The aim of this study was to investigate the acute effect of spaying on antioxidant defence mechanism and lipid peroxidation in healthy bitches. Thirty-one healthy bitches were used in the study. All the animals were anaesthetised with xylazine-ketamine combination and subjected to total ovariohysterectomy (OVH). The blood glutathione (GSH), plasma malondialdehyde (MDA), ceruloplasmin, β-carotene, and retinol levels were determined in the preoperative and early postoperative periods. There was a significant decrease in blood GSH levels and an increase in plasma MDA activity at early postoperative stage as compared to the stage before the operation. There were no significant differences in the concentration of β-carotene, retinol, and ceruloplasmin levels between the preoperative and early postoperative periods. In conclusion, these findings indicate some alterations in the antioxidant defence system in the early postoperative stage. The assessment of GSH and MDA levels can be useful for the evaluation of oxidative stress after OVH in bitches.
The aim of the study was to determine the degree of phagocytic activity of neutrophils, lymphocyte histogenesis and total immunoglobulin level in six bitches with glandular cystic hyperplasia - pyometra complex (GCH - PC) before and after ovariohysterectomy in comparison to healthy dogs. Haematological study showed significant increases (P<0.005) in the white blood cell count and neutrophilia. After ovariohysterectomy these parameters gradually returned to normal. The phagocytic ability (expressed as phagocytic activity PA and index of phagocytic activity IPA) of neutrophils was impaired in bitches wit GCH - PC and has improved during the therapy. The degree of concanavalin A induced lymphocyte blastogenesis expressed as a stimulation index was significantly (P<0.005) depressed in affected bitches and after surgical treatment had not reached values comparable with that found in healthy dogs. The level of total immunoglobulins was significantly (P<0.05) decreased at the beginning of observation and gradually increased after treatment. The results of the present study have provided usuful information for diagnosis and prognosis of GCH - PC in bitches because the success of the treatment and recovery from the GCH - PC is in direct relation with the optimal condition of the immune system.
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