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EN
The stigma of Ornithogalum sigmoideum is of dry and papillate type. The papillae are covered by a cuticle-pellicle layer, as revealed by staining. The activity of nonspecific esterase, acid phosphatase and peroxidase increases in the pellicle during the receptivity period. The style of O. sigmoideum is of the hollow type. Ultrastructural study of the cells lining the canal indicated that they are secretory cells and contain abundant endoplasmic reticulum, dictyosomes, mitochondria, plastids and ribosomes. After anthesis these organelles show degeneration at the end of the secretory phase. In canal cells, cytochemical tests showed the presence of acidic polyanions, insoluble and acidic polysaccharides, proteins and lipids. Before anthesis the canal cells are rich in polysaccharides, proteins and lipids. At maturity the cuticle is ruptured and secretory materials from the canal cells are released into the canal. In the unpollinated style of O. sigmoideum the exudates accumulated in the center of the canal; in pollinated pistils the same secretion materials were dispersed through the canal, which became wider.
EN
The aim of the present study was to find the source of postpollination increase in calcium content in the transmitting tract in the style of Petunia hybrida Hort. Cytochemical and radiographic studies revealed a progressive increase in the level of free and loosely bound calcium ions in the course of successive developmental stages of the pollen tubes in the pistil. A surprising phenomenon was the increase in Ca2+ content in the extracellular regions of the transmitting tract preceding the penetration of the pollen tubes therein. We suggest that the main sources of postpollination increase in calcium in the pistil are the walls of the transmitting cells and the intercellular matrix and, probably, additional Ca2+ uptake, initiated by pollination.
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