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Zastosowanie odczynu fluorescencji w swietle spolaryzowanym w diagnostyce brucelozy bydla

100%

Szulowski K. , Murat J. , Iwaniak W. , Weiner M. , Pilaszek J.

Medycyna Weterynaryjna

2008

tom 64

nr 08

1019-1022

In view of the large scale of surveys, the diagnosis of brucellosis is primarily based on serological examinations. Regarding cattle, RBT, SAT, CFT, Coombs, 2-ME and ELISA are used in Poland. The process is laborious and time-consuming. The problem of differentiation between positive reactions caused by Brucella and antigenically cross-reactive bacteria remains unresolved. The aim of the study was an application of a fluorescence polarization assay (FPA) for the examinations of sera from cattle for brucellosis. Four hundred fifty sera from cattle, including 300 sera from healthy animals, 27 sera from infected animals and 123 sera originated from confirmatory investigations, were used. The results obtained in FPA were compared to the results of RBT, SAT, CFT and ELISA. All sera from healthy animals were negative in FPA, whereas sera from infected animals were positive. Among sera from confirmatory investigations, 8 sera were positive in FPA. This likewise concerned sera positive both in CFT and ELISA. All sera positive only in RBT and SAT were negative in FPA. The results of the examinations show that FPA is a useful method for the diagnosis of brucellosis in cattle.

Diagnostyka i epidemiologia brucelozy bydła w Polsce

67%

Szulowski K.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

2012

tom 07

nr 2 Monografia