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nr 12
747-750
EN
The studies were done on 90 carps Ki of a 60-80 g weight exposed to permethrin at a concentration of 0.3 pg/L of water for 28 days (group 1) or 11 pg/L for 14 days (group 2). Immunological examinations were done with the blood of fish in group 1 after 4, 7, 14, 21, and 28 days, and in group 2 after 4, 7 and 14 days after the use of permethrin. Immunological examinations were performed after 7 and 14 days from the end of permethrin action. Group 3, untreated, served as a control. It was found that permethrin at a concentration of 0.3 pg/L significantly decreased metabolic activity of neu- trocytes and the level of blood lysozyme after 28 days of exposure. Permethrin at a concentration of 11 pg/L caused death of about 50% of the examined individuals after 14 days exposure. Granulocytosis, monocytosis and unphy- siological nuclear division in neutrocytes and lymphocytes were noted. During the whole period a significantly decreased metabolic activity of neutrocytes and blood plasma lysozyme level were observed. After transport of the fish into clean water, the examined parameters returned to physiological value in group 1 after 14 days, while in group 2 all the parameters had lower values than those in the control group.
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tom 45
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nr 1-2
145-150
PL
Zbadano oporność na permetrynę prusaków odłowionych w obiektach służby zdrowia w niektórych miejscowościach na terenie Polski. Badania prowadzono metodą kontaktową. Oporność badanych owadów była zróżnicowana: wartości LT50 wahały się od 19,1 min. do 1582,9 min.; LT90 - od 75,3 min. do 16066,3 min.
EN
Resistance to permethrin was tested in cockroaches caught in health service institutions in various parts of Poland. The tests were carried out by the contact method as recommended by the WHO. The density of the insecticide was 20 mg/m2. The experiment was done only on male insects, calculating the values of LT50 and LT90 in insect groups caught in various localities. These values were compared with LT50 and LT90 values in sensitive insects exposed to the insecticide, and the resistance indices R50 and R90 were calculated. This made possible determination of permethrin resistance of the populations from various areas. The results showed that the sensitivity of the insects varied. Cockroaches caught in Opole, Biała Podlaska, Szczecin, Warsaw were highly resistant to permethrin, while those from Płock, Piotrków Trybunalski, Chojnów, Nowy Sącz tolerated the insecticide, and those from Jędrzejów were as sensitive as control laboratory insects.
EN
Innovative textile materials with insecticidal and repellent properties can be used in many areas of the textile industry (sportswear, children’s thermoactive clothing, combat uniforms, tents, sleeping bags, mosquito nets, protective clothing for foresters and hunters etc.). In this study, the chemical treatment of polyamide (PA) and polyamide-polypropylene (PA/PP) knitwear with permethrin, a third generation insecticide from influenza synthetic pyrethroids, which is toxic to insects but harmless to humans, was undertaken (Sanitized AM®23-24 preparation was used). In addition to the active substance, the bath contained a cross-linking agent (Appretan®N92111) and softening agent (Solusoft®FE). The content of active substance in the textile product after the application process was examined in dependence on the concentration of the preparation in the bath. When using the right concentration of permethrin and auxiliaries, the fabrics after washing 20 times still showed an effective concentration of insecticide.
PL
Innowacyjne materiały włókiennicze o właściwościach insektycydowych i repelentnych mogą znaleźć zastosowanie w wielu dziedzinach przemysłu włókienniczego (odzież sportowa, dziecięca, termoaktywna, umundurowania bojowe, namioty, śpiwory, moskitiery, odzież ochronna dla leśników i myśliwych). Przeprowadzono chemiczną obróbkę dzianin poliamidowych (PA) i poliamidowo-polipropylenowych (PA/PP) permetryną, insektycydem trzeciej generacji z grypy syntetycznych pyretroidów, toksycznym dla owadów i nieszkodliwym dla człowieka (zastosowano preparat Sanitized®AM 23-24). Kąpiel oprócz substancji czynnej zawierała środek sieciujący (Appretan®N92111) oraz środek zmiękczający (Solusoft®FE). Zbadano zawartość substancji czynnej w wyrobie włókienniczym po procesie aplikacji w zależności od stężenia preparatu w kąpieli. Przy zastosowaniu odpowiedniego stężenia permetryny i środków pomocniczych badane tkaniny, po 20-sto krotnym praniu nadal wykazywały pożądane ilości środka insektycydowego.
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1991
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tom 37
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nr 1
EN
Each generation of Blattella germanica were treated with a dose of 0.42 μg permethrin per VI instar larva. The fecundity varied from 8.21 to 33.66 for I instar larvae per female. Reproduction was significantly lower than that evaluated for untreated insects in third and fourth generations, but significantly higher in the fifth generation. Mortality among insects treated with permethrin, with the exception of the fourth generation only, was higher than among untreated cockroaches. In the third; fourth and fifth generations, the biotic potential was significantly lower in groups of treated insects than in untreated ones. The length of the developmental period of B. germanica treated with permethrin, measured during 7 generations, showed no significant changes in comparison with the control groups.
EN
Synthetic pyrethroids commonly used for plant protection often contaminate superficial water sources and therefore they become a health threat for fish. The objective of the studies was to evaluate the influence of permethrin present in water on phagocytic activity of neutrocytes macrophage/monocytes of the main kindneys and circulatory blood of the carp. The fish of about 90 ±10 g were exposed for 96 h to permethrin at a concentration of 0.03 and 1.1 µg/L in the insecticide Ambusz 25EC. The percentage of phagocytic cells (KF) among neutrocytes and macrophages, the value of phagocytic index (IF) and metabolic activity of phagocytes (NBT) were determined in the kidneys and in blood of 6 individuals from each experimental group at 1, 3, 4, 7 and 14 days after the end of the experiment. The same parameters have been evaluated three times for controls at day 1, 7 and 14 of the experiment. The 96-hour exposure of the carp to permethrin during the experiment induced leukopenia and neutrocytosis and lowered the percentage of the kidney cells (KF) active in phagocytosis. Compared to control, at day 7 the value of phagocytic index in the fish under experimentation also decreased.
PL
Porównano wpływ permetryny i fenwaleratu na indukcję zmian genetycznych w komórkach somatycznych i płciowych samców myszy łaboratoryjnych przy różnych drogach narażenia. Stwierdzono różnicę w oddziaływaniu ww. pyre- troidów na oceniane komórki w zależności od drogi podania.
EN
Synthetic pyrethroid insecticides are widely used in the protection of fruits and vegetables as well as in the public hygiene due to their strong neurotoxic activity against insects. The induction of genetic changes in somatic and sex cells in male mice after different routes of exposure to permethrin and fenvalerate was studied. The male 8-10 weeks old mice were intraperitoneally exposed to 20 and 40 mg/kg bw of fenvalerate and 125 and 250 mg/kg bw of permethrin. Another groups of mice were exposed per os to fenvalerate and permethrin in the doses of 50, 100 and 200, 400 mg/kg bw respectively. For the sperm anomalies testing the exposure was repeated for five consecutive days followed by the 35 days waiting period after which the gonads were removed and spermatozoa prepared from the epididymis. The changed spermatozoa were counted in the smears after staining in the 0.5% eosin Y solution and the results compared with the number of normal cells. For the testing of the effect of pyrethroids on the micronuclei frequency in the bone marrow cells the tested substances were administered twice in 24 hours intervals and the bone marrow was sampled after 6 and 24 hours from the femur bone. The polychromatic erythrocytes and the presence of micronuclei were evaluated in the bone marrow smears. The results showed the difference in the action of the pyrethroids on the genetic material of the tested cells and the effect of the route of exposure. Permethrin induced the lesions in the sex cells regardless the route of exposure, however a substantial increase in the micronuclei frequency in the bone marrow was observed after oral exposure only. No signs of cytotoxicity accompanied the sperm anomalies and micronuclei induction. Fenvalerate induced changes in sperm cells after intraperitoneal exposure only. No increase in the micronuclei frequency in the polychromatic erythrocytes of the bone marrow was observed after per os or intraperitoneal exposure. The intraperitoneal exposure to this pyrethroid resulted in cytotoxicity in both bone marrow and sex cells.
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tom 48
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nr 3
229-237
PL
Porównano wpływ pestycydów: DDT i permetryny na indukcję form molekularnych cytochromu Р-4501A i 2B. Stwierdzono, że permetrynę podobnie jak DDT; zaliczyć można do induktorów cytochromu P-4502B-typu fenobarbitalu.
EN
The effect of permethrin on relative liver weight (RLW) and the activity of hepatic monooxygenase system related to cytochrome P-4502B and 2A was studies. The effect of permethrin was compared with DDT used as phenobarbital-type of monooxygenase inducer (induces cyt. P-4502B). Male Wistar rats received permethrin and DDT for 4 days at 24 h intervals in daily oral doses of 1/10, 1/50 and 1/100 LD50. 3-methylocholantrene and phenobarbital which served as inducers of cytochrome P-4501A and 2B, respectively and were used as positive controls. The activities of cytochrome(s) P-450 were measured by 7-pentoxy- and 7-etoxyresofurin O-dealkylation by S-9 fraction of rat liver; these two compounds have been shown to be the substrates for reactions mediated by cytochrome P-4502B and 2A. Thus this biochemical procedure permits to determine whether tested compound belongs to one of two main types of inducers of the cytochrome P-450 monoxygenase system. Treatment of rats with both pesticides resulted in significant increase in RLW, to 30 and 15% of control, respectively. In animals treated with permethrin the metabolism of 7-pentoxyresofurin increased in a dose dependent manner. Phenobarbital and the highest dose of permethrin (620 mg/kg b.w. x day-1) induced similar (about 30-fold) increase in O-dealkylation of 7-pentoxyresofurin. DDT stimulated metabolism of 7-pentoxyresofurin to much higher degree as compared with phénobarbital. It should be noted that both pesticides induced only slight increase in O-dealkylation of 7-etoxyresofurin (cyt. P-4501A-mediated reaction). The present results indicate that permethrin as well as DDT shows the ability to induce the phenobarbital-type of cytochrome P-4502B.
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