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Oxidant defence enzymes in different isolates of Trichinella

100%

Hadas E. , Rodriguez-Caabeiro F. , Gonzales A. J.

Acta Parasitologica

1994

tom 39

nr 1

The prevention of biological damage brought about by free radical mediated mechanisms can be achieved by specific chemical scavengers which trap free radicals as well as by protective enzymes that remove hydrogen peroxide or superoxide radicals. The aim of this study was to carry out a comparative analysis of enzymes acting against free-radicals and peroxides in Trichinella spiralis, T. nelsoni and T. pseudospiralis isolates. The activity of superoxide dismutase in T. pseudospiralis was about two times higher than in T. spiralis and about 1.5 times higher than that of T. nelsoni. In all the isolates only one band representing superoxide dismutase was identified on electrophoretic analysis. Catalase and peroxidase activities were very low. Using electrophoretical analysis we found three identical bands of peroxidase activity in the species investigated. Our data suggest that, with regard to the anti-oxidant enzymes, superoxide dismutase plays an important role whereas peroxidase and catalase play lesser roles in the defence of Trichinella spp. larvae against host generated free radicals released during the response to infection.

Responses of root growth and protective enzymes to copper stress in turfgrass

51%

Zhao S. , Liu Q. , Qi Y. , Duo L.

tom 52

nr 2