Wyniki wyszukiwania - Biblioteka Nauki

1

Organophosphate insecticide chlorfenvinphos affects superoxide dismutase, catalase and malondialdehyde in rat liver

100%

Lukaszewicz-Hussain A.

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tom 10

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nr 4

EN

The aim of this paper was to study the activity of liver superoxide dismutase and catalase and the concentration of liver malondialdehyde in rats intoxicated with chlorfenvinphos, an organophosphate widely used as an insecticide. The study was carried out on male Wistar rats weighing 180-230 g. The rats were divided into two groups: examined - receiving oil solution of chlorfenvinphos in doses of 0.5 LD50, and 0.1 LD50, and control group - receiving oil. The activity of superoxide dismutase (SOD), catalase (CAT) and concentration of malondialdehyde (MDA) were determined after 1, 24 and 48 hours of intoxication. We observed an increase in the liver activity of SOD in further period of intoxication with chlorfenvinphos in both doses and a decrease of liver SOD activity in the rats intoxicated with the higher dose. The CAT activity in liver of the treated rats increased at the 1st hour of experiment with a dose of 0.5 LD50 and at the Ist hour and the 24th hour after intoxication with a dose 0.1 LD50. Hepatic concentration of MDA showed a decrease at the 24th hour of intoxication with chlorfenvinphos and an increase at the 48th hour of intoxication with the higher dose and returned to control value for the rats intoxicated with the lower dose. SOD and CAT play a major role in the maintaince of the physiological level of reactive form of oxygen. When reactive oxygen species generation exceeds capability of redox degrading systems, MDA levels increase. The results obtained suggest that reactive oxygen species in liver injury might be caused by chlorfenvinphos.

2

Mitochondrial glutathione-S-transferases from pig thymus and the effect of organophosphate insecticides on their activity

84%

Walter Z. , Wojtysiak-Staniaszczyk M. , Przepiorkiewicz M. , Kargas C. , Gawronska M. , Grabowska M. , Majsterek I.

Acta Biologica Cracoviensia. Series Zoologia

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2001

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tom 43

3

Liver catalase, glutathione peroxidase and reductase activity, reduced glutathione and hydrogen peroxide levels in acute intoxication with chlorfenvinphos, an organophosphate insecticide

84%

Lukaszewicz-Hussain A. , Moniuszko-Jakoniuk J.

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nr 3

EN

The aim of this study was to determine the effect of acute intoxication with chlorfenvinphos on the liver reduced glutathione (GSH) level and the activities of two enzymes involved in glutathione metabolism, as well as the activity of catalase (CAT), glucose-6-phosphate dehydrogenase (G6PDH) and the level of liver hydrogen peroxide. The concentration of malondialdehyde (MDA) was determined as lipid peroxidation index. Rats were divided into two groups: the control group, which received oil intragastrically by stomach tube, and the experimental groups, which received oil solution of chlorfenvinphos (CVP) in doses of 0.02 LD50, 0.1 LD50 or 0.5 LD50. After 1, 24, 48 hours of intoxication with chlorfenvinphos, the livers were quickly removed and placed in iced 0.9% NaCl containing 0.16 mg/ml heparin. The liver glutathione peroxidase (GPx) and reductase (GR) activities as well as GSH and hydrogen peroxide levels were determined using Bioxytech Assay kits. CAT activity was determined by Aebi method, G6PDH activity was measured using Sigma Assay kit. MDA level was determined by Buege and Aust method. The changes of examined parameters of antioxidative system as well as lipid peroxidation index were found. The correlation between MDA concentration and the elements of enzymatic and non-enzymatic antioxidative system was determined. A statistically significant correlation was found only between MDA level and G6PDH activity. In conclusion, it is suggested that G6PDH play a key role in the defence against oxidative stress induced by intoxication with chlorfenvinphos.

4

The influence of pretreatment with N-acetylcysteine on serum cholinesterase activity and liver glutathione levels in rats intoxicated with chlorfenvinphos

84%

Lukaszewicz-Hussain A. , Moniuszko-Jakoniuk J.

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nr 1

EN

This study was intendent to examine if N-acetylcysteine (NAC) changes liver GSH levels and influences ChE serum activity in rats intoxicated with chlorfenvinphos. The studies were conducted on male Wistar rats of 200±20 g body weight. One group of rats was pretreated with 0.1% water solution of NAC. NAC was administered in drinking water 24h before intoxication. The control groups received oil intragastrically by stomach tube in the amount of 0.1ml/100g (I), immediately or after NAC pretreatment. The experimental groups received oil solution of chlorfenvinphos in a dose of 0.02 LD50 or 0.1 LD50 immediately or after pretreatment with NAC. One and 24 hours after intoxication with chlorfenvinphos (or after NAC pretreatment) the blood samples were collected and livers were quickly removed and placed in iced 0.9% NaCl containing 0.16 mg/ml heparin. ChE serum activity and GSH level were measured. The results of this study demonstrated the changes in serum ChE activity and liver glutathione levels in the rats after administration of chlorfenvinphos at single doses. The results reported here indicate that NAC influences a decreased level of GSH in the liver of chlorfenvinphos-intoxicated rats and does not prevent ChE inhibition.

5

Effect of insecticide Owadofos EC-50 on Spodoptera exigua larvae

67%

Fila K. , Adamski Z. , Ziemnicki K.

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tom 34

6

Efekty toksycznego dzialania olowiu i wybranych insektycydow fosforoorganicznych w organizmie szczura. III. Wplyw olowiu na zawartosc pozostalosci fenitrotionu oraz foschloru w watrobie i mozgu szczurow

51%

Barski D. , Zasadowski A.

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tom 25

115-123

PL

Badano zmiany stężenia pozostałości insektycydów fosforoorganicznych w wątrobie i mózgu szczurów po intoksykacji pojedyńczej fenitrotionem, foschlorem oraz ołowiem z fenitrotionem i ołowiem z foschlorem. Octan ołowiu(II) podany w dawce 250 mg/kg m.c. przed fenitrotionem zarówno w sposób jednorazowy, jak i frakcjonowany (przez 5 dni po 50 mg/kg m.c.) spowodował trwający przez cały okres doświadczenia wzrost stężenia pozostałości tego insektycydu w badanych tkankach zwierząt w porównaniu z zatruwanymi wyłącznie fenitrotionem. Po dawce frakcjonowanej Pb(II) wzrost pozostałości fenitrotionu był większy. W wątrobie i mózgu szczurów, którym przed foschlorem podano, Pb(II) w niektórych okresach eksperymentu stwierdzano obniżony poziom pozostałości zastosowanego insektycydu w porównaniu ze zwierzętami zatruwanymi wyłącznie foschlorem. Zastosowanie ołowiu w dawce frakcjonowanej przed foschlorem w większym stopniu wpłynęło na obniżenie pozostałości tego insektycydu w badanych tkankach w porównaniu z zatruwanymi jednorazową dawką ołowiu i foschlorem.

EN

The concentration changes of phospho-organic insecticides in livers and brains of rats after a single intoxication with fenitrothion, foschlor as well as with combinations of lead and fenitrothion and foschlor, were examined. The lead acetate (II) provided in 250 mg/kg (body weight) prior to fenitrothion application both in a single dosage and fractionated for 5 days at 50 mg/kg a day (body weight) resulted in the increased concentration of this insecticide. The level of fenitrotion was stable throughout the period of examination in the analysed animal tissue when compared with intoxication with fenitrothion individually. The increase in residue was even larger after the fractionated dosage of Pb (II). In the liver and brain of rats intoxicated with Pb(II) prior to intoxication with foschlor, a decreased level of the applied insecticide residue was observed during some periods of the experiment. The lead application in the fractionated dosage prior to intoxication with foschlor resulted in the lowering the insecticide residue level to a larger degree in the examined tissue when compared with the rats intoxicated with a single dosage of lead and foschlor.