Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

2 Acta Biochimica Polonica

2 1996

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: nucleoside kinase

Sortuj według:

Ogranicz wyniki do:

The NTP phosphate donor in kinase reactions: Is ATP a monopolist?

100%

Shugar D.

nr 1

This brief overview describes some of the properties of various cellular phosphotransferase systems, with particular emphasis on nucleoside 5'-triphosphate (NTP)- dependent protein kinases and nucleoside kinases, for which it is widely and implicitly assumed that ATP is the intracellular phosphate donor. Numerous examples are presented, based on the in vitro properties of these enzymes, to show that ATP is not the only, or frequently not even the major, phosphate donor, and that this is probably reflected in vivo. It is pointed out that in vitro studies of donor and acceptor specificities of kinases must take account of the intracellular concentrations of nucleoside 5-triphosphates, a problem also relevant to the design of nucleoside analogues as chemotherapeutic agents. Attention is also drawn to NTP analogues as substrates/ inhibitors of protein kinases, and to several examples of low-molecular mass non- peptide substrates of these enzymes.

Stucture-activity relationships for phosphorylation of nucleoside analogs to monophosphates by nucleoside kinases

84%

Johansson N. G. , Eriksson S.

nr 1

The mammalian deoxyribonucleoside kinases thymidine kinase 1 and 2, deoxy- cytidine kinase and deoxyguanosine kinase phosphorylate deoxyribonucleosides and provide an alternative to de novo synthesis of DNA precursors. Their activities are essential for activation of several chemotherapeutically important nucleoside analogs. These four salvage kinase enzymes exhibit distinct substrate specificities for nucleoside analogs modified in the base and glycon moieties. In this review their, structure-activity relationships are discussed. Alternative routes for phosphorylation of nucleoside analogs are also reviewed, such as the phosphotransfer capacity of 5'-nucleotidase and protein kinases.