Scanning television optical microscope is designed to investigate microscopic objects, larger than 0.1 micrometers. To illuminate microobject with this microscope there is used a high resolution cathode ray tube. Low illumination of the tested microobject lets to explore living microorganisms in real time. The current microscopic image has low contrast. To increase image contrast it is necessary to increase microscope sensitivity. The main sources of noise in scanning television optical microscope are: 1) composite video shaper; 2) photomultiplier tube; and 3) scanning cathode ray tube. Detailed analysis of noise sources in video signal of the microscope is presented in the paper. Equations describing the sources are given. Dependencies are plotted and discussed.
The high sensitivity time correlated single photon counting system (with photomultiplier tube) for biomedical measurements was designed and tested. Photon time of flight through a parafine wax phantom was modeled by Monte Carlo simulation and next measured by the device. Mean time delay introduced by a phantom is in a good agreement with simulation results. The results presented in this paper demonstrate the feasibility of measuring the optical response with the device.
Ionizing radiation detection in harsh environment conditions often requires additional signal processing to match the requirements of the commercial data readout systems. The subject of this paper is the design of the high-temperature (HT) signal conditioning module that ensures the applicability of scintillation detectors that utilize photomultiplier tubes (PMT) with moderate sampling rate instrumentation. The design was developed for the operation in HT environments (up to 120°C). In order to achieve the optimal signal shape, the module combines a charge amplifi er and a low-pass fi ltering circuitry. An embedded power supply section makes it a complete, standalone unit requiring a single 12 V supplying line. A comprehensive analysis of the developed device, named “PreAmp Shape”, was conducted in order to prove the intended functionality over the different working conditions.
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The article is devoted to the development of the apparatus “Spark” for integration a photodetector with a current output in a measurement system. Developed apparatus “Spark” includes a current-to-voltage converter, an output filter, and a source of a high voltage, that are sufficient to combine a photomultiplier tube with a different analytical instrumentation. Use of apparatus “Spark” with electrochemical station CHI 800C CH Instrument and photomultiplier tube CR-105 by Hamamatsu Photonics is shown by over an electrochemiluminescent assay of a test solution.
PL
W artykule opisano urządzenie “Spark” przeznaczone do pomiarów luminescencji. Urządzenie wyposażone jest w fotodetektor przetwornik prąd-napię, filtr wyjściowy i źródło wysokiego napięcia do współpracy z fotopowielaczem.
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The work is devoted to the development of the apparatus "Pulsar" that is a high-speed pulse counter with a dual interface. It is based on the modern ARM processor STM32F407. High peripheral modules integrations in the selected microcontroller and its productivity were used as a base to PMT integration in electrogenerated chemiluminescent (ECL) analytical systems. Apparatus testing with real ECL compositions showed its capability. ECL pulse response up to 84 MHz or 6 ns pulse duration is transformed to output counting data in two forms for digital and analog interfaces. High temporal resolution up to 10 μs supports measurement techniques based on fast electrode polarization. The built-in double interface simplifies system integration that well demonstrated, as an example, a combination of PMT module H 10682-210 (Hamamatsu Photonics, Japan) with potentiostat Methrohm Autolab 128N.
PL
Praca poświęcona jest opracowaniu aparatu "Pulsar", czyli szybkiego licznika impulsów z podwójnym interfejsem. Oparty jest on na nowoczesnym procesorze ARM STM32F407. Integracja modułów peryferyjnych w wybranym mikrokontrolerze i jego produktywność posłużyły jako podstawa integracji PMT w systemach analitycznych elektrogenerowanych chemiluminescencyjnych (ECL). Testy aparatów z prawdziwymi kompozycjami ECL wykazały jego możliwości. Odpowiedź impulsowa ECL do 84 MHz lub 6 ns czasu trwania impulsu jest przekształcana na wyjściowe dane zliczające w dwóch postaciach dla interfejsów cyfrowych i analogowych. Wysoka rozdzielczość czasowa do 10 μs obsługuje techniki pomiarowe oparte na szybkiej polaryzacji elektrod. Wbudowany podwójny interfejs upraszcza integrację systemu, co dobrze zademonstrowało, jako przykład, połączenie modułu PMT H 10682-210 (Hamamatsu Photonics, Japonia) z potencjostatem Methrohm Autolab 128N.
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