktywność SOD i GSH-Px była wyższa niż w płynie pęcherzykowym prawego jajnika. Natomiast koncentracja Cu i Mn była istotnie niższa w płynie z lewego jajnika. Koncentracja jonów Cu była skorelowana ujemnie z aktywnością SOD. Aktywność CAT w płynie pęcherzykowym z prawego lub lewego jajnika nie wykazała różnic.
EN
Leptin is a 16-kDa protein hormone encoded by the obese (ob) gene and acts on receptors in the hypothalamus to regulate food intake and energy balance. The identification of leptin and its receptor mRNAs and proteins in human and mouse endometrium and placental trophoblast has attracted attention to the potential role of leptin in implantation. Thus, the aim of this study was to compare the expression levels of porcine leptin mRNA and protein in endometrium and myometrium during mid- and late-luteal phases of the oestrous cycle (days 10 - 12 and 14 - 16) as well as during two stages of pregnancy respondent to the beginning (days 14 - 16) and the end (days 30 - 32) of the implantation process, and in trophoblast during both periods of pregnancy. Leptin gene and protein expression in myometrium, and leptin mRNA expression in endometrium was more pronounced in the mid- and late-luteal phases of the cycle in comparison to studied periods of pregnancy, whereas leptin protein concentration in endometrium was either enhanced on days 30 - 32 of pregnancy in relation to days 14 - 16 of the cycle or there were no changes between pregnancy and luteal phase of the cycle. On days 30 - 32 of pregnancy, expression of the leptin gene in the endometrium, and of the leptin gene and protein in the myometrium was more pronounced in comparison to the earlier stage of pregnancy. Moreover, leptin gene expression in porcine trophoblast increased during the beginning of the implantation process compared to days 30 - 32 of pregnancy, while the protein concentration decreased on days 14 - 16 of pregnancy. In conclusion, the finding of leptin gene and protein expression in porcine endometrium, myometrium and trophoblast indicates that locally synthesised leptin can participate in the control of pig reproduction. The fluctuation of the hormone concentration during pregnancy and changes in its level between pregnancy and the oestrous cycle may indicate leptin's involvement in the implantation process.
EN
The present study involved 180 slaughter turkey-hens of heavy Big-6 type divided into four groups (in triplicate repetition for 15 birds). All the birds were fed with the same standard full-dose mixtures in 5-stage system. The turkey-hens of groups I, II and III were given 1,2,4-triasole derivative (3-(2-pyridil)-4-phenyl-l,2,4-triasole-5-carboxylic acid), which has antibacterial, antifungal and immunomodulating properties, in amount of 50, 75 and 100 |ig per 1 dm3 of water. Group IV - control was given water without the additive. The 1,2,4-triasole derivative was given to drinking water, starting from the first day of bird's life and for the whole rearing period. The present results of biochemical analysis of blood plasma showed that addition of examined substance significantly reduced concentration of protein, glucose, triglycerides and uric acid as compared to control. It was stated that tested 1,2,4-triasole derivative elevated the level of HDL fraction percentage and alkaline phosphatase activity in blood plasma.
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