The purpose of the study was to determine a proper withdrawal time in case of Mastisan PN/MC, a new drug designed for the treatment of mastitis in dairy cows. The examinations were carried out on six healthy cows and 20 animals with clinical signs of mastitis. Mastisan PN/MC was administered once into two quarters of the udder in normal cows and into one quarter of animals with mastitis. The findings were evaluated by the rapid diffusion test (STD) based on Bacillus stearothermophilus and Penzym test. The presence of drug residues was found between 12-60 hours after the administration of Mastisan PN/MC. Therefore, the withdrawal time was established at 72 hours after a single application of Mastisan PN/MC or the last intramammary one.
The studies were carried out on 228 cows with clinical symptoms of mastitis in one or more quarters. Mastisan PN/MC was administered only once into 318 sick quarters. The preparation was made out in the Veterinary Institute on the basis of the experiences of the author. One dose of Mastisan PN/MC contains 600 000 iu of procainic penicillin, 300 000 iu of neomycin and components intensifying phagocytosis. Control examinations including clinical, cellular (CMT) and bacteriological tests were performed on the day 7, 14, 21 and 28 after treatment. The full effect of therapy was established in 87.1% of treated quarters in 85.5% of cows. It was found that Mastisan PN/MC revealed a high therapeutic efficacy in mastitis caused by Streptococcus agalactiae, S. dysgalactiae, S. uberis. Staphylococcus aureus, Staph. epidermidis, Micrococcus sp. and Escherichia coli. The best effects were noted both in subacute and acute forms of mastitis.
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