Wyniki wyszukiwania - Biblioteka Nauki

1

Very High Concentration of D-Dimers in Portal Blood in Patients with Pancreatic Cancer

100%

Durczyński A. , Szymański D. , Nowicki M. , Hogendorf P. , Poznańska G. , Strzelczyk J.

Polish Journal of Surgery

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2012

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tom 84

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nr 10

521-525

EN

Nowadays, increasing attention has been focused on relation between increased D-dimer levels and cancer among patients without detectable thrombosis. was to measure plasma D-dimer levels in portal and peripheral blood in pancreatic cancer patients with absence of venous thromboembolism. Material and methods. Fifteen consecutive patients hospitalized in the Department of General and Transplant Surgery of Medical University in Łódź, from January to March 2012 who underwent surgery due to a pancreatic cancer were enrolled. At laparotomy, portal and peripheral blood were sampled concurrently. D-dimer and fibrinogen levels were measured. Moreover, to investigate overall coagulation function prothrombin time (PT), prothrombin index (PI), international normalized ratio (INR), thrombin time (TT), activated partial thromboplastin time (APTT), TT and APTT index were evaluated. Results. Peripheral plasma D-dimmer levels above normal range were found in 10/15 patients (66,67%), whereas D-dimer above normal values were confirmed in all portal blood samples. Mean D-dimer values were higher in portal than in peripheral blood (3279.37 vs 824.64, by 297%, p=0,025). These discrepancies were accompanied by normal limits of portal and peripheral levels of fibrinogen and comparable coagulation function indexes. Conclusion. Our preliminary study showed the close relation between activation of hemostasis, reflected by elevated D-dimers in portal blood and presence of pancreatic cancer. These data suggest that measurement of portal blood D-dimer levels may be a potentially useful technique for screening the pancreatic cancer.

2

Oxidative metabolism of peripheral blood neutrophils in chronic renal failure on conservative and dialysis treatment

100%

Luciak M.

Archivum Immunologiae et Therapiae Experimentalis

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1992

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tom 40

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nr 2

3

Proportion of cytotoxic peripheral blood natural killer cells and T-cell large granular lymphocytes in recurrent miscarriage and repeated implantation failure: case–control study and meta-analysis

88%

Kolanska K. , Suner L. , Cohen J. , Kraiem Y. B. , Placis L. , Fain O. , Bornes M. , Sellert L. , Delhommeau F. , Feger F. , d'Argent E. M. , Darai E. , Chabbert-Buffet N. , Antoine J.-M. , Kayem G. , Mekinian A.

Archivum Immunologiae et Therapiae Experimentalis

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2019

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tom 67

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nr 4

4

Two new cases of KIR3DP1, KIR2DL4-negative genotypes, one of which is also lacking KIR3DL2

88%

Niepieklo-Miniewska W. , Zuk N. , Dubis J. , Kurpisz M. , Senitzer D. , Havrylyuk A. , Grendziak R. , Witkiewicz W. , Chopyak V. , Kusnierczyk P.

Archivum Immunologiae et Therapiae Experimentalis

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2014

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tom 62

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nr 5

5

Histamine receptor expression on peripheral blood lymphocytes is influenced by specific and nonspecific activation

88%

Zak-Nejmark T. , Kraus-Filarska M. , Malolepszy J. , Jankowska R. , Jutel M. , Nowak I. A. , Nadobna G.

Archivum Immunologiae et Therapiae Experimentalis

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2000

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tom 48

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nr 2

6

Detection of circulating breast cancer cells in peripheral blood by a two-marker reverse transcriptase-polymerase chain reaction assay

88%

Fabisiewicz A. , Kulik J. , Kober P. , Brewczynska E. , Pienkowski T. , Siedlecki J. A.

Acta Biochimica Polonica

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2004

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tom 51

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nr 3

EN

The aim of this study was to use a two-marker assay for the detection of breast cancer cells circulating in patients' blood. We have applied a PCR-based methodology to follow up the possibility of the development of metastatic disease in stage I and II patients who had undergone curative surgery. Since the number of circulating cancer cells in peripheral blood is very low, the technique for their detection needs to be not only highly sensitive, but also very specific. The reverse transcriptase-polymerase chain reaction (RT-PCR) technique may improve the sensitivity of breast cancer cell detection up to only a few cells per one million. The principle of the RT-PCR assay is to amplify a messenger RNA characteristic for breast epithelial cells in a blood sample. Since we do not expect such cells to be circulating in peripheral blood of healthy subjects, detection of the characteristic mRNA should indicate the presence of circulating breast cancer cells. We analyzed the usefulness of three mRNA markers: cytokeratin 19 (CK19), mammaglobin (hMAM) and S subunit of human chorionic gonadotropin (S-hCG) for this test. Blood samples (112) were obtained from 55 patients, in stages I and II, with or without metastasis to regional lymph nodes (N0 or N1). We found that a two-marker assay increases the sensitivity of detection of breast cancer cells in comparison with a single-marker one. Combination of two tumor-specific mRNA markers, hMAM/CK19 or S-hCG/CK19, allowed the detection of circulating breast cancer cells in 65% of N1 patients and 38% of N0 patients. By comparison, the combination hMAM/-hCG allowed the detection of circulating breast cancer cells in the blood of 68% of N1 patients and 46% of N0 patients. Addition of the third marker did not significantly increase the detection sensitivity.

7

Endothelial progenitor cells as a new agent contributing to vascular repair

88%

Miller-Kasprzak E. , Jagodzinski P. P.

Archivum Immunologiae et Therapiae Experimentalis

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2007

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tom 55

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nr 4

8

CTLA‑4 expression inversely correlates with kidney function and serum immunoglobulin concentration in patients with primary glomerulonephritides

75%

Grywalska E. , Smarz-Widelska I. , Mertowski S. , Gosik K. , Mielnik M. , Podgajna M. , Abramiuk M. , Drop B. , Rolinski J. , Zaluska W.

Archivum Immunologiae et Therapiae Experimentalis

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2019

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tom 67

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nr 5

9

Evaluation of peripheral blood leukocyte subpopulations by flow cytometry in calves treated with Mannheimia haemolytica leukotoxin

75%

Bednarek D. , Urban-Chmiel R. , Dudek K. , Szymanska-Czerwinska M.

Bulletin of the Veterinary Institute in Puławy

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2009

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tom 53

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nr 2

199-203

EN

The aim of the study was to evaluate the effect of Mannheimia haemolytica leukotoxin (Lkt) on cellular immune response in clinically-healthy calves given intravenously 25 µg per animal of M. haemolytica Al Lkt. The alternations of peripheral blood leukocytes were examined with a flow cytometry. The Lkt treated calves were compared with the non-treated controls before (0) and 1, 2, 3, 4, 5, 6, and 24 h after the treatment. The following parameters were assayed: white blood cell (WBC) count, percentage of polymorphonuclear leukocytes (PMNL), mid-size leukocytes, total percentage of lymphocytes and their subsets: CD2⁺ (T lymphocytes), CD4⁺ (T helper lymphocytes), and CD8⁺ (T suppressor/cytotoxic lymphocytes) with CD4⁺: CD8⁺ratio, and also WC4⁺ cells (B lymphocytes). The obtained results showed that in the treated calves, the WBC count, the percentages of PMNL, mid-size leukocytes, and some subpopulations of peripheral lymphocytes (CD2⁺, CD4⁺, CD8⁺) were significantly lower compared with the controls during the first of three hours of the experiment, and then the parameters increased and returned to the physiological level.

10

Flow cytometric analysis of CD133- and EpCAM-positive cells in the peripheral blood of patients with lung cancer

75%

Skirecki T. , Hoser G. , Kawiak J. , Dziedzic D. , Domagala-Kulawik J.

Archivum Immunologiae et Therapiae Experimentalis

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2014

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tom 62

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nr 1

11

Flow cytometric assay in peripheral blood of dogs - reference values for leukocytes from Brazilian Beagles

75%

Fujiwara R. T. , Bueno L. L. , Vale A. M. , Franca-Silva J. C. , da Costa R. T. , da Silva Quetz J. , Machado-Coelho G. L. , Reis A. B. , Martins Filho O. A. , Genaro O. , Nascimento E. , Mayrink W.

Polish Journal of Veterinary Sciences

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2005

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tom 08

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nr 1

12

Effect of beta-1,3-1,6-D-glucan on the phagocytic activity and oxidative metabolism of pripheral blood granulocytes and monocytes in rats

75%

Wojcik R. , Janowska E. , Malaczewska J. , Siwicki A. K.

Bulletin of the Veterinary Institute in Puławy

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2009

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tom 53

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nr 2

241-246

EN

The experiment was performed on 20 adult Wistar rats aged 12 weeks, divided into two equal groups (control and experimental), each comprised of five males and five females. From the first day of the experiment, the experimental group rats were fed Murigran feed supplemented with ß-l,3/l,6-D-glucan at a dosage of 12-19 mg/rat/d, subject to body weight, while the control- group was administered the same feed without any additives. At the beginning of the experiment and then after 14 days, arterial blood samples were collected from the rats and diluted with heparin to measure and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. Statistically higher levels of the activity were observed in the group of rats administered glucan than in controls, expressed in terms of the percentage of phagocytic cells as well as average fluorescence intensity. ß-l,3/l,6-D-glucan also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with E. coli, and on the oxidative metabolism of granulocytes after stimulation with PMA.

13

The effect of a single dose of sheep's erythrocytes on the activity of acid phosphatase in lymphocytes of peripheral blood in bursectomized chickens

75%

Graczyk S.

Folia Histochemica et Cytobiologica

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1984

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tom 22

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nr 2

14

The influence of moderate-intensity physical effort on peripheral blood in adults with Down syndrome - a pilot study

75%

Aleksander-Szymanowicz P. , Marchewka A. , Dabrowski Z. , Teleglow A. , Bac A. , Glodzik J.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 5

15

Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test

75%

Kopjar N. , Želježić D. , Garaj-Vrhovac V.

Acta Biochimica Polonica

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2006

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tom 53

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nr 2

321-336

EN

The present study was undertaken to contribute to the characterization of the degree of variability in baseline damage in white blood cells from control population, and to investigate how this variability is associated with external and internal factors. Altogether 170 healthy volunteers, randomly selected from the general population of the Republic of Croatia, participated in the study. Two sensitive tests: the alkaline comet assay and the chromosome aberration test were applied to study the background levels of DNA damage in their white blood cells. The results point to inter-individual differences, indicating different genome sensitivity. As revealed by both assays, the background levels of DNA damage were mostly influenced by smoking habit as well as medical exposure (especially to diagnostic X-rays). Sex and age of subjects did not significantly influence the values of DNA damage recorded in the white blood cells. Although higher levels of DNA damage were recorded in blood samples collected during winter and autumn, they were mostly influenced by medicinal exposure and smoking habit. Statistical evaluation of the data confirmed that a positive correlation exists between DNA migration and the number of long-tailed nuclei found with the comet assay and the total number of chromosome aberrations. The data obtained can serve as control values in forthcoming biomonitoring studies.

16

Dose- and time-dependent micronucleus induction in peripheral erythrocytes of catfish, Heteropneustes fossilis (Bloch) by zinc

75%

Talapatra S. N. , Banerjee P. , Mukhopadhyay A.

International Letters of Natural Sciences

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2014

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tom 04

EN

As far as the detection of metal genotoxicity in fish is concerned, micronucleus (MN) test is considered an extremely suitable measure. In this study, frequencies of micronucleated erythrocytes were scored in peripheral blood of catfish, Heteropneustes fossilis (bloch) after acute in-vivo exposure of zinc at different concentrations (5, 10 and 30 ppm) in the laboratory condition. These three concentrations of zinc were tested at different durations such as 24h, 48h, 72h and 96h respectively. Highly significant (P < 0.001) increased values were obtained for MN frequencies in the peripheral erythrocytes of exposed fishes compared to control groups of fishes. These results confirm that dose- and time-dependent micronucleation in the peripheral erythrocytes of fish after short-term exposure to zinc could provide valuable information regarding zinc containing effluent quality and also help in genetic biomonitoring with this test model. In this context safe concentration of zinc vis-a-vis genotoxicity range could be evaluated for future studies.

17

In vitro effects of prolonged exposure to quercetin and epigallocatechin gallate of the peripheral blood mononuclear cell membrane

75%

Margina D. , Ilie M. , Manda G. , Neagoe I. , Danciulescu-Miulescu R. , Purdel C. N. , Gradinaru D.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 4

EN

The study aimed to assess biophysical changes that take place in the peripheral blood mononuclear cell (PBMC) membranes when exposed in vitro to 10 μM quercetin or epigallocatechin gallate (EGCG) for 24 and 48 h. PBMCs isolated from hypercholesterolemia patients were compared to those from normocholesterolemia subjects. The membrane fluidity and transmembrane potential were evaluated and the results were correlated with biochemical parameters relevant to oxidative stress, assessed in the patients’ plasma. The baseline value of PBMC membrane anisotropy for the hypercholesterolemia patients was lower than that of the control group. These results correlated with the plasma levels of advanced glycation end products, which were significantly higher in the hypercholesterolemia group, and the total plasma antioxidant status, which was significantly higher in normocholesterolemia subjects. In the case of normocholesterolemia cells in vitro, polyphenols induced a decrease in membrane anisotropy (7.25–11.88% at 24 h, 1.82–2.26% at 48 h) and a hyperpolarizing effect (8.30–8.90% at 24 h and 4.58–13.00% at 48 h). The same effect was induced in hypercholesterolemia cells, but only after 48 h exposure to the polyphenols: the decrease in membrane anisotropy was 5.70% for quercetin and 2.33% for EGCG. After 48 h of in vitro incubation with the polyphenols, PBMCs isolated from hypercholesterolemia patients exhibited the effects that had been registered in cells from normocholesterolemia subjects after 24 h exposure. These results outlined the beneficial action of the studied polyphenols, quercetin and EGCG, as dietary supplements in normocholesterolemia and hypercholesterolemia patients.

18

Reduced expression of AURKA in peripheral blood of breast cancer patients

75%

Goh L. P. W. , See E. U. H. , Chua K. H. , Lee P.-C.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2018

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tom 99

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nr 1

19

Analysis of red blood cell parameters in dogs with various stages of degenerative mitral valve disease

75%

Kumiega E. , Michalek M. , Kasztura M. , Noszczyk-Nowak A.

Journal of Veterinary Research

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2020

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tom 64

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nr 2

20

Effect of IL-18 on IL-1beta and sIL-1RII production by human neutrophils

75%

Jablonska E. , Izycka A. , Wawrusiewicz N.

Archivum Immunologiae et Therapiae Experimentalis

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2002

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tom 50

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nr 2