This study was conducted to evaluate the effects of combined deficiencies of Se and vitamin E on catalase activity in order to investigate the possible interactions between Se, α- tocopherol, and catalase in lambs with white muscle disease (WMD). Ten healthy and fifteen WMD affected lambs of the Akkaraman breed were used. Serum α-tocopherol and Se levels, and catalase activity were analysed. The catalase activity as well as α-tocopherol and Se levels in lambs with WMD before the treatment (day 0), were found to be lower than those of the control group (P<0.001). Therefore, the catalase activity should be taken into consideration in lambs with WMD.
Selenium level, and Ihe activity of GSH-Px, AST, CK, LDH and its isoenzymes were estimated in 30 lambs suspected of having the subclinical form of nutritional muscular dystrophy (NMD) and in 30 healthy lambs. A significant Se deficiency, a reduction in the activity of peroxidase glutathione, an increase in creatine kinase, aspartate aminotransferase and lactate dehydrogenase activities were found in the unhealthy lambs. Isoenzymatic profile of LDH showed 5 tractions. Tń the unhealthy lambs, there was a significant increase in LDH5 fraction-muscular isoenzyme, which is specific to the skeletal muscle. These indicators however, remained within a normal physiological range in the healthy lambs.
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