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EN
Hypothesis of phenotype matching and association are the most likely mechanisms in kin recognition. These hypotheses were tested by observing behavioral responses of cross-fostered root voles to urine cues from familiar and unfamiliar kin and non-kin. In experiment I, approach latency by males was significantly longer in response to non-sibling reared together (NSRT) than to non-sibling reared apart (NSRA) females, whereas, investigatory and sniffing time were significantly less in response to NSRT than to NSRA. However females showed no event bias to odors from NSRT and NSRA males. In experiment II, the behavioral responses of males and females to urine odors from siblings reared apart (SRA) and NSRA were not related to the degree of genetic relationship. It was concluded that: Association mechanism is used by male root voles in opposite-sex sibling recognition; Female voles possibly adopt multiple recognition mechanisms in different social tests.
EN
In this experiment, we tested the hypothesis that males of root voles (Microtus oeconomus Pallas) of different social ranks display different behavioural strategies. To document behavioural differences between social ranks, we investigated patterns in the behavioural responses to urine cues from familiar and novel individuals in a choice maze. Ten pairs of male voles were effectively used in this experiment. All behaviour was recorded with OBSERVER 5.0. When experiment was finished, video tapes were transformed into digital data. Then all data were analyzed by SPSS. The results showed that the approach latency of subordinates was shorter for familiar odours than novel ones, dominant individuals preferentially entered the strange odourant box, subordinates preferred familiar odours over novel ones, subordinates spent more time visiting familiar odours compared to the novel odours, dominants preferred novel odours to familiar ones, subordinates approached familiar odours more frequently than novel ones and self-groomed more often in the familiar odourant box than in the novel box, and dominant and subordinate individuals showed significantly different countermarking behaviours to familiar and novel odours. In conclusion, the dominants and subordinates displayed different behaviour patterns when faced to familiar and novel conspecific males' urine cues. The data support our hypothesis that differences in social rank induce differences in behavioural patterns.
EN
Urine is a major source of mammalian chemosignals. Among rodents, the sexual attractiveness and chemical constituents of urine vary with different reproductive stages. We confirmed the differing sexual attractiveness to males of the urine of lactating and non-lactating female root voles (Microtus oeconomus) and analyzed individual coding forms and lactation-specific putative pheromones, using gas chromatography-mass spectrometry (GC-MS). First, we documented the behavioral preference of male voles to urine odors of lactating and non-lactating females in a choice maze. The results showed that male voles engaged in more sniffing behavior and spent more time self-grooming in response to urinary odors of lactating females than to urinary odors of non-lactating females. We then used GC-MS to analyze the urine.s individual coding forms and potential chemosignals. We identified 34 volatile compounds, corresponding to 34 GC peaks, in the urine of female voles. The components identified in the urine samples included benzo- forms, alkanes, alkenes, acids, esters, pyrans, alcohols, and other volatile compounds. 12 basic volatiles were detected in all urine samples while others were specific to individuals. (E)5-octadecene and (N) hexadecanoic acid were specific to the lactating stage. A quantitative comparison of the relative abundances of the basic GC peaks showed no difference between the lactating and non-lactating stages, suggesting that individual identity is coded in digital form. We suggest that the urine of lactating females possesses stronger sexual attraction cues because of the presence of (E)5-octadecene and (N)hexadecanoic acid, which are putative lactating pheromones. However, the specific function of the pheromones should be assessed further by bioassay.
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