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EN
The aim of the study was to determine whether the character of a histopathological change observed in the epididymis of a 13-year-old European bison male was neoplastic, inflammatory or proliferous. Samples from both testes and epididymes were collected. The material was fixed in Bouin’s fluid; 6 mm paraffin sections were stained with hematoxylin and eosin (H+E), as well as by Brachet’s method. Full blood samples were collected from the femoral artery (post mortem). Immunocytochemical reactions were performed by the EnVisionTM FLEX+ technique. Specific monoclonal mouse and polyclonal rabbit antibodies were used against: vimentin (No IS630); human cytokeratin (No IS053); human CD 34 (No IS632); human desmin (No IS606); human smooth muscle myosin heavy chain (No IS066); myogenin (No IS067); human muscle actin (No M0635) in a 1:50 dilution; human MyoD1 (No M3512) in a 1:50 dilution; human myeloid/histiocyte (No M0747) in a 1:100 dilution; human receptor Cd 68 (No IS609); S-100 (No IS504). Micromorphometric measurements were carried out to assess the extent of connective tissue hyperplasia in the epididymis. The histopathological change in the epididymis of the 13-year-old bull was progressive and caused by chronic inflammation, which was confirmed by the micromorphometric analysis and the evidence of plasma cells and connective tissue hyperplasia. Immunohistochemical staining carried out to find vimentine receptors showed mesenchymatic cells forming disseminated foci in the epididymis. The appearance of the cells that made up the microgranulomas indicated their epithelioid nature. These transformed macrophages were devoid of the typical receptors Cd68 and Mac 387. However, the presence of spermatozoa in the foci suggested their phagocytic properties. Damage to the canaliculi of the epididymis and their phagocytosis could have been caused by an unknown proinflammatory factor. It is also likely that the destruction was induced by epithelioid cell proteins, which resulted in a dislocation of sperm to the epididymal stroma.
RU
Провели морфометрический сравнительный анализ яичек и придатков яичек молодых 1,5—3-летних хряков с правильным семенем и старых 4—8-летыих хряков, показывающих различного рода расстройства в составе семени. Отметили, что наиболее сильными изменениями в яичках старых неплодных хряков были: уменьшение их массу, дегенерация эпителия семенных канальцев с одновременным утолщением основной мембраны, образование сперматоцелей и отрофия семенных канальцев с разрастанием интерстициальных клеток. В придатках яичек наблюдали гиперплазию эпителия протока, образование интраэпителиальных кист, реже дегенерацию эпителия протока. Другие часто встречаемые у остальных видов старические изменения яичек были здесь слабо выражены либо не появлялись.
EN
There were performed comparative examinations concerning the testicles and epididymis of young boars (aged from 1.5 to 3 years) characterized by a normal semen and old boars, aged from 4 to 8 years showing different disturbances in the content of their semen. It was foud that the changes in the testicles of old boars characterized a decreased mass of the testicles of old sterile boars, degeneration of epithelial cells of the seminal tract and callosity of the basal membrane, spermatocoele and atrophia of the semen canal with interstitial hyperplasia. Hyperplasia in the epithelium of the epididymis tract, the presence of cysts and less often degeneration of the epithelium were observed. Other changes often seen in old testicles were mild or were not present at all.
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