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PL
Badania ścian naczyń krwionośnych metodą spektroskopii Ramana, pozwalają na badanie zależności między obciążeniem jakiemu poddawana jest tkanka, a jej strukturą. W celu zwiększenia efektywności pomiarów podjęta została próba zautomatyzowania układu służącego do rozciągania próbek poddawanych badaniu.
EN
Study of vessels walls by means of Raman Spectroscopy allows to analyze the relation between load placed upon the tissue and it’s structure. To maximize the effectiveness of measurement, the goal of automation of the tensile tool used in the procedure was undertaken.
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Content available remote Vascular texture modeling for image interpretation
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EN
In this paper, it is shown that pathological changes of vascular networks, inside an extensive organ, influence textures observed on medical images. A 3D, dynamic model of the organ and its vascular system was developed, and it enables to control the tissue perfusion. To render CT scan-like images, the acquisition process was also modeled. The proposed approach is applied to simulate the development of the hepatic vascular system and to induce hyper- and hypo-vascularized lesions inside the liver. The texture analysis of the obtained images is then performed. It shows that textural features can characterize regions changed by pathological processes as far as the acquisition conditions are precisely are presicely controlled.
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Content available remote Experimental analysis of steady and pulsating flows in collapsible walls
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EN
An experimental set-up to measure the changes of geometry of elastic walls and input and output pressures for constant and pulsating flow reflecting blood flow in human arteries was designed, built and tested. The paper presents the results of extensive experiments of static and pulsating flow parameters as functions of flow rates and pulsation frequencies for various properties of tested tubes. Aperiodic time series of pressure due to occurrence of turbulent flow have been observed.
PL
Przedstawiono wyniki badań eksperymentalnych przepływu statycznego i pulsacyjnego w elastycznych ściankach odwzorowującego przepływ w naczyniach krwionośnych. Zaprojektowano, zbudowano i przetestowano aparaturę umożliwiającą pomiar on-line zmian średnicy badanej rurki wykonanej z elastycznego materiału i ciśnień dynamicznych przed i za badaną próbką w zależności od natężenia prze-pływu i częstotliwości pulsacji. Zaobserwowano m.in. aperiodyczne zależności czasowe ciśnienia i średnicy rurki wynikające z burzliwego charakteru przepływu płynu.
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Content available remote The biomechanics of atherosclerosis development
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EN
In this paper, we investigate the bio-mechanics of atherosclerosis development in human physiology. Blood is modelled as an incompressible fluid of variable viscosity flowing in a slightly diverging channel (i.e. large artery) of small aspect ratio [1]. The hypothetical viewpoint in this work is the existence of relationship between the atherosclerosis development, blood viscosity, flow separation and turning points in the flow field. The problem is tackled by asymptotic approximation and the graphical results are discussed quantitatively.
PL
W pracy przedstawiono wpływ ładunku elektrycznego na adhezję krwinek płytkowych i poziom białka całkowitego. Wyznaczono potencjał zeta protez nieelektryzowanych i elektretów w roztworach o różnych pH. Zbadano wpływ czasu na potencjał zeta krwi ludzkiej. Określono zachowanie się potencjału zeta na granicy proteza naczyń krwionośnych-krew w funkcji czasu.
EN
Presented work describes influence of charge on blood platelets adhesion and complete protein level. Zeta potential of prostheses and electrets in solutions with different pH was determined. The influence of time on zeta potential of human blood was examined. Also behavior of zeta potential in function of time between blood vessel prosthesis – blood was described.
EN
The thermal interactions between the blood vessel and surrounding biological tissue are analyzed. The tissue temperature is described by the Pennes equation, while the equation determining the change of blood temperature along the blood vessel is formulated on the basis of adequate energy balance. These equations are coupled by a boundary condition given at the blood vessel wall. The problem is solved using the hybrid algorithm, this means the temperature field in biological tissue is determined by means of the boundary element method (BEM), while the blood temperature is determined by means of the finite difference method (FDM). In the final part the examples of computations are presented.
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Content available Najczęstsze powikłania w zabiegach ortognatycznych
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EN
Dentition and the remaining tissues forming the oral cavity, adequate prophylaxis, as well as providing opportunities for quick specialist dental treatment have a significant impact on the condition of practically the entire human body. It equally (and even particularly) concerns professional athletes and individuals involved in high level physical activity. Professional training carried out every day (and frequently twice daily) to exhaustion of nearly the entire psycho-physical potential of the human body affects not only the physical capacity of athletes, but also their results in sports. Involvement in the above mentioned sport disciplines predisposes to conditions which should be necessarily subjected to periodic dental consultation. These include 1. extreme and contact sports, such as: skateboarding, snowboarding, mountain biking, boxing. kick boxing and other combat sports; 2. car and motorcycle riding, aerobatics, motorized water sports, body building, etc., exposure to vibration and bruxism; 3. sport disciplines involving exposure to changes in pressure, such as mountain climbing and skydiving, damage to calcified dental tissues, pulpitis, etc. The literature review, as well as practical observation, indicate that most of the individuals involved in “less dangerous” sports, such as basketball, football, handball and water polo do not use teeth protectors , which results in tooth loss and jaw sprains of fractures. The authors presents the increasingly popular method of treatment, applied in patients with complex malformations of the facial skeleton and complex orthodontic deformations, requiring surgical orthodontic procedure performance by a surgical care team.
PL
Stan uzębienia i pozostałych tkanek tworzących jamę ustną, właściwa profilaktyka, a także zapewnienie możliwości szybkiego wdrożenia specjalistycznego leczenia stomatologicznego wpływają w istotny sposób na stan zdrowia praktycznie całego organizmu człowieka. Dotyczy to w równej mierze (a nawet w sposób szczególny) sportowców, zarówno wyczynowych, jak również realizujących aktywność fizyczną o wysokiej intensywności. Profesjonalny trening, wykonywany zazwyczaj codziennie (a często 2 razy dziennie), na granicy możliwości psychofizycznych organizmu, wpływa nie tylko na ogólny stan zdrowia, ale także na wydolność sportowca i osiągane wyniki. Stomatologia „sportowa”, chociaż nie stanowi odrębnej specjalizacji medycznej, stanowi bardzo ważny i integralny element medycyny sportowej. Uprawianie poniżej wymienionych dyscyplin sportu predysponuje do wystąpienia dolegliwości, które należy bezwzględnie poddawać okresowej konsultacji stomatologicznej: 1. sporty ekstremalne i kontaktowe: skateboarding, snowboarding, kolarstwo górskie, boks, kick boxing i pozostałe sporty walki – urazy, 2. rajdy samochodowe, motocyklowe, akrobacje lotnicze, sporty motorowodne, kulturystyka i in.– ekspozycja na wibracje i bruksizm, 3. dyscypliny sportu związane ze zmianami ciśnienia: nurkowanie, wspinaczka wysokogórska, skydiving-uszkodzenia tkanek twardych zębów, zapalenie miazgi itp. Przegląd literatury, jak również obserwacje praktyczne ukazują, że większość osób uprawiających „mniej groźne” sporty, takie jak: koszykówka, piłka nożna, ręczna i wodna, nie korzysta z ochraniaczy na zęby, czego konsekwencją w dyscyplinach uznawanych za względnie bezpieczne może być utrata zęba, zwichniecie żuchwy lub jej złamania. W artykule autorzy prezentują coraz powszechniejszą metodę leczenia pacjentów ze złożonymi wadami szkieletu czaszkowo-twarzowego, skomplikowanymi deformacjami ortodontycznymi, które wymagają zespołowego leczenia chirurgiczno-ortodontycznego pod nazwą ortognatyka.
EN
Retinal images play an important role in the early diagnosis of diseases such as diabetes. In the present study, an automatic image processing technique is proposed to segment retinal blood vessels in fundus images. The technique includes the design of a bank of 180 Gabor filters with varying scale and elongation parameters. Furthermore, an optimization method, namely, the imperialism competitive algorithm (ICA), is adopted for automatic parameter selection of the Gabor filter. In addition, a systematic method is proposed to determine the threshold value for reliable performance. Finally, the performance of the proposed approach is analyzed and compared with that of other approaches on the basis of the publicly available DRIVE database. The proposed method achieves an area under the receiver operating characteristic curve of 0.953 and an average accuracy of up to 0.9392. Thus, the results show that the proposed method is well comparable with alternative methods in the literature.
9
Content available remote A hybrid method for blood vessel segmentation in images
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EN
In the last years, image processing has been an important tool for health care. The analysis of retinal vessel images has become crucial to achieving a better diagnosis and treatment for several cardiovascular and ophthalmological deceases. Therefore, an automatic and accurate procedure for retinal vessel and optic disc segmentation is essential for illness detection. This task is extremely hard and time-consuming, often requiring the assistance of human experts with a high degree of professional skills. Several retinal vessel segmentation methods have been developed with satisfactory results. Nevertheless, most of such techniques present a poor performance mainly due to the complex structure of vessels in retinal images. In this paper, an accurate methodology for retinal vessel and optic disc segmentation is presented. The proposed scheme combines two different techniques: the Lateral Inhibition (LI) and the Differential Evolution (DE). The LI scheme produces a new image with enhanced contrast between the background and retinal vessels. Then, the DE algorithm is used to obtain the appropriate threshold values through the minimization of the cross-entropy function from the enhanced image. To evaluate the performance of the proposed approach, several experiments over images extracted from STARE, DRIVE, and DRISHTI-GS databases have been conducted. Simulation results demonstrate a high performance of the proposed scheme in comparison with similar methods reported in the literature.
EN
In the paper the heat transfer within the tissue in the presence of a pair of blood vessels is analyzed. Taking into account the of a blood vessel and inconsiderable change of blood temperature along the vessel one can consider the 2D problem. In order to solve the boundary element method is used. In the final part of the paper the example of computations is presented.
PL
W pracy rozważano przepływ ciepła w tkance biologicznej w obecności pary naczyń krwionośnych. Biorąc pod uwagę wymiary pojedynczego naczynia i niewielką zmianę temperatury krwi na jego długości, zadanie traktowano jako problem 2D. Do rozwiązania wykorzystano metodę elementów brzegowych. W końcowej części pracy przedstawiono przykład obliczeń numerycznych.
PL
Głównym celem tej pracy jest wyznaczenie właściwości mechanicznych ścian zdrowej aorty brzusznej oraz ścian tętniaka aorty brzusznej. W tym celu, przeprowadzono test jednoosiowego rozciągania próbek, które wycinano w dwóch kierunkach: wzdłużnym i obwodowym. Dla wszystkich przebadanych próbek wykreślano charakterystyki σ=f(ε) i wyznaczano dla nich umowny moduł Younga oraz wytrzymałość mechaniczną na rozciąganie. Zaobserwowano, że ściany tętniaka aorty brzusznej w porównaniu ze ścianami zdrowej aorty brzusznej są sztywniejsze.
EN
The maim aim of this research work is appointed mechanical properties of walls of normal abdominal aorta and walls of abdominal aortic aneurysms. In this end of view, the uniaxial tensile tests were performed for each specimen, which were cut out research material in two directions: longitudinal and circumferential. On the basis these tests the stress-strain characteristics were drawn for all specimens and the assumed Young's modules and the tensile strength were assigned. The results indicate that the walls of abdominal aortic aneurysms in comparison with walls of normal abdominal aortas are stiffer.
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EN
Automated segmentation of optic disc in fundus images plays a vital role in computer aided diagnosis (CAD) of eye pathologies. In this paper, a novel method is proposed which detects and excludes the blood vessel for accurate optic disc segmentation. This is achieved in two steps. First, an effective blood vessel detection and exclusion algorithm is developed using directional filter. In the second step, a decision tree classifier is used to obtain an adaptive threshold in order to detect the contour of optic disc. The proposed method aids in computationally robust segmentation of optic disc even in fundus images having illuminations, reflections and exudates. The proposed method is tested on two different datasets which includes 300 fundus images collected from Kasturba Medical College (KMC) Manipal and also the publically available RIM-ONE database. The average values of Jaccard index, dice coefficient, sensitivity, specificity and accuracy obtained for KMC images is 91.28 %, 94.17 %, 92.71 %, 99.89 % and 99.61 % respectively. For RIM-ONE database the obtained average values of Jaccard index, dice coefficient, sensitivity, specificity and accuracy are 85.30 %, 90.69 %, 93.90 %, 99.39 % and 99.15 % respectively. The obtained segmentation results proves the efficiency of the algorithm to be incorporated in CAD of eye diseases.
13
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EN
The change in vascular geometry is an indicator of various health issues linked with vision and cardiovascular risk factors. Early detection and diagnosis of these changes can help patients to select an appropriate treatment option when the disease is in its primary phase. Automatic segmentation and quantification of these vessels would decrease the cost and eliminate inconsistency related to manual grading. However, automatic detection of the vessels is challenging in the presence of retinal pathologies and non-uniform illumination, two common occurrences in clinical settings. This paper presents a novel framework to address the issue of retinal blood vessel detection and width measurement under these challenging circumstances and also on two different imaging modalities: color fundus imaging and Scanning Laser Ophthalmoscopy (SLO). In this framework, initially, vessel enhancement is done using linear recursive filtering. Then, a unique combination of morphological operations, background estimation, and iterative thresholding are applied to segment the blood vessels. Further, vessel diameter is estimated in two steps: firstly, vessel centerlines are extracted using the graph-based algorithm. Then, vessel edges are localized from the image profiles, by utilizing spline fitting to obtain vascular orientations and then finding the zero-crossings. Extensive experiments have been carried out on several publicly accessible datasets for vessel segmentation and diameter measurement, i.e., DRIVE, STARE, IOSTAR, RC-SLO and REVIEW dataset. Results demonstrate the competitive and comparable performance than earlier methods. The encouraging quantitative and visual performance of the proposed framework makes it an important component of a decision support system for retinal images.
14
Content available remote Endothelial cell responses to fluid shear stress
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EN
Implications of vascular endothelial cell (EC) responses to flow-oriented wall shear stress are outlined from the bioengineering viewpoint. These responses regulate the adaptive remodeling of the vascular system including capilIary networks, in a negative feedback mechanism maintaining the shear stress level constant. The preserved set point of the stress is selected so as to optimize the mass transport function of the system. To initiate such shear-dependent responses, EC has a mechano-sensor conjugated with second messengers, one of which is cytoplasmic Ca(2+) transient. The evidence that wall shear stress is the real factor inducing the transient, is visualized from an in vitro flow loading experiment to cultured ECs using mediums differing in viscosity.
PL
W artykule przedstawiono implikacje występowania reakcji komórki śródbłonka na naprężenia ścinające w ścianie naczynia, związane z przepływem płynu. Reakcje te kontrolują adaptację systemu naczyń krwionośnych, łącznie z siecią kapilar, utrzymując naprężenia ścinające na stałym poziomie za pomocą mechanizmu ujemnego sprzężenia zwrotnego. Zadany poziom naprężeń jest tak dobrany, aby optymalizować funkcję transportu masowego układu naczyniowego. W celu inicjacji reakcji, zależnej od poziomu naprężeń ścinających, komórki nabłonka wykorzystują mechano-sensor połączony z innymi "przekaźnikami", z których jednym jest transport wapnia. Dowód, że naprężenia ścinające w ściance naczyń są czynnikiem uruchamiającym ten "transport" jest widoczny podczas badań in vitro mechanizmu przepływu medium o różnej lepkości w hodowli komórek śródbłonka.
15
Content available remote Modelling of pulsatory flows in blood vessels
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The paper deals with the method of modelling of blood pulsatory flow in the vessels of circulatory system. The method is based upon the solution of wave equation applied in the theory of four-pole. In a graphic presentation of a vessel of distributed parameters, a model of bond graphs with new elements of DB type ise used. Basic physiological simulation experiment using bond graphs was performed. The aim of the experiment was to model the changes of pressure and volume in a left heart-aorta segment.
EN
Most of the retinal diseases namely retinopathy, occlusion etc., can be identified through changes exhibited in retinal vasculature of fundus images. Thus, segmentation of retinal blood vessels aids in detecting the alterations and hence the disease. Manual segmentation of vessels requires expertise. It is a very tedious and time consuming task as vessels are only a few pixels wide and extend almost throughout entire span of the fundus image. Employing computational approaches for this purpose would help in efficient retinal analysis. The methodology proposed in this work involves sequential application of image pre-processing, supervised and unsupervised learning and image post-processing techniques. Image cropping, color transformation and color channel extraction, contrast enhancement, Gabor filtering and halfwave rectification are sequentially applied during pre-processing stage. A feature vector is formed from the pre-processed images. Principal component analysis is performed on the feature vector. K-means clustering is executed on this outcome to group pixels as either vessel or non-vessel cluster. Out of the two groups, the identified non-vessel group undergoes an ensemble classification process employing root guided decision tree with bagging, while vessel group is left unprocessed as further processing might increase misclassifications of vessels as non-vessels. The resultant segmented image is formed through combining the results of clustering and ensemble classification process. The vessel segmented output from previous phase is post-processed through morphological techniques. The proposed technique is validated on images from publicly available DRIVE database. The proposed methodology achieves an accuracy of 95.36%, which is comparable with the existing blood vessel segmentation techniques.
PL
Wstęp. Ciała tłuszczowe stawu kolanowego są już relatywnie dobrze poznanymi strukturami z jednym wyjątkiem – ciałem tłuszczowym okołokrzyżowym (PCFP). Cel. Do celów badania należały: (1) dysekcja, opis i pomiar cech anatomicznych PCFP oraz (2) ocena obecności, morfologii i relacji tętnicy środkowej kolana (MGA) względem PCFP. Materiał i metody. Dysekcji zostały poddane cztery kończyny dolne z męskich zwłok. PCFP zostało wycięte, a następnie zmierzono jego długość horyzontalną i wertykalną oraz objętość. Oceniono obecność, morfologię i relację MGA względem PCFP oraz zmierzono jej średnicę wewnętrzną. Wyniki. PCFP było zlokalizowane w dole międzykłykciowym. Granice PCFP stanowiły od strony górnej, dolnej, przyśrodkowej i bocznej oraz tylnej kolejno dach dołu międzykłykciowego, plateau piszczeli, kłykcie udowe i tylna torba stawowa. Przednia część PCFP otaczała więzadło krzyżowe tylne (PCL), do którego przyłączone było włóknistą wypustką. PCFP miało konsystencję homogennej tkanki tłuszczowej z małymi płacikami. Średni wymiar horyzontalny, wertykalny i objętość wyniosły kolejno 56,85±3,4 mm (zakres wartości 52,1-60,2 mm), 42,8±5,8 mm (zakres wartości 34,2-46,1 mm) i 43,75±4,79 ml (zakres wartości 40-50 ml). MGA przeszywała wszystkie 4 ciała tłuszczowe w górno-tylnym brzegu a jej średnica wewnętrzna wyniosła 1,18±0,33 mm (zakres wartości 0,8-1,55 mm). Wnioski. PCFP jest stosunkowo dużą strukturą z MGA obecną w każdym z 4 badanych przypadków. Należy zachować dodatkową ostrożność podczas operacji tylnego przedziału kolana, ponieważ PCFP może wykazywać niedoceniany potencjał do obrzęku i krwawienia.
EN
Introduction. Fat pads of the human knee joint are relatively well-known structures except for the pericruciate fat pad (PCFP). Aim. The study aimed to describe (1) the anatomy of the pericruciate fat pad (PCFP) and (2) its relation to the middle genicular artery (MGA). Materials and methods. Four male cadaveric lower limbs were dissected. PCFP was excised, and its horizontal and vertical lengths and volume were measured. The presence of MGA was assessed, and its internal diameter was registered. Results. PCFP was located in the intercondylar fossa. Its superior, inferior, medial&lateral and posterior borders were the intercondylar roof, tibial plateau, femoral condyles, and posterior knee capsule, respectively. The anterior part of the PCFP enveloped the posterior cruciate ligament (PCL). PCFP had a homogenous appearance of adipose tissue with small lobules. It was connected to the PCL with a fibrous process. The mean horizontal&vertical length and volume were 56.85±3.4 mm (range 52.1-60.2 mm), 42.8±5.8 mm (range 34.2-46.1 mm) and 43.75±4.79 ml (range 40-50 ml), respectively. The MGA penetrated the PCFP in all four examined limbs; its mean diameter was 1.18±0.33 mm (range 0.8-1.55 mm). Conclusions. PCFP is a relatively large structure, with MGA present within all examined cases. Care must be taken when performing surgeries involving the posterior intra-articular part of the knee because the PCFP may present an underestimated potential for oedema and bleeding.
18
Content available remote CNN-based superresolution reconstruction of 3D MR images using thick-slice scans
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EN
Due to inherent physical and hardware limitations, 3D MR images are often acquired in the form of orthogonal thick slices, resulting in highly anisotropic voxels. This causes the partial volume effect, which introduces blurring of image details, appearance of staircase artifacts and significantly decreases the diagnostic value of images. To restore high resolution isotropic volumes, we propose to use a convolutional neural network (CNN) driven by patches taken from three orthogonal thick-slice images. To assess the validity and efficiency of this postprocessing approach, we used 1x1x1 mm3-voxel brain images of different modalities, available via the well known BrainWeb database. They served as a high resolution reference and were numerically preprocessed to create input images of different slice thickness and anatomical orientation, for CNN training, validation and testing. The visual quality of reconstructed images was indeed superior, compared to images obtained by fusion of interpolated thick-slice images, or to images reconstructed with the CNN using a single input MR scan. The significant increase of objectively computed figures of merit, e.g. the Structural Similarity Image Metric, was also noticed. Keeping in mind that any single value of such quality metrics represents a number of psychophysical effects, we applied the CNN trained on brain images for superresolution reconstruction of synthetic and acquired blood vessel tree images. We then used the restored superresolution volumes for estimation of vessel radii. It was demonstrated that vessel radius values derived from superresolution images of simulated vessel trees are significantly more accurate than those obtained from a standard fusion of interpolated thick-slice orthogonal scans. Superiority of the CNN-based superresolution images was also observed for scanner-acquired MR scans according to the evaluated parameters. These three experiments show the efficiency of CNN-based image reconstruction for qualitative and quantitative improvement of its diagnostic quality, as well as illustrates the practical usefulness of transfer learning - networks trained on example images of one kind can be used to restore superresolution images of physically different objects.
EN
In image processing, models are used to improve robustness of algorithms by introducing a priori knowledge. Deformable models, frequently used in the field of medical images, are described by means of energy functionals with data attachment terms and regularising terms. The regularising terms express constraints relating to the expected shapes. The expected shape of a blood vessel segment in 3D images obtained by Magnetic Resonance Imaging or by helicoidal Computed Tomography is often implicitly described by a generalised cylinder model, i.e. an association of an axis (vessel centreline) and a surface (vessel boundary). In this context, the data attachment terms involve, for candidate points, a measure of the likelihood of being located on the centreline or on the boundary. Such a measure can use models reflecting low-level local photometrical properties of the brightness patterns. This presentation will give an overview of the recently used models and will be illustrated by the authors' contribution.
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Content available remote Vascular network description and visualization
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EN
This paper describes a technigue of description and visualization of vasculature. Vascular description includes a topology structure, cross section area and volume measurements. Visualization interacting system allows studying extracted data.
PL
W artykule opisano technikę reprezentacji i wizualizacji sieci naczyń krwionośnych. System opisu takiej sieci zawiera strukturę topologiczną, przekroje i pomiary objętości. Współpracujący system wizualizacji umożliwia analizę wydobytych danych.
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