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The nucleotide sequence of the genomic RNA of a Polish isolate of a potato leafroll virus was determined. Some variations between the determined sequences were observed. A comparison of the frequency of sequence variants in particular regions of the genome is presented. The nucleotide sequence reported will appear in the EMBL Data Library under the accession number X74789.
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The caprine arthritis-encephalitis virus (CAEV) was isolated from monocyte-derived macrophages (M/M), but not from PBMC of seropositive goats by co-cultivation with goat synovial membrane cells. Out of eight M/M co-cultures, CAEV was evidenced by the syncytia formation and presence of proviral DNA in two and four cultures, respectively. Two virus isolates from co-cultures showing cytopathic effects were further confirmed as CAEV by western blotting, PCR, and sequence analysis. The nucleotide sequence of gag gene showed 92.0% and 90.3% homology to the prototype CAEV-Co strain. Supernatants harvested from these cultures induced syncytia when cultured with uninfected cells and the resultant titer was 1035 and 1025 TCID50 per ml. New CAEV isolates are suitable candidates for further analysis of their genetic and biological properties.
EN
Prior to this study, Trichomonas tenax (Müller, 1773) Dobell, 1939 has been described only from the human oral cavity, though other trichomonads found in dogs and cats (species in close contact with man) were morphologically very similar. Verification of T. tenax occurrence in synanthropic animals was therefore the aim of this study. PCR based molecular analysis of the isolates obtained from a dog and a cat confirmed the presence of T. tenax in these animals. This is the first report of natural infection of domestic animals with T. tenax.
EN
The nucleotide sequence of a chromosomal DNA fragment located upstream from the cysPTWAM operon of Escherichia coli was established. Sequence analysis indicates the presence of an open reading frame which has been designated ucpA (upstream cysE). The potential protein product exhibits strong sequence homology to the members of a large protein family, short-chain dehydrogenases/reductases. Involvement of Crp, FruR and IHF in the regula­tion of ucpA transcription in vivo was demonstrated.
EN
Six heat shock transcription factors (HSFs) have been isolated and characterized from soybean and two from Arabidopsis (Czarnecka-Verner et al. 1995; Barros, Czarnecka-Verner and Gurley, unpublished). Based on a phylogeny analysis of the DNA binding domains and organization of oligomerization domains, they have been assigned to classes A2 and B of the plant HSF family (Nover et al. 1996). In vivo studies of full length HSFs were conducted in transient expression systems using a GUS reporter driven by a heat shock element (HSE) located upstream from the minimal 35S CaMV promoter. Neither soybean nor Arabidopsis HSF class B members were able to function as transcriptional activators under control or heat stress conditions. Conversely, class A HSFs from tomato and Arabidopsis have the capacity to activate transcription. Co-expression studies of activator HSFs from tomato and Arabidopsis, and inert HSFs from soybean and Arabidopsis demonstrated that the inert HSFs were able to trans-attenuate the transcriptional activity of activator HSFs. We suggest that heat shock regulation in plants may differ from metazoan systems by partitioning negative and positive functional domains onto separate HSF proteins. In plants two classes of HSFs exist: class A members, i.e. HSF activators, and a novel class B (inert HSFs) which is largely specialized for repression, or attenuation, of the heat shock response.
EN
We conducted a parasitological study to identify nematodes infecting the gonads of bigeye barracuda Sphyraena forsteri Cuvier (Sphyraenidae) caught in the West Philippine Sea, off the Mariveles coast, Bataan Province, the Philippine archipelago. Males and very tiny nongravid female philometrid nematodes were collected from the ovaries of the fish. Based on light and scanning electron microscope observations, and supported by molecular analyses of the partial nucleotide sequences of the 18S small subunit ribosomal DNA, the philometrid nematode was determined to be a new species belonging to the genus Philometra Costa, 1845 in the family Philometridae (Nematoda: Dracunculoidea), and was named P. philippinensis sp. nov. This new species was clearly distinguishable morphologically from other congeners that parasitize the gonads of marine fishes. Males have unique sickle-shaped spicules and gubernaculum, and a vulva-like cloacal opening. Following Dentirumai philippinensis Quiazon et Moravec, 2013 and Philometra robusta Moravec, Möller et Heeger, 1992, this is the third philometrid species and the first gonad-infecting philometrid from the Philippine archipelago.
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