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EN
Gypsophila aretioides, a cushion form evergreen plant, is a high potential wild species ideal for the use in rock garden, or as a ground cover in sunny dry areas. This plant has the competence to be developed as a new ornamental species. The purpose of this experiment was to provide an efficient micropropagation protocol for G. aretioides in order to facilitate the availability of this species for further studies of domestication. The influence of various concentrations of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) was investigated for multiplication stage. TDZ at low concentration of 0.05 mg dm−3 resulted in the maximum shoot (9.7) and leaf (42.3) number. The shoots were best rooted on MS medium containing 0.6 mg dm–3 indolebutyric acid (IBA) with 7.8 roots per shoot. Despite achievement of a successful protocol for in vitro multiplication and root induction of Gypsophila, low survival rate was obtained when rooted explants were exposed to ex vitro conditions. This is an important issue, which requires particular consideration and further studies. The possible reasons contributing to the low acclimatization rate of this species are being discussed.
4
Content available In vitro cultures of sclerophyts
100%
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2015
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tom 96
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nr 1
7
Content available Znaczenie minibulw w produkcji ziemniaka w Polsce
84%
EN
Grafting plays an important role in rhododendron propagation. Successful graft union depends on selection of a suitable stock, grafting season, and method. In this study, three methods (whip, cleft, and side grafting) were compared in two seasons. The stocks were mainly cuttings of R. catawbiense-Hybridum, R. ‘Cunningham's White', but also R. pachytrichum and R. brachycarpum. Scions were taken from several cultivars deriving from R. catawbiense. To shorten the production period, scions were grafted on unrooted stocks, and the results are encouraging. The best stock was R. ‘Cunningham's White', especially when side grafting was applied in spring.
EN
Isatis concstricta Davis (an endemic plant of Turkey) suffers from low propagation rates under natural conditions and is threatened due to fast unplanned urbanisation. The study compared the effects of variants of BA + NAA on shoot regeneration on leaf and petiole explants excised from one week old in vitro regenerated seedlings. MS medium containing 1 mg L–1 BA + 1 mg L–1 NAA induced maximum proliferation on petiole and leaf explants with 13.33 and 12.75 shoots per explant repectively. However, leaf explant induced shoots were sturdy and healthier compared to petiole explant induced shoots. These shoots were rooted on MS medium containing 0.5 mg L–1 IBA and the plants were acclimatized in peat moss and sand (v/v). They grew to flowering under ex vitro conditions. This system of regeneration is advantageous for conventional propagation and the results will help in establishment of a powerful and meaningful micropropagation system for I. constricta.
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tom 02
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nr 1
73-83
EN
Bases of young shoots were treated with the Ukorzeniacz B2, preparation stimulating adventitious roots development. Then piles of a mixture of white peat and hortisol were made around the shoots. The shoot growth was accompanied by increasing the height of piles. After one vegetative period the rooted layers were removed off from half of the piled plants, and after two vegetative periods – from the other plants. The quality of the rooted layers was estimated and in spring 1998 and 1999 new plants grew from them. On average 85.2% of Paeonia lactiflora Pall. shoots piled over one vegetative period, and 75% of those piled over two vegetative periods took roots. A higher rooting quality was recorded in two-year layers. Regardless of the piling period, new plants were developed from the all rooted layers in ‘Avelanche’, ‘Lady Dormouth’ and ‘Reine Hortense’ cultivars. These and other cultivars whose layers rooted in over 70% cases, i.e. ‘Baroness Schröder’, ‘La Rosière’, ‘Marechal McMahon’, ‘Monsieur Jules Elie’, ‘Profesor Wóycicki’, ‘Thérèse’ and ‘Ursynów’, can be propagated with vertical layers.
PL
Nasady młodych, wyrastających pędów traktowano preparatem Ukorzeniacz B2, stymulującym rozwój korzeni przybyszowych. Następnie wokół pędów usypano kopczyki z mieszanki torfu wysokiego i gleby ogrodowej. W miarę wzrostu pędów kopczyki podwyższano do ostatecznej wysokości 25 cm. Po jednym okresie wegetacyjnym odcięto ukorzenione odkłady z połowy roślin kopczykowanych, a po dwóch okresach wegetacyjnych – z pozostałych roślin. Oceniono jakość ukorzenionych odkładów, a wiosną 1998 i 1999 roku wyrosłe z nich nowe rośliny. Pędy piwonii kopczykowane w ciągu jednego okresu wegetacyjnego ukorzeniły się przeciętnie w 85,2%, a kopczykowane dwa okresy wegetacyjne – w 75%. Lepszą jakością ukorzenienia charakteryzowały się odkłady dwuletnie. Z wszystkich ukorzenionych odkładów, niezależnie od czasu trwania kopczykowania nowe rośliny rozwinęły się u odmian ‘Avelanche’, ‘Lady Dormouth’ i ‘Reine Hortense’. Odmiany te oraz inne, których odkłady ukorzeniły się w przeszło 70%, tj. ‘Baroness Schröder’, ‘La Rosière’, ‘Marechal McMahon’, ‘Monsieur Jules Elie’, ‘Profesor Wóycicki’, ‘Thérèse’ i ‘Ursynów’ można rozmnażać za pomoc odkładów pionowych.
EN
Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25–1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.
17
67%
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nr 2
541-550
PL
Badania prowadzone w latach 2003-2005 określiły wpływ miejsca i rodzaju podłoża na ukorzenianie sadzonek traw ozdobnych. Sześć gatunków traw: Calamagrostis x acutiflora (SCHRADER) ROCH., Deschampsia caespitosa (L.) P. BEAUV., Festuca ampla HACK., Festuca ovina ‘Glauca’, Spodiopogon sibiricus TRIN., Sesleria tatrae (DEGEN) DEYL rozmnażano przez dzielenie na 1-3 pędowe sadzonki i ukorzeniano w gruncie, w rozstawie 15 x 15 cm oraz w wielodoniczkach w szklarni i w nieogrzewanym tunelu foliowym. Podłożami do ukorzeniania były: torf wysoki, torf wysoki i perlit (1 : 1), torf wysoki i piasek (1 : 1) oraz substrat kokosowy. W szklarni i w nieogrzewanym tunelu foliowym ukorzeniało się średnio 46 i 43% sadzonek, a o ponad połowę mniej na poletkach w gruncie (18%). Mimo mniejszej liczby sadzonek ukorzenionych w gruncie, ich jakość (świeża masa i liczba korzeni, liczba i długość liści), u większości badanych gatunków traw, była lepsza w porównaniu do ukorzenianych w szklarni i w nieogrzewanym tunelu foliowym.
EN
A study was conducted in the years 2003-2005 to examine the influence of a place of rooting and a kind of medium on rooting of ornamental grass cuttings. Six species of ornamental grasses: Calamagrostis x acutiflora (SCHRADER) ROCH., Deschampsia caespitosa (L.) P. BEAUV., Festuca ampla HACK., Festuca ovina ‘Glauca’, Spodiopogon sibiricus TRIN., Sesleria tatrae (DEGEN) DEYL were propagated through one-three stem cuttings and rooted in the soil at a spacing of 15 x 15 cm and in multi-pots in a greenhouse and a foil tunnel. Mediums used for rooting were: peat, peat and perlite (1 : 1), peat and sand (1 : 1) and coco substrate. 46 and 43% of cuttings rooted on the average in a greenhouse and tunnel respectively and half less in the soil (18%). Despite lower number of rooted cuttings in the ground their quality (fresh matter and number of roots, number and length of leaves), with most of the examined grasses, was better in comparison to those rooted in a greenhouse and a foil tunnel.
PL
Celem pracy było określenie warunków mikrorozmnażania kocanek piaskowych. Na etapie namnażania stwierdzono istotny wpływ zastosowanego poziomu BA na liczbę pędów otrzymanych z jednego eksplantatu, natomiast wpływ pożywki podstawowej był nieistotny. Na pożywce MS z dodatkiem 1,0 mg·dm⁻³ BA uzyskano 17,59 pędów z jednego eksplantatu. Kocanki w zastosowanych warunkach ukorzeniały się w 96-100%. Świeża masa mikrosadzonek, długość ich pędów i korzeni, zależała od stężenia BA w pożywce zastosowanej do namnażania. Ponad 90% wysadzonych w szklarni sadzonek zaadaptowało się do warunków ex vitro. Po 10 tygodniach uprawy w szklarni rośliny silnie się rozkrzewiły wytwarzając średnio od 6,8 do 11,6 rozgałęzień. Obserwowano także wytwarzanie odrostów korzeniowych.
EN
The aim of the study was to develop the method of micropropagation of yellow everlasting. On the multiplication stage the influence of BA concentration appeared to be essential for the number of shoots obtained from an explant, however the influence of basic mediums was not significant. On medium MS with 1.0 mg·dm⁻³ BA 17.59 shoots were obtained from one explant. Everlasting microcuttings in the applied conditions were rooting in 96-100%. The fresh weight of microcuttings, their shoot and root length depended on the BA concentration in the multiplication medium. Above 90% of all cuttings planted in a greenhouse were adapted to ex vitro conditions. Over 10 weeks of cultivation in a greenhouse plants branched intensively and formed 6.8-11.6 branches. The root suckers forming was observed.
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