Wyniki wyszukiwania - Biblioteka Nauki

1

Identification of acidic and basic forms of pathogenesis-related [PR] proteins in potato leaves infected with potato virus Y

100%

Marczewski W.

Phytopathologia Polonica

|

1994

|

tom 08

2

Biological and molecular characterization of two tomato strains of potato virus Y [PVY]

100%

Morel C. , Gognalons P. , Guilbaud L. , Caranta C. , Gebre-Selassie K. , Marchoux G. , Jacquemond M.

|

tom 22

|

nr 3

3

Resistance of wild Nicotiana species to different PVY isolates

86%

Doroszewska T. , Depta A.

Phytopathologia

|

2011

|

tom 59

4

Simple and rapid technique to detect PVY presence in some Solanaceae plants

86%

Krzymowska M. , Hennig J.

Acta Physiologiae Plantarum

|

1997

|

tom 19

|

nr 2

EN

The study of plant-virus interactions requires a method which enables not only the detection of virus presence, but also its distribution in the infected plant tissue. We adopted a tissue print immunoblot technique for localization of potato virus Y (PVY) coat protein (CP) in infected potato and tobacco tissue. In potato stem cross-sections PVY CP was detected on the whole surface of the prints, however significantly higher concentration was observed in epidermis and phloem tissue. In the petiole of infected tobacco leaves the presence of viral protein was confined mainly to peripheral layers of parenchyma and epidermis. In phloem tissue the signal was also visible but it was significantly weaker. In our hands this approach is highly specific and gives resolution on the level of individual cells, thus it can be applied in several fields. As potyviral capsid proteins are involved in several events of pathogenesis the technique for immunolocalization of PVY CP could provide information which will shed new light on the virology of PVY.

5

Differentiation of potato virus Y [PVY] isolates

86%

Chrzanowska M.

Phytopathologia Polonica

|

1994

|

tom 08

6

Potato and tobacco cultivars transformation towards potato virus Y resistance

86%

Chachulska A. M. , Chrzanowska M. , Flis B. , Krzymowska M. , Lipska-Dwuznik A. , Robaglia C. , Zagorski W.

Biotechnologia

|

1997

|

nr 4

7

Comparison between PVY isolates obtained from potato and tobacco plants grown in Poland

86%

Chrzanowska M. , Doroszewska T.

Phytopathologia Polonica

|

1997

|

tom 13

8

Ultrastructural studies Nicotiana clevelandii plants doubly infected with potato leafroll virus and potato virus Y

72%

Garbaczewska G. , Kerlan C.

Annals of Warsaw Agricultural University. Agriculture

|

2001

|

nr 39

17-26

9

Analiza porazenia wirusem Y elit ziemniaka ze zbioru 1987 roku jako proba oceny pracy nasiennej

72%

Gabriel W. , Wydzialkowska J. , Dulinska B. , Gorczynski J. , Woznica W. , Zgorski L.

Biuletyn Instytutu Ziemniaka

|

1992

|

nr 41

19-30

10

Management of potato virus Y (PVY–NTN) causing potato tuber necrotic ringspot disease (PTNRD) in potato by prior treatment with a mild PVY strain

72%

Abdalla O. A. , Eraky A. I. , Mohamed S. A. , Fahmy F. G.

Journal of Plant Protection Research

|

2018

|

tom 58

|

nr 2

EN

Potato virus Y (PVY) is one of the most destructive viruses infecting potato in Egypt and worldwide. Recent research has shown that a necrotic PVY-NTN strain is infecting potato in Upper Egypt. Chemical control is not effective to control this viral pathogen. An alternative to control PVY infecting potato is using a mild PVY strain to elicit systemic cross protection in potato plants against infection with a severe necrotic strain of PVY. Results of this study showed that a PVY necrotic strain produced a significant lesser number of local lesions on diagnostic plants (Robinia pseudoacacia L.) when these plants were treated first with a mild PVY strain. Data obtained from greenhouse and field experiments indicated that treatment of potato plants (variety Burna) with a mild PVY strain significantly protected potato from infection with a severe necrotic PVY strain, and resulted in a significant increase in tuber yield compared with infected plants without prior treatment with a mild PVY strain. The highest increase in potato tuber yield was obtained when potato plants were inoculated with a mild PVY strain 3 days before challenging with the severe necrotic PVY strain. This study proved that using a mild strain of PVY can significantly protect potato plants from infection with a severe strain of this virus under both greenhouse and field conditions and can present a potential method to reduce losses due to infection of this virus in Assiut governorate and Upper Egypt.

11

Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants

72%

Wojtal I. , Piontek P. , Grzela R. , Jarmolowski A. , Zagorski W. , Chroboczek J.

Acta Biochimica Polonica

|

2011

|

tom 58

|

nr 3

EN

Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants that contained the VPg gene with the correct sequence. They generated VPg mRNA, but VPg protein was not detected. Some plants were immune to PVY infection suggesting post-transcriptional gene silencing. However, the likely PVY VPg toxicity exerted at an early stage of transformed seeds development precludes its use for engineering pathogen-derived resistance.

12

Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants

72%

Yazdi S. E. , Mulabisana J. , Prinsloo G. , Cloete M. , Kritzinger Q.

Journal of Plant Protection Research

|

2018

|

tom 58

|

nr 4

EN

The tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals and pollution of the environment has led to investigations into new non-toxic and biological treatments to control plant viral diseases. Ethanolic extracts of Bowiea volubilis (bulbs), Cotyledon orbiculata (leaves), Gomphocarpus fruticosus (leaves), Merwilla plumbea (dry and fresh bulbs), Nerium oleander (leaves), and the fruits and leaves of Strophanthus speciosus, were evaluated against PVYNTN in vivo and in vitro. At a concentration of 20 mg · ml−1, ethanolic extracts of Strophanthus speciosus (leaves) and fruits (50 mg · ml−1) significantly reduced the expression of PVYNTN symptoms on tobacco plants in vitro without affecting the normal growth and development of the plant. Similarly, at 50 mg · ml−1, N. oleander, C. orbiculata and B. volubilis (fresh bulbs) and S. speciousus leaves at 20 mg · ml−1 extracts showed significant differences in PVYNTN symptoms in the in vivo experiment. Strophanthus speciosus leaf and fruit extracts showed significant inhibition in the in vitro and in vivo assays and demonstrated that S. speciosus has potential to be used as an antiphytoviral treatment.

13

Necrosis in Solanum tuberosum stems infected with potato virus Y by grafting

72%

felczak P. , Garbaczewska G. , Otulak K.

Acta Biologica Cracoviensia. Series Botanica

|

2010

|

tom 52

|

nr 1

61-66

EN

This work examined the distribution of necrosis on stems of two cultivars of potato (Ania, Glada) with different levels of resistance to PVY infection. Potato virus Y particles and/or cytoplasmic (CI) and amorphous inclusions (AI) were identified in insert and offshoot potato cells of susceptible cv. Glada. Cytoplasmic inclusions were not observed in insert and offshoot stems of resistant cv. Ania, although there were numerous deformations, degeneration and tissue necrosis. It was found that (1) necrotic reactions were the form of plant cell response for both the PVY-resistant and susceptible cultivars, (2) development of necrosis in vascular tissue did not prevent the pathogen from spreading outside the necrotic region in the less resistant cultivar (Glada), and (3) extreme resistance to PVY in potato plants, determined by the Rysto gene, was manifested in the absence of virus particles and cytoplasmic inclusions in infected plant cells.

14

Effect of mineral oil on Myzus persicae capability to spread of PVY and PVM to successive potato plants

72%

Wrobel S.

Journal of Plant Protection Research

|

2007

|

tom 47

|

nr 4

383-390

EN

The work involved assessment of the Myzus persicae (Sulz.) capability to infect successively potato plants with PVY and PVM after a Sunspray 850 EC mineral oil application. The tests were carried out in the greenhouse, with 4-week-old, healthy potato plants possessing low ressistance to viruses, derived from in vitro (test plants). Any time, for each combination and each virus, 10 successive plants were inoculated in 6 repetitions. Virus sources were potato plants infected with PVY or PVM, kept in isolated rooms. As a result of oil application, feeding of the M. persicae specimens on plants previously treated with this oil was delayed. The highest reduction as regards PVY and PVM transmission by M. persicae was obtained in the treatment where both plants constituting virus sources and test plants were protected, because only two of ten plants were infected with PVY, and only one with PVM. Mineral oil application only on potato test plants (healthy ones) reduced to a small degree M. persicae capability to transmit PVY to six successive plants (to seven in control), whereas it was much higher for PVM - to three (to six in control). In the case when only plants constituting virus sources were oil-protected, aphid's capability to transmit PVY was limited only to four plants, and PVM - to two. These results seem to confirm much more the hypothesis that mineral oil inactivates virus particles in the stylets of aphids while they attempt to acquire it from plants which have been previously protected with mineral oil.

PL

Oceniano zdolność mszyc Myzus persicae (Sulz.) do infekcji kolejnych roślin ziemniaka PVY i PVM, po zastosowaniu oleju mineralnego Sunspray 850 EC. Badania przeprowadzono w szklarni, wykorzystując jako materiał badawczy 4-tygodniowe, zdrowe rośliny ziemniaka o niskiej odporności na wirusy, pochodzące z in vitro (rośliny testowe). Dla każdej kombinaqi i każdego wirusa inokulowano każdorazowo 10 kolejnych roślin w 6 powtórzeniach. Źródła wirusów stanowiły rośliny ziemniaka porażone PVY lub PVM, które przetrzymywano w izolowanych kamerach. Zastosowanie oleju powodowało opóźnienie rozpoczęcia żerowania osobników mszyc M. persicae na roślinach wcześniej nim traktowanych. Największe ograniczenie przenoszenia PVY i PVM przez M. persicae uzyskano w kombinacji, gdy chroniono zarówno rośliny stanowiące źródło wirusa, jak i rośliny testowe, gdyż jedynie dwie z dziesięciu roślin uległy infekcji PVY i tylko jedna PVM. Zastosowanie oleju mineralnego jedynie na rośliny testowe ziemniaka (zdrowe) ograniczało w niewielkim stopniu zdolność mszyc do przenoszenia PVY do sześciu kolejnych roślin (na kontroli do siedmiu), natomiast znacznie bardziej w wypadku PVM - do trzech (na kontroli do sześciu). W sytuacji, gdy chroniono olejem jedynie rośliny stanowiące źródło wirusa, zdolność do przenoszenia PVY przez M. persicae ograniczona była do czterech roślin, a PVM do dwóch. Wyniki te zdają się potwierdzać bardziej hipotezę, że olej mineralny inaktywuje cząstki wirusa w mszycach w czasie próby nabycia go z roślin, które wcześniej były chronione olejem mineralnym.

15

Nutritional properties of tubers of conventionally bred and transgenic lines of potato resistant to necrotic strain of potato virus Y [PVYn]

72%

Juskiewicz J. , Zdunczyk Z. , Fornal J.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 3

EN

The potential effect of genetic modification on nutritional properties of potatoes transformed to improve resistance to a necrotic strain of Potato virus Y was determined in a rat experiment. Autoclaved tubers from four transgenic lines were included to a diet in the amount of 40% and compared with the conventional cv. Irga. The experiment lasted 3 weeks and special attention was paid to nutritional properties of diets, caecal metabolism and serum indices. Genetic modification of potato had no negative effect on the chemical composition and nutritional properties of tubers, ecosystem of the caecum, activity of serum enzymes and non-specific defence mechanism of the rats. Obtained results indicate that transgenic potato with improved resistance to PVYN: line R1F (truncated gene coding for PVYN polymerase in sense orientation), R2P (truncated gene coding for PVYN polymerase in antisense orientation), and NTR1.16 (non-translated regions of PVYN genome in sense orientation) are substantial and nutritional equivalence to the non-transgenic cultivar. Tubers of transgenic line NTR2.27 (non-translated regions of PVYN genome in antisense orientation) increased the bulk of caecal digesta and the production of SCFA as compared to tubers of the conventional cultivar and the other transgenic clones. Taking into account some deviations, it seems reasonable to undertake a long-term feeding study to confirm the nutritional properties of tubers of transgenic lines.

16

Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid [PSTVd], potato virus Y [PVY], and potato leafroll virus /PLRV/

72%

Welnicki M. , Zekanowski C. , Zagorski W.

|

nr 4

XX

A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUCl8 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT probe. This makes p3POT probe an useful tool for the routine testing, and selection of virus-free potatoes

17

Efekt roznych sposobow niszczenia naci w ograniczaniu porazenia sadzeniakow ziemniaka wirusem Y

58%

Wrobel S.

Progress in Plant Protection

|

2001

|

tom 41

|

nr 2

683-687

18

Prognoza porazenia sadzeniakow ziemniaka zbioru 1995 roku wirusami Y i lisciozwoju

58%

Kostiw M. , Woznica W. , Dulinska B. , Robak B.

Ziemniak Polski

|

1995

|

nr 3

40-43

19

Szerzenie sie szczepow PVY w Polsce

58%

Kaczmarek U. , Hnat A.

Progress in Plant Protection

|

1998

|

tom 38

|

nr 2

521-523

20

Prognoza porazenia sadzeniakow ziemniaka zbioru 1998 r. wirusami Y i lisciozwoju

58%

Kostiw M. , Robak B. , Dulinska B.

Ziemniak Polski

|

1998

|

nr 3

11-13