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1

Expression of cyclooxygenase-2 in the inflammatory changed porcine uterus

100%

Jana B. , Kozlowska A. , Koszykowska M. , Majewski M.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 1

EN

In the present study, the pattern of cyclooxygenase-2 (COX-2) expression in health and inflamed porcine uteri was analyzed using real-time reverse transcriptase-polymerase chain reaction (RT-PCR),Western blot and immunohistochemistry. On day 3 of the estrous cycle, 50 ml of saline or 50 ml of Escherichia coli (E. coli) suspension containing 10⁹ colony-forming units/ml, were injected into each uterine horn of the control (n=6) and experimental gilts (n=7), respectively. This latter procedure lead to a moderately (n=3) or severely intense (n=4) acute endometritis after eight days. Expression of both the COX-2 mRNA and protein was increased in the endometrium (ENDO) of animals suffering from the moderate (P<0.05, P<0.01, respectively) and severe (P<0.01) acute endometritis, as compared to the control tissues. Moreover, COX-2 mRNA level and protein content were higher (P<0.05) in the ENDO of animals with severe than with a moderately acute endometritis. An elevation in the COX-2 gene (P<0.05) and protein (P<0.001) expression was also observed in the myometrium (MYO) of animals suffering from severe endometritis, when compared with the levels observed in MYO of both the health and moderate intensely inflamed uteri. However, both the COX-2 mRNA and protein levels were similar in MYO of the control and moderately inflamed organs. The luminal epithelium, some of uterine glands and circular layer of the MYO were more intensely stained for COX-2 in animals with severe endometritis, than in animals with healthy or moderately inflamed uteri. Nonetheless, stronger COX-2 reaction was found in some of the uterine glands in latter group, when compared to that observed in uteri of the control animals. While positive COX-2-labeling was observed in the muscular layer of all arteries supplying the health and inflamed uteri, such staining was exclusively present in the endothelium of some arteries in inflamed organs. Likewise, some arteries in uteri of the animals with severe endometritis displayed immunoreaction stronger than that found in uteri of the animals with moderate inflammation. The present study revealed an up-regulation of COX-2 mRNA and protein in the inflamed porcine uterus, which was directly related to the intensity of the organ inflammation. An increase in the COX-2 expression in the uterus challenged by E. coli-induced inflammation indicates that this enzyme is crucial for elevated prostaglandins production in the inflamed organ.

2

The influence of inflammatory process on prostaglandin F2alpha contractile activity in porcine uterus

100%

Kucharski J. , Jaroszewski J. , Jana B. , Gorska J. , Kozlowska A. , Markiewicz W.

Journal of Animal and Feed Sciences

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2007

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tom 16

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nr 4

654-667

3

Immunoreactivity of iNOS in porcine uterus after infusions of Escherichia coli endotoxin

100%

Jana B. , Andronowska A. , Kucharski J.

Folia Histochemica et Cytobiologica

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2001

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tom 39

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nr 2

4

Nitric oxide mediates an inflammatory effect of Escherichia coli in the porcine uterus

100%

Jana B. , Andronowska A. , Kucharski J.

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nr 4

EN

The purpose of this study was to determine whether inflammatory changes in uterus caused by Escherichia coli are associated with changes in nitric oxide (NO) production. Therefore, the activity of NADPH-diaphorase (NADPH-d), a histochemical marker for nitric oxide synthase (NOS), localization of inducible isoform of NOS (iNOS) and tissue content of nitrite were studied in the uterine structures after inoculation of Escherichia coli into the uterus in gilts. Twelve sexually matured gilts with controlled estrous cycle were used. The animals were laparotomized on the 2nd day of the estrous cycle and polyvinyl cannulas were inserted into the uterine horns to infuse saline or Escherichia coli. In the group I (control; n=6), 25 ml of saline was infused into each uterine horn on the 4,h day of the estrous cycle. At the same time, 25 ml of Escherichia coli (strain 025:K23/α/:Hl) suspension, containing 107 colony forming units/ml was inoculated into each uterine horn of the treated gilts (group II; n=6). The middle part of the uterine horn was collected on the 14th day of the next estrous cycle immediately after slaughter. Cryostat sections from paraformaldehyde fixed tissues were stained histochemically to study the activity of NADPH-d and immunohistochemi- cally to investigate the distribution of iNOS. Optical density was assessed to evaluate the intensity of the histochemical reaction. Nitrite content was measured spectrophotometrically. In the Escherichia coli-treated gilts, the activitý of NADPH-d in the luminal epithelium and in external parts of excretory ducts of uterine glands was higher (P < 0.001) as compared to that in the control animals. In the secretory part of the uterine glands the activity of this enzyme was similar in both groups of the gilts. In the gilts that received Escherichia coli, the histochemical reaction of NADPH-d in endometrial blood vessels was stronger than that found in the control animals. Immunoreactivity for iNOS in the luminal epithelium, in external parts of excretory ducts of uterine glands and in vascular endothelial cells was stronger in the Escherichia coli-treated gilts as compared to that observed in the control animals. Only weak or no immunoreactivity was found in the secretory part of the uterine glands in both groups of the gilts. After Escherichia coli inoculation, nitrite content in the uterine tissues was higher (P < 0.05) than that determined in the controls. Our study has revealed that infusion of Escherichia coli into the porcine uterus induces the activity of NADPH-d, iNOS and increases the tissue content of nitrite in this organ. The data obtained indicate that NO can mediate an inflammatory effect of Escherichia coli in the uterus.

5

Novel aspects of cytokine action in porcine uterus - endometrial and myometrial production of estrone (E1) in the presence of interleukin 1beta (IL1beta), interleukin 6 (IL6) and tumor necrosis factor (TNFalpha) – in vitro study

84%

Franczak A. , Wojciechowicz B. , Kotwica G.

Folia Biologica

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2013

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tom 61

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nr 3-4

6

Escherichia coli-induced inflammation changes the expression of acetylcholine receptors (M2R, M3R, and α-7 nAChR) in the pig uterus

84%

Jana B. , Galka J. , Palus K. , Sikora M.

Journal of Veterinary Research

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2020

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tom 64

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nr 4

7

Absorption of proinflammatory cytokines from inflamed porcine uterus into the uterine venous blood - preliminary data

67%

Kucharski J. , Stefanczyk-Krzymowska S. , Jana B.

Polish Journal of Veterinary Sciences

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2008

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tom 11

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nr 1

9-16

EN

The aim of the present study was to estimate the absorption of 125I-labeled proinflammatory cytokines - interleukin-lß (IL-lß), interleukin-6 (IL-6) and tumor necrosis factor-a (TNF-a) from inflamed porcine uterus into the uterine venous blood. Moreover, in order to test the hypothesis that the above cytokines penetrate directly into ovaries and oviduct via local destination transfer in the area of the ovarian vascular pedicle and bypassing the systemic circulation, the concentration of IL-lß, IL-6 and TNF-a in ovarian and oviductal tissues was also studied. These cytokine concentrations were also estimated in the ovarian venous blood. IL-lß, IL-6 and TNF-a from both control and inflamed uteri were absorbed into the uterine venous blood, but it was higher (P < 0.05-0.001) from the pathologically changed uteri. The uterine tissues, particularly the endometrium, of both control and inflamed uteri retained all studied cytokines, but to a higher degree (P < 0.001) in the inflamed uteri. Injections of IL-lß, IL-6 and TNF-a into the control and inflammatory changed uteri produced the presence of these proteins in the ovary and oviduct. However, the concentrations of IL-lß and IL-6 in the ovarian and oviductal tissues was low after injections of control and inflamed uteri with these cytokines. In turn, administration of TNF-a into the inflammatory changed uteri lead to an enhancement in the concentration of this cytokine in the ovarian parenchyma (P < 0.05) and oviduct (P < 0.001). All studied cytokines were found in the ovarian venous blood after their injection into both control and inflamed uteri, which indicated its local destination transfer to the ovary. However, the concentration of cytokines increased (P < 0.05-0.001) in the gilts with pathologically changed uteri as compared to controls. The study showed that both control and inflamed porcine uteri absorbed IL-lß, IL-6 and TNF-a into the uterine venous blood, but the values of absorbed cytokines from inflamed uteri were higher. Moreover, the quantity and the manner of the studied cytokines absorption into the uterine venous blood differed.

8

Interleukin 1beta-induced synthesis and secretion of prostaglandin E2 in the porcine uterus during various periods of pregnancy and the estrous cycle

67%

Franczak A. , Zmijewska A. , Kurowicka B. , Wojciechowicz B. , Kotwica G.

Journal of Physiology and Pharmacology

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2010

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tom 61

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nr 6

9

Expression and cellular distribution of NADPH-diaphorase and nitric oxide synthases in the porcine uterus during early pregnancy

67%

Andronowska A. , Chrusciel M.

Folia Histochemica et Cytobiologica

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2007

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tom 45

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nr 4

375-380

10

Relative transcript abundance of oxytocin receptor gene in porcine uterus during luteolysis and early pregnancy

67%

Oponowicz A. , Franczak A. , Kurowicka B. , Kotwica G.

Journal of Applied Genetics

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2006

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tom 47

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nr 4

EN

The aim of the present study was to investigate transcript localization of the oxytocin receptor (OTR) gene in different cells of the porcine uterus during luteolysis and early pregnancy (days 14-16) using in situ hybridization (ISH). OTR mRNA was localized in the uterine luminal epithelium (LEC), glandular epithelium (GEC), stromal cells (SC) of the endometrium, in the longitudinal muscle layer (LM) and circular muscle layer (CM) of the myometrium. The OTR transcript was quantified by optical density units of silver grains. The OTR transcript levels in the endometrium and myometrium were statistically higher during luteolysis than during early pregnancy (P < 0.05). Besides, during luteolysis, the mRNA level was higher in the LEC, GEC of the endometrium and LM of the myometrium compared to that observed in the SC of endometrium and CM of the myometrium, respectively (P < 0.05). In summary: 1) the level of OTR mRNA in uterine tissues is higher during luteolysis compared to early pregnancy, 2) the OTR transcript level in endometrial cells did not correspond to the sensitivity of these cells to oxytocin (ОТ), 3) the myometrial expression of the OTR gene is appropriate to control contractile activity and secretion of PG during luteolysis.

11

The effect of oxytocin on hCG action in phosphoinositide turnover in porcine myometrial smooth muscle cells

59%

Kisielewska J. , Ziecik A. J.

Journal of Physiology and Pharmacology

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1995

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tom 46

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nr 3