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1 Bulletin of the Veterinary Institute in Puławy

1 2009

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Effects of aflatoxin B1, ochratoxin A, patulin, citrinin, and zearalenone on the in vitro proliferation of pig blood lymphocytes

100%

Stec J. , Zmudzki J. , Rachubik J. , Szczotka M.

nr 1

129-134

The effects of aflatoxin B₁ (AFB₁), ochratoxin A (OTA), patulin (PAT), citrinin (CIT), and zearalenone (ZEA) on in vitro response of pig peripheral blood mononuclear cells to mitogen concanavalin A was assayed after three days of incubation using ³H- thymidine uptake. Dose response curves for each mycotoxin were generated and the concentrations producing 50% inhibition of cell proliferation (IC₅₀) were estimated. AFB₁, OTA, and PAT were the most potent toxins with the IC₅₀ of 0.06, 0.17, and 0.19 µmol/L, respectively (0.2, 0.7, and 0.3 µg/mL, respectively). Based on the molar concentration, the inhibition potencies relative to that of AFB₁ were determined. OTA had 35% and PAT 31% potency to that of AFB₁, but CIT and ZEA had only 1.6 and 1.9 of AFB₁ inhibition potencies.