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EN
Seasonal changes in the chemical composition of sediments from four stations located in the Pomeranian Bay were analysed. The investigations were carried out in four periods (March and July 1996, and May and October 1997). The following parameters were investigated: organic carbon and nitrogen, total phosphorus and organic phosphorus, loss on ignition and redox potential. On the basis of these results the influence of the following processes and phenomena was noted: the early spring phytoplankton bloom dominated by diatoms (March 1996); the intense inflow of allochthonous matter brought into the Bay with the waters of the Swina (May 1997); the summer bloom of blue-green algae and dinoflagellates (July 1996); the consequences of the summer 1997 flood which occurred in southern Poland (October 1997).
EN
This study presents the results of research on the effect of long-term use of phosphorus fertilizers on permanent sugar beet crops for more than 50 years and on the transformation of phosphate forms on light chestnut soil and its yield. Our work aims to establish the main factors of quantitative and qualitative changes in various phosphates in light chestnut soil. Despite the large amount of practical material, the influence duration of phosphorus fertilizer application has not been sufficiently studied on the irrigated soils of Kazakhstan. It should be noted that the current study was carried out in long-term stationary experimental sites for the production of sugar beet with permanent sowing. The introduction of phosphate fertilizers primarily on the permanent crops of sugar beets in the same norms contributes to a more significant increase in gross phosphorus reserves. The soil content of gross phosphorus for 58 years on the control and nitrogen-potassium variants show practically no changes. Furthermore, when phosphorus fertilizers are applied on the variant with the annual application of a single norm of phosphorus and its amount for 58 years (4400 kg∙ha–1 of application doses) its content increased by 2660 mg∙kg–1, and with the introduction of its one and a half norms (6600 kg of application doses) by 2860 mg∙kg–1 of soil.
EN
The statistical analysis was performed to compare average contents of phosphorus fractions in the bottom sediments between the lacustrine zone and the riverine zone in the Solina Reservoir, between the main object and the retarding one as well as between all the research stations of the analysed reservoirs. It was found that the deposits from the stations localized in the lacustrine zones of both reservoirs did not differ significantly in regards to total phosphorus content, inorganic phosphorus, and non-apatite, inorganic phosphorus fractions, however, they were characterized by significantly different contents of organic phosphorus.
EN
Effects of mesotrophic lake water enrichment with organic phosphorus and nitrogen substrates (DNA and model protein, bovine serum albumin - BSA) on dynamics and diversity of natural microbial communities (bacteria, heterotrophic nanoflagellates, ciliates) were studied in mesocosm experiments. Simultaneous enrichment with DNA and BSA strongly increased the abundance and biomass of all studied groups of microorganisms and induced changes in their morphological and taxonomic structure. The increased participation of large heterotrophic nanoflagellates cells (larger than 10 μm) in their total numbers and shifts in taxonomic and trophic Structure of the ciliates, from algivorous to small bacterivorous, species were observed. Grazing caused changes in bacterial size distribution in all enriched mesocosms. Large (10-50 μm) filamentous bacteria significantly contributed to the total bacterial numbers and biomass. Pronounced increase in populations of (β- and γ-Proteobacteria was found in lake water enriched with organic P and N sources, whereas α-Pmteobacteria did not change markedly in the studied mesocosms. DNA additions stimulated the rates of bacterial secondary production. BSA shortened the rates of bacterial biomass turnover in lake water. Relatively high and constant (~ 30%) percentage contribution of active bacteria (MEM+) in two mesocosms enriched with DNA and DNA+BSA suggested the important role of nucleic acids as a source of phosphorus for bacterial growth, activity and production. Numerous and statistically significant correlations between bacteria and protists indicated the direct and selective predator-prey relationship.
EN
This study presents results on the availability of various organic P compounds for bacteria from mesotrophic Lake Constance. The rates of hydrolysis of all tested compounds added to the analyzed lake water samples did not correlate with assimilation of liberated inorganic P. beta -glycerophosphate and AMP were the most efficiently hydrolysed by bacterial phosphohydrolytic enzymes. The highest specific P uptake was found in water samples supplemented with nucleotides. The fastest increase in bacterial numbers was observed in water samples enriched with DNA, RNA, ATP and phytin. Analysis of discrepancies between rates of hydrolysis, specific P uptake and bacterial growth rates in samples enriched with various organic P compounds suggested that bacterial phosphatases participated substantially in processes of dissolved organic carbon (DOC) compound decomposition in lake water, whereas 5'-nucleotidase was mainly responsible for bacterial P demand.
PL
Celem przeprowadzonych badań było porównanie stosowanych do oznaczania zawartości fosforu ogólnego, nieorganicznego i organicznego w glebie trzech metod: Brogowskiego, Black’a i Olsena oraz Golachowskiej. Podczas badań analizowano również zmiany zawartości fosforu ogólnego, nieorganicznego, organicznego i przyswajalnego w glebach inkubowanych. Do doświadczenia użyto trzech gleb lekkich o składzie granulometrycznym piasku słabogliniastego. W drugiej części badań przeprowadzono inkubację dwóch gleb: piasku słabogliniastego i piasku gliniastego lekkiego przez okres 60 dni. Stosując metodykę proponowaną przez Black’a i Olsena oznaczono największe ilości fosforu ogólnego, nieorganicznego i organicznego. Trwająca 60 dni inkubacja piasku słabogliniastego i piasku gliniastego lekkiego nie wpłynęła na zmianę rozdziału fosforu glebowego pomiędzy część organiczną i nieorganiczną.
EN
The aim of the performed investigations was the comparison of usefulness of various methods for the determination of total, organic and inorganic phosphorus in the soils. The evaluated methods were worked out by Brogowski, Black and Olsen and Golachowska. During the investigations changes in the content of total, organic, inorganic and available phosphorus in the incubated soils were also analyzed. Three sandy soils of granulometric traits of coarse sand were used in the experiment. In the second part of the experiment two soils were subjected to 60 day incubation, namely coarse sand and loamy sand. Using the method suggested by Black and Olsen the highest contents of total, organic and inorganic phosphorus were determined. The 60 day incubation of coarse and light loamy sand did not affect the division between organic and inorganic soil phosphorus.
PL
Niniejszy artykuł zawiera charakterystykę podstawowych organicznych i nieorganicznych związków fosforu występujących w glebach. Jednocześnie omawia w sposób chronologiczny rozwój metod mających na celu określenie ilości tych związków w glebach. Omawiane metody oparte są ekstrakcji gleby roztworami różnych związków chemicznych. Ponadto w artykule przedstawiono opis podstawowych zasad dotyczących metod oznaczania zawartości fosforu przyswajalnego w glebach. Omawiane metody to metody: biologiczne, biologiczno-chemiczne i chemiczne. Odnośnie metod chemicznych przedstawiono przydatność stosowanych roztworów ekstrakcyjnych w porównaniu do warunków istniejących podczas pobierania przez rośliny fosforu z gleby.
EN
The present paper contains a characteristic of basic organic and inorganic phosphorus compounds, appearing in the soil. Meanwhile the paper discusses in a chronological order the evolution of methods aiming at determining the abundance of these compounds in the soil. Described methods are based on soil extraction by the solutions of various chemical compounds. The article presents basic rules of methodology of the determination of available phosphorus in soil. Described methods include biological, bio-chemical and chemical ones. For chemical methods the usability of different extraction solutions, in relation to the conditions of phosphorus assimilation from soil by plants is discussed.
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