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1
Content available remote Qualitative and quantitative analysis of polyphenolic compounds in Ilex Sp.
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EN
Natural compounds are an important source of desired biological activity which help to improve nutritional status, enhance productivity and bring many health benefits. The leaves of the Ilex paraguariensis (Aquifoliaceae) are used for preparing a beverage known as yerba mate and represent a proven source of natural polyphenols which are known to foster biological activity with the emphasis on antioxidant properties. In present work we focused on the polyphenolic content of air-dried leaves of Ilex aquifolium L., Ilex aquifolium ‘Argentea Mariginata’, Ilex meserveae ‘Blue Angel’, and a commercially available mate as the reference product. Liquid chromatography combined with mass spectrometry (HPLC and LC-MS) and thin layer chromatography (TLC), were used to establish polyphenolic substances content in aqueous methanolic extracts obtained from the biological matter. Up to 20 polyphenolic compounds were identified in the extracts, including rutin, quinic acid and its caffeoyl esters, i.e. chlorogenic acid and its isomers as well as dicaffeoyl derivatives. We took chlorogenic acid and rutin as reference compounds to quantify their levels in the extracts. It was determined that in all tested plants, high levels of these antioxidants were present. This led us to the conclusion that their leaves might serve as valuable food additives.
EN
This study investigates the influence of extraction system on the extractability of polyphenol compounds and antioxidant activity of various medicinal plants. Oxygen radical absorbance capacity (ORAC) and total polyphenol content of 25 Bulgarian medicinal plants subjected to water or 80 % acetone extractions were investigated and compared. The type of extragent significantly influenced the efficiency of the polyphenol extraction and the antioxidant activity. In all cases ORAC results and total polyphenol content were higher for acetone extraction than for water extraction. The acetone extract of peppermint had the highest ORAC value - 2917 µmol Trolox equivalent (TE)/g dry weight (DW) and polyphenol content - 20216 mg/100 g DW. For water extraction thyme exhibited the highest ORAC antioxidant activity - 1434 µmol TE/g DW. There was a significant linear correlation between the concentration of total polyphenols and ORAC in the investigated medicinal plants. It can be concluded that the solvent used affects significantly the polyphenol content and the antioxidant activity of the extract and therefore it is recommended to use more than one extraction system for better assessment of the antioxidant activity of natural products. Several of the investigated herbs contain substantial amounts of free radical scavengers and can serve as a potential source of natural antioxidants for medicinal and commercial uses.
EN
Strong radical-scavenging activity of Geranium macrorrhizum extracts isolated by using various solvent systems has been reported previously. This study aimed at expanding the knowledge on the bioactivities of antioxidatively active G. macrorrhizum butanol fraction, which was isolated from ethanolic extract (EB), and water fraction, which was isolated from water extract (WW) by measuring their singlet oxygen scavenging properties, as well as preliminary assessment of cytotoxicity and genotoxicity toward mammalian cells. The cytotoxicity (necrosis induction) of the extracts in bovine leukemia virus-transformed lamb kidney fibroblasts (line FLK) was partly prevented by antioxidants and stimulated by the prooxidant BCNU (N,N'-bis(2-chloroethyl)-N-nitrosourea). This indicates that the cytotoxicity of G. macrorrhizum extracts is at least partly attributed to their prooxidant action, presumably due to the formation of quinoidal products of their (auto)oxidation. The latter was evidenced by the nature of the peroxidase-catalyzed oxidation products, which supported DT-diaphorase-catalyzed oxidation of NADPH and participated in conjugation reactions with reduced glutathione. The genotoxic properties were studied using chromosome aberration (CA) and sister chromatid exchange (SCE) tests in human lymphocytes in vitro and Drosophila melanogaster somatic mutation and recombination test (SMART) in vivo. In the CA test, only the highest doses of both fractions significantly increased chromosome aberration frequency. In the SCE test, both fractions induced SCEs in a clear dose-dependent manner. G. macrorrhizum extracts were not genotoxic in the SMART test in vivo. Our data indicate that in spite of the possible beneficial (antioxidant) effects of Geranium extracts, the possibilities of their use as ingredients of functional foods and/or food supplements should be further examined due to their cyto- and genotoxic effects resulting mainly from the action of quercetin-derived components abundant in the extracts.
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EN
Tea, Camellia sinensis L. is the most widely drunk beverage in the world. Comprehensive healing properties of this refreshing, mildly stimulating drink are known in traditional medicine dating back almost 5000 years. Tea healing and disease prevention mechanism is mostly based on antioxidant properties. Tea beverage is a rich source of polyphenols, especially catechins which are very powerful antioxidants. The most abundant catechins in tea beverage are epigallocatechin 3-gallate (EGCG), epigallocatechin (EGC), epicatechin 3-gallate (ECG), and epicatechin (EC). Many investigations i.e. population-based, screening, involving patients and also with animal or cell models, show many valuable pharmacological properties of tea. It is proved by numerous studies that drinking tea or taking tea extracts can prevent chronic diseases, including coronary heart disease and many types of cancer. It can also help with diabetes, hypertension and obesity and may contribute to inflammation reduce. Better understanding of tea healing properties could help reduce high costs of medical care, improve treatment of lifestyle diseases, and transform tea beverage into an evidence based functional food.
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Content available HPLC-DAD-ESI/MS Analysis of Arnica TM Constituents
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EN
The herbal drugs obtained from Arnica montana flowers are known for their anti-inflammatory activity. However, there is no detailed data on chemical constituents of the whole Arnica plant. HPLC-DAD-ESI/MS analysis of the whole Arnica montana plant (Arnica TM), used in the preparation of allopathic and homeopathic medicinal products, has been conducted. The qualitative and quantitative analysis comprised phenolic acids, flavonoids and sesquiterpene lactones – helenalin and dihydrohelenaline esters identified earlier in Arnica flowers. The analyzed material showed to be a rich source of caffeoylquinic acid (CQA) derivatives, especially 3,5-di-O-caffeoylquinic acid. According to the literature data, anti-inflammatory activity of extracts from the whole Arnica montana plant can be due to CQA presence as dominating compounds.
EN
Extrusion-cooking of plant materials may enhance antioxidant activity and improve health benefits. Selected antioxidant polyphenols in extruded corn gruels enriched with different amounts of linden flowers were determined by LC-ESI-MS/MS and quality characteristics were determined. Phenolic content increased with Tiliae inflorescentia addition and was not decreased by high-temperature extrusion. Linden flower incorporation into instant gruels should be limited to 10% to retain acceptable sensory properties.
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Fetal ductal constriction is a potentially severe functional alteration, often causing right ventricular overload and insufficiency, tricuspid regurgitation and neonatal pulmonary hypertension. Classically, maternal administration of indomethacin and/or other nonsteroidal antiinflammatory drugs interfere in prostaglandin metabolism, leading to ductal constriction. However, many cases of fetal ductal constriction, as well as of persistent neonatal pulmonary artery hypertension, remain without an established etiology, being referred as “idiopathic”. In recent years, a growing body of evidences has shown that herbs, fruits, nuts, and a wide diversity of substances commonly used in daily diet, because of their high content of polyphenols, have definitive effects upon the metabolic pathway of inflammation, with consequent inhibition of prostaglandins synthesis. This anti-inflammatory action of polyphenols, when ingested during the third trimester of pregnancy, may interfere with the dynamics of fetal ductus arteriosus flow and cause ductal constriction. This review has the purpose to approach these new evidences, which may influence dietary orientation during pregnancy.
EN
The three aims of the study were to: 1) evaluate the content of selenium and polyphenols, and to assess the reductive potential (FRAP test) and ability to deactivate ABTS•+ cation radicals in C. officinalis, H. perforatum, M. x piperita and S. marianum from Poland and Ukraine; 2) define the diversification in concentrations of selenium and polyphenols and in levels of ABTS activity and FRAP reductive potential within the examined species in relation to their location; and 3) evaluate the inter-dependencies between selenium, total polyphenol concentration, ABTS and FRAP activity, in the examined plants. We observed differences in the content of total polyphenols and antioxidative activity between the herb species and the location. Most selenium was found in M. x piperita and S. marianum from western Ukraine, with Polish H. perforatum from Pomerania and M. x piperita from Podlasie representing the highest TP content and antioxidative potential. A clear and significant relationship between the content of Se and antioxidative activity (ABTS and FRAP), and also between total polyphenols and antioxidative activity was noted only in H. perforatum. In C. officinalis a significant relationship was noted between Se and both total polyphenols and antioxidative potential. In M. x piperita a significant dependency was found between total polyphenols and antioxidative potential. The results of our research indicate a different role of selenium as an antioxidant in H. perforatum and C. officinalis, as well as polyphenols in H. perforatum and M. x piperita in dependence on the harvesting regions.
EN
In the present work, fruits from two plant species, Rosa canina L. and Sorbus aucuparia L., popular in traditional folk medicine in Serbia, were studied. The aim was to examine and compare the efficiency of the ultrasonic extraction with different solvents regarding physicochemical properties, polyphenolic profile of extracts, as well as their tyrosinase inhibitory and antioxidant activity. The polyphenols evaluation indicated that water was the best solvent for a thorough extraction of bioactive compounds from the R. canina fruits, while propylene glycol-water (45:55, v/v) was the most efficient regarding S. aucuparia fruits, followed by ethanol-water (7:3, v/v). Only flavonoids were more abundant in S. aucuparia fruit extracts. R. canina water extracts showed a higher antioxidant activity, using several in vitro tests with different working principles. However, S. aucuparia ultrasonic extracts with propylene glycol-water (45:55, v/v) demonstrated a higher potential concerning tyrosinase inhibitory and chelating activity. Therefore, these ultrasonic extracts, being great sources of natural anti-tyrosinase inhibitors and antioxidants, can be considered as promising candidates suitable for pharmaceutical application, as great sources of natural anti-tyrosinase inhibitors and antioxidants.
EN
Artemisia pallens L. (Compositae) is used in Indian traditional medicine to treat diabetes mellitus, jaundice, hysteria, body pain, and bacterial and fungal infections. A major cause of a variety of diseases is oxidative stress which is reduced by antioxidants such as polyphenols. These secondary metabolites are generally ubiquitous in plants and extensively used in the pharmaceutical, cosmetic, and food industries. In this study a simple and sensitive HPLC-UV-MS-MS-based method was developed for separation, identification, and quantification of polyphenols, for example gallic, protocatechuic, chlorogenic, caffeic, and ferulic acids, rutin, quercetin, and kaempferol. Amounts of polyphenols detected in 50% methanol-water extracts of the plant varied from 0.005% (kaempferol) to 0.24% (protocatechuic acid). Separation of the polyphenols was achieved on a reversed-phase C 18 with a mobile phase prepared from 1% aqueous with acetic acid and acetonitrile at a flow rate of 0.6 mL min -1 . The phenolic compounds were detected by UV absorption at 254 nm. The method was validated for linearity, accuracy, precision, LOD, LOQ, specificity, selectivity, and compound stability. Results from intra and inter-day validation (n = 6) showed the method was efficient and rapid. The optimized method was applied to extracts of A. pallens for identification and quantification of the polyphenols. The reference standards and their presence in A. pallens were confirmed by mass spectrometry.
EN
Extrusion cooking technology was applied for obtaining corn extrudates fortified with various level (10-20%) of rosehip pomace powder or apple pomace powder. The total polyphenols content, antioxidant activities (ABTS), organoleptic properties and colour of the extrudates were determined. Pomace addition increased the level of total polyphenols content and antioxidant activity in obtained corn - pomace extrudates, especially in samples enriched with rosehip pomace. Extrudates with 20% of rosehip pomace addition characterized the highest polyphenols content and antioxidant activity. Only the slight decrease of some quality features - shape and size, taste and fl avour, structure and colour of obtained extrudates was observed. Sample with fruit pomace addition showed increasing consistency evaluation. The extruded products by utilising fruit by-products got good evaluation of panelists and can be an excellent source of bioactive compounds in the daily human diet.
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Content available remote Charakterystyka związków fenolowych zawartych w miodach
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PL
Działanie lecznicze miodu wiązano przede wszystkim z obecnością w nim nadtlenku wodoru, będącego ubocznym produktem reakcji enzymatycznych, zachodzących w miodzie. W ostatnich latach wykazano, iż lecznicze właściwości miodów związane są też z działaniem antyoksydacyjnym niektórych składników miodów. Flawonoidy i kwasy fenolowe zawarte w miodach wykazują działanie bakteriobójcze, przeciwzapalne, przeciwalergiczne, przeciwzakrzepowe, przeciwnowotworowe. Zawartość poszczególnych fenolokwasów i flawonoidów zależy od źródła roślinnego. Celem niniejszego opracowania była próba zebrania informacji na temat tzw. profili fenolowych poszczególnych odmian miodów pszczelich oraz powiązania tych profili z działaniem leczniczym miodów.
EN
The therapeutic activity of honey has been associated with the presence of hydrogen peroxide, which is by-product enzymatic reactions occurred in honey. Recently the therapeutic activity have been connected with the antioxidants content of honey. Flavonoids and phenolic acids contained in honey shown antibacterial, anti-coagulantic, anti-allergic, anti-carcinogenic properties. The content of particular polyphenols depend on botanical origin. The aim of this study was to evaluate the polyphenols profiles of different kind of honey and clarify if it has connection with therapeutic activities of those honeys.
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Content available remote Effect of natural phenols on the catalytic activity of cytochrome P450 2E1
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EN
The effect of protocatechuic acid, tannic acid and trans-resveratrol on the activity of p-nitrophenol hydroxylase (PNPH), an enzymatic marker of CYP2E1, was examined in liver microsomes from acetone induced mice. trans-Resveratrol was found to be the most potent inhibitor (IC50 = 18.5 ± 0.4 mM) of PNPH, while protocatechuic acid had no effect on the enzyme activity. Tannic acid with IC50 = 29.6 ± 3.3 mM showed mixed- and trans-resveratrol competitive inhibition kinetics (Ki = 1 mM and 2.1 mM, respectively). Moreover, trans-resveratrol produced a NADPH-dependent loss of PNPH activity, suggesting mechanism-based CYP2E1 inactivation. These results indicate that trans-resveratrol and tannic acid may modulate cytochrome P450 2E1 and influence the metabolic activation of xenobiotics mediated by this P450 isoform.
EN
Type 2 diabetes mellitus (T2D) is a chronic diet-related disease which due to many dangerous complications has become a prominent health problem of the world. The aim of the study was to explore the in vitro activity of Japanese quince (Chaenomeles japonica L., family Rosaceae, JQ) fruit polyphenolic extract as modulator of carbohydrates metabolism. The research was designed to investigate the effect of JQ polyphenolic extract on glucose metabolism in human hepatoma HepG2 cell line cultured under normal non-metabolically changed and hyperglycemic conditions. Pretreatment of the cells with JQ preparation caused decrease of intracellular ROS generation and influenced mitochondrial membrane polarization which seemed to lead to AMPK activation. Further effects observed in HepG2 cells were associated with activation of the enzyme: elevation of glucose uptake and glycogen content, and alleviation of gluconeogenesis through modulation of PEPCK, PTP1B, FOXO1 and GLUT2/4 expression. These findings suggest that JQ polyphenols exhibit hypoglycemic effects via modulation of AMPK signaling in hepatocytes.
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Content available remote Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts
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EN
The present study provides a comprehensive data on the antioxidant, antimicrobial and neutrophil-modulating activities of extracts from six medicinal plants - blackberry (Rubus fruticosus) leaves, chokeberry (Aronia melanocarpa) leaves, hawthorn (Crataegus monogyna) leaves, lady's mantle (Alchemilla glabra) aerial parts, meadowsweet (Filipendula ulmaria) aerial parts and raspberry (Rubus idaeus) leaves. In order to analyze the antioxidant activity of the herbs, several methods (ORAC, TRAP, HORAC and inhibition of lipid peroxidation) were used. Blackberry leaves and meadowsweet extracts revealed the highest antioxidant activities via all methods. All extracts studied blocked almost completely the opsonized zymosan particle-activated ROS production by neutrophils from human whole blood. On the other hand, the effect of extracts on phorbol myristate acetate-activated ROS production was much milder and even nonsignificant in the case of chokeberry leaves. This latter result suggests that extracts (apart from their antioxidative activity) interfere with the signaling cascade of phagocyte activation upstream of the protein kinase C activation. The antimicrobial activity of the investigated extracts against 11 human pathogens was investigated using three different methods. Meadowsweet and blackberry leaves extracts had the highest antimicrobial effect and the lowest minimal inhibiting concentrations (MICs) against the microorganisms tested.
EN
The consumption of highbush blueberries has been growing rapidly in recent years due to their taste and health-promoting qualities. Various solutions have been sought to obtain the highest quality fruit after harvest. In the era of eco-friendly products, it is important that the methods used are natural and ecological. For this purpose, chitosan (CH) was sprayed five times on highbush blueberry bushes before harvesting. Different molecular weights of CH (5, 12, 21, 50, 125, and 500 kDa) were used in this study. The physical and biochemical characteristics of the fruit were investigated. The antioxidant activity, microbial contaminants, and mycotoxins in fruit were also analysed. Application of CH affected the quality of highbush blueberries after harvest. The molecular weight of CH had a significant effect on the studied traits. The application of high-molecular-weight CH improved physical characteristics such as the average weight of 100 blueberries, firmness, and puncture. Furthermore, the blueberries had a more intense blue colour; were characterised by a higher content of l-ascorbic acid and polyphenols, especially anthocyanins; and did not contain mycotoxins. Spraying with CH can be recommended in the organic cultivation of highbush blueberries to obtain robust fruit with health-promoting qualities.
EN
Chitosan could be an alternative to synthetic plant protection chemicals commonly used in strawberry cultivation. The aim of this study was to determine the effect of chitosan with different molecular weights on the yield, health, and quality of Korona cultivar strawberries. The largest number of strawberries was harvested from plants sprayed with chitosan with a molecular weight of 50k kDa (611 g/plant). On the other hand, the largest (1017 g/100 fruit) and the firmest (225 G mm) strawberries were harvested from plants sprayed with chitosan125k kDa. Compared with the control, chitosan with a molecular weight >50 kDa also increased the contents of l-ascorbic acid and polyphenols and the antioxidant activity and improved fruit colour compared with the control. The plants were also more resistant to grey mould infestation. The experiment has shown that how chitosan interacts with fruit depends on its molecular weight, and chitosan with a higher molecular weight has a better effect on most traits determining fruit quality.
PL
Herbata (Camelia sinensis) jest jednym z najczęściej spożywanych napojów na świecie. W artykule przedstawiono rodzaje herbaty, scharakteryzowano ich skład chemiczny oraz czynniki wpływające na zawartość poszczególnych związków. Napar z liści herbaty stanowi także bardzo ważne źródło związków przeciwutleniających i przeciwrodnikowych, istotne z punktu widzenia konsumenta oraz producenta żywności. Zaprezentowano także nowe kierunki zastosowania ekstraktów z liści herbaty jako przeciwutleniaczy w produktach zawierających tłuszcze.
EN
Tea (Camelia sinensis) is one of the most consumed beverages in the world. Types of tea, chemical composition and factors influencing content of selected substances are presented in the paper. Tea leave infusions are also important sources of substances possessing high antioxidant and antiradical potential, essential for the consumer's health and food producers. According to the wide spectrum of action, also new directions of tea leaves extracts use in lipid containing systems are presented.
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